Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence
Aristolochia baetica (A. baetica) is a wild species of Aristolochiaceae family; its roots are used by Moroccan people against cancer for many years ago. The objective of the study was to investigate the phytochemical screening, acute and subacute toxicity of A. baetica roots growing in the north of...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2019/8203832 |
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| author | Mohammed Bourhia Amal Ait Haj Said Ayoub Chaanoun Fatiha El Gueddari Abderrahim Naamane Laila Benbacer Naima Khlil |
| author_facet | Mohammed Bourhia Amal Ait Haj Said Ayoub Chaanoun Fatiha El Gueddari Abderrahim Naamane Laila Benbacer Naima Khlil |
| author_sort | Mohammed Bourhia |
| collection | DOAJ |
| description | Aristolochia baetica (A. baetica) is a wild species of Aristolochiaceae family; its roots are used by Moroccan people against cancer for many years ago. The objective of the study was to investigate the phytochemical screening, acute and subacute toxicity of A. baetica roots growing in the north of Morocco. Qualitative and quantitative analyses of A. baetica roots were performed using standard methods; the acute toxicity of the root extract of the studied plant was assessed in mice by gavage of single doses of 1, 2, and 4 g/kg body weight for 14 days; by the time the subacute toxicity was done using repeated doses 1, 1.5, and 2 g/kg/day for 28 days. Histological changes and biochemical parameters as markers of kidney and liver function were evaluated. The results of phytochemical screening showed the presence of polyphenols, tannins, alkaloids, flavonoids, saponins, and the absence of anthraquinones, sterols, and terpenes. The results of acute toxicity showed the absence of mortality and signs of toxicity in groups treated with 1 and 2 g/kg; however, the clinical signs of toxicity were important and the rate of mortality was estimated at 16 % in the group treated with 4 g/kg. The results of subacute toxicity showed several changes of serum parameters registered in groups treated with 1.5 and 2 g/kg/day, respectively. The results showed also the absence of histological injuries in groups treated with 1 and 1.5 g/kg/day; meanwhile, the histological alterations were remarkable in treated group with the highest dose administered of 2 g/kg/day. The outcome of this work showed that the roots’ extract of the studied plant was toxic in mice with repeated doses, but no toxic effect was observed with a single dose under 4g/kg. |
| format | Article |
| id | doaj-art-2f7aeacd9d7048589e6a2a33d855f92a |
| institution | OA Journals |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-2f7aeacd9d7048589e6a2a33d855f92a2025-08-20T02:08:11ZengWileyJournal of Toxicology1687-81911687-82052019-01-01201910.1155/2019/82038328203832Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical EvidenceMohammed Bourhia0Amal Ait Haj Said1Ayoub Chaanoun2Fatiha El Gueddari3Abderrahim Naamane4Laila Benbacer5Naima Khlil6Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, MoroccoLaboratory of Pharmacognosy, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, MoroccoLaboratory of Health and Agri-Food, Faculty of Science and Technology, University Hassan I, Route de Casablanca Km. 3,5, BP 539, Settat, MoroccoLaboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, MoroccoLaboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, MoroccoLife Science Division, National Centre for Energy, Sciences, and Nuclear Techniques, BP 1382, 10001 Rabat, MoroccoLaboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University, 19 rue Tarik Ibn Ziad, Casablanca, MoroccoAristolochia baetica (A. baetica) is a wild species of Aristolochiaceae family; its roots are used by Moroccan people against cancer for many years ago. The objective of the study was to investigate the phytochemical screening, acute and subacute toxicity of A. baetica roots growing in the north of Morocco. Qualitative and quantitative analyses of A. baetica roots were performed using standard methods; the acute toxicity of the root extract of the studied plant was assessed in mice by gavage of single doses of 1, 2, and 4 g/kg body weight for 14 days; by the time the subacute toxicity was done using repeated doses 1, 1.5, and 2 g/kg/day for 28 days. Histological changes and biochemical parameters as markers of kidney and liver function were evaluated. The results of phytochemical screening showed the presence of polyphenols, tannins, alkaloids, flavonoids, saponins, and the absence of anthraquinones, sterols, and terpenes. The results of acute toxicity showed the absence of mortality and signs of toxicity in groups treated with 1 and 2 g/kg; however, the clinical signs of toxicity were important and the rate of mortality was estimated at 16 % in the group treated with 4 g/kg. The results of subacute toxicity showed several changes of serum parameters registered in groups treated with 1.5 and 2 g/kg/day, respectively. The results showed also the absence of histological injuries in groups treated with 1 and 1.5 g/kg/day; meanwhile, the histological alterations were remarkable in treated group with the highest dose administered of 2 g/kg/day. The outcome of this work showed that the roots’ extract of the studied plant was toxic in mice with repeated doses, but no toxic effect was observed with a single dose under 4g/kg.http://dx.doi.org/10.1155/2019/8203832 |
| spellingShingle | Mohammed Bourhia Amal Ait Haj Said Ayoub Chaanoun Fatiha El Gueddari Abderrahim Naamane Laila Benbacer Naima Khlil Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence Journal of Toxicology |
| title | Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence |
| title_full | Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence |
| title_fullStr | Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence |
| title_full_unstemmed | Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence |
| title_short | Phytochemical Screening and Toxicological Study of Aristolochia baetica Linn Roots: Histopathological and Biochemical Evidence |
| title_sort | phytochemical screening and toxicological study of aristolochia baetica linn roots histopathological and biochemical evidence |
| url | http://dx.doi.org/10.1155/2019/8203832 |
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