Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal
Glucoside is the main antinutritional factor (ANF) of rapeseed meal. In the present study, a bacterium with high efficiency in degrading rapeseed glucoside was screened from pond water. The initial concentration of 1.0 × 107 CFU/mL bacterial solution was added to rapeseed meal and fermented for 48 h...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/anu/3661772 |
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| author | Hong-yu Lu An-Ning Shi Jun Wang Li-Juan Liu Jiang-Yi Tang Ya-Jun Wang Xue-Zhi Zhu De-Feng Zhang Li-Yan Liu Qing Wang |
| author_facet | Hong-yu Lu An-Ning Shi Jun Wang Li-Juan Liu Jiang-Yi Tang Ya-Jun Wang Xue-Zhi Zhu De-Feng Zhang Li-Yan Liu Qing Wang |
| author_sort | Hong-yu Lu |
| collection | DOAJ |
| description | Glucoside is the main antinutritional factor (ANF) of rapeseed meal. In the present study, a bacterium with high efficiency in degrading rapeseed glucoside was screened from pond water. The initial concentration of 1.0 × 107 CFU/mL bacterial solution was added to rapeseed meal and fermented for 48 h with a degradation rate of glucoside and isothiocyanate of 67% and 54%, respectively. BSY82 was identified by PacBio third-generation sequencing, and its biology was analyzed. Results showed that BSY82 belongs to Bacillus subtilis. The total length of genome was 4,242,094 bp, among which 89% was coding genes (3,777,298 bp). No virulence factors were predicted, based on a comparative analysis of the strain’s coding genes against known virulence factor databases. Functional analysis showed that BSY82 had the ability to produce myrosinase. The activity of myrosinase in the crude enzyme solution of the strain was 1.67 and 2.12 μmol/min by spectrophotometry and high-performance liquid chromatography (HPLC). The results indicated that BSY82 strain could be used as the optimal material in rapeseed meal fermentation detoxification process. |
| format | Article |
| id | doaj-art-2f7efa7c2ebc45baadac0ec2048a130f |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-2f7efa7c2ebc45baadac0ec2048a130f2025-08-20T03:59:25ZengWileyAquaculture Nutrition1365-20952025-01-01202510.1155/anu/3661772Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed MealHong-yu Lu0An-Ning Shi1Jun Wang2Li-Juan Liu3Jiang-Yi Tang4Ya-Jun Wang5Xue-Zhi Zhu6De-Feng Zhang7Li-Yan Liu8Qing Wang9Pearl River Fisheries Research InstitutePearl River Fisheries Research InstituteKey Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze RiverPearl River Fisheries Research InstitutePearl River Fisheries Research InstitutePearl River Fisheries Research InstituteGuangdong Yuehai Feeds Group Co., Ltd.Pearl River Fisheries Research InstituteGuangdong Yuehai Feeds Group Co., Ltd.Pearl River Fisheries Research InstituteGlucoside is the main antinutritional factor (ANF) of rapeseed meal. In the present study, a bacterium with high efficiency in degrading rapeseed glucoside was screened from pond water. The initial concentration of 1.0 × 107 CFU/mL bacterial solution was added to rapeseed meal and fermented for 48 h with a degradation rate of glucoside and isothiocyanate of 67% and 54%, respectively. BSY82 was identified by PacBio third-generation sequencing, and its biology was analyzed. Results showed that BSY82 belongs to Bacillus subtilis. The total length of genome was 4,242,094 bp, among which 89% was coding genes (3,777,298 bp). No virulence factors were predicted, based on a comparative analysis of the strain’s coding genes against known virulence factor databases. Functional analysis showed that BSY82 had the ability to produce myrosinase. The activity of myrosinase in the crude enzyme solution of the strain was 1.67 and 2.12 μmol/min by spectrophotometry and high-performance liquid chromatography (HPLC). The results indicated that BSY82 strain could be used as the optimal material in rapeseed meal fermentation detoxification process.http://dx.doi.org/10.1155/anu/3661772 |
| spellingShingle | Hong-yu Lu An-Ning Shi Jun Wang Li-Juan Liu Jiang-Yi Tang Ya-Jun Wang Xue-Zhi Zhu De-Feng Zhang Li-Yan Liu Qing Wang Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal Aquaculture Nutrition |
| title | Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal |
| title_full | Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal |
| title_fullStr | Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal |
| title_full_unstemmed | Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal |
| title_short | Biological Characterization and Glucosinolate Degradation Mechanisms of Bacillus subtilis BSY82 in Rapeseed Meal |
| title_sort | biological characterization and glucosinolate degradation mechanisms of bacillus subtilis bsy82 in rapeseed meal |
| url | http://dx.doi.org/10.1155/anu/3661772 |
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