Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.
Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machin...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-04-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1003575&type=printable |
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| author | Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler |
| author_facet | Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler |
| author_sort | Adam M Feist |
| collection | DOAJ |
| description | Geobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species. |
| format | Article |
| id | doaj-art-2f7da3511c5a43a1a60fcf00f39f5dc2 |
| institution | DOAJ |
| issn | 1553-734X 1553-7358 |
| language | English |
| publishDate | 2014-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj-art-2f7da3511c5a43a1a60fcf00f39f5dc22025-08-20T03:01:22ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582014-04-01104e100357510.1371/journal.pcbi.1003575Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens.Adam M FeistHarish NagarajanAmelia-Elena RotaruPier-Luc TremblayTian ZhangKelly P NevinDerek R LovleyKarsten ZenglerGeobacter species are of great interest for environmental and biotechnology applications as they can carry out direct electron transfer to insoluble metals or other microorganisms and have the ability to assimilate inorganic carbon. Here, we report on the capability and key enabling metabolic machinery of Geobacter metallireducens GS-15 to carry out CO2 fixation and direct electron transfer to iron. An updated metabolic reconstruction was generated, growth screens on targeted conditions of interest were performed, and constraint-based analysis was utilized to characterize and evaluate critical pathways and reactions in G. metallireducens. The novel capability of G. metallireducens to grow autotrophically with formate and Fe(III) was predicted and subsequently validated in vivo. Additionally, the energetic cost of transferring electrons to an external electron acceptor was determined through analysis of growth experiments carried out using three different electron acceptors (Fe(III), nitrate, and fumarate) by systematically isolating and examining different parts of the electron transport chain. The updated reconstruction will serve as a knowledgebase for understanding and engineering Geobacter and similar species.https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1003575&type=printable |
| spellingShingle | Adam M Feist Harish Nagarajan Amelia-Elena Rotaru Pier-Luc Tremblay Tian Zhang Kelly P Nevin Derek R Lovley Karsten Zengler Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. PLoS Computational Biology |
| title | Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. |
| title_full | Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. |
| title_fullStr | Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. |
| title_full_unstemmed | Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. |
| title_short | Constraint-based modeling of carbon fixation and the energetics of electron transfer in Geobacter metallireducens. |
| title_sort | constraint based modeling of carbon fixation and the energetics of electron transfer in geobacter metallireducens |
| url | https://journals.plos.org/ploscompbiol/article/file?id=10.1371/journal.pcbi.1003575&type=printable |
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