Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation
Background Recent studies suggest that the loss of lysosomal function is associated with acute kidney injury (AKI), potentially leading to impaired autophagy. Apelin has been known to regulate autophagy processes in cardiovascular and pulmonary diseases. We sought to explore its potential contributi...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Renal Failure |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2480243 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850095265130741760 |
|---|---|
| author | Hao Pan Shuangshuang Li Yong Ning Zhizhi Hu |
| author_facet | Hao Pan Shuangshuang Li Yong Ning Zhizhi Hu |
| author_sort | Hao Pan |
| collection | DOAJ |
| description | Background Recent studies suggest that the loss of lysosomal function is associated with acute kidney injury (AKI), potentially leading to impaired autophagy. Apelin has been known to regulate autophagy processes in cardiovascular and pulmonary diseases. We sought to explore its potential contribution in lysosomal function and autophagy modulation during AKI.Methods Apelin-13 (30 μg/kg) or a vehicle control was administered to mice intraperitoneally 24 h prior to and at 0 h, 24 h, and 48 h following renal ischemia-reperfusion (I/R) injury or a sham procedure. Kidney and serum samples were collected for analysis 24 or 72 h postoperatively.Results Our findings indicate that apelin-13 significantly mitigated renal damage and inhibited apoptosis post-AKI. Flow cytometry analysis revealed that apelin-13 treatment modulates the macrophages polarization within the kidney from M1 to M2 phenotype. Additionally, apelin-13 was found to reduce the expression of the (pro)renin receptor, restore lysosomal membrane permeability, augment lysosomal biogenesis, and enhance autophagic flux in the kidney following AKI.Conclusions Our study elucidates novel mechanisms underlying the protective effects of apelin in AKI through modulating lysosomal function and autophagy. |
| format | Article |
| id | doaj-art-2f796b7628ed471c91e6db7daec99655 |
| institution | DOAJ |
| issn | 0886-022X 1525-6049 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj-art-2f796b7628ed471c91e6db7daec996552025-08-20T02:41:30ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492025-12-0147110.1080/0886022X.2025.2480243Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulationHao Pan0Shuangshuang Li1Yong Ning2Zhizhi Hu3Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P. R. ChinaBackground Recent studies suggest that the loss of lysosomal function is associated with acute kidney injury (AKI), potentially leading to impaired autophagy. Apelin has been known to regulate autophagy processes in cardiovascular and pulmonary diseases. We sought to explore its potential contribution in lysosomal function and autophagy modulation during AKI.Methods Apelin-13 (30 μg/kg) or a vehicle control was administered to mice intraperitoneally 24 h prior to and at 0 h, 24 h, and 48 h following renal ischemia-reperfusion (I/R) injury or a sham procedure. Kidney and serum samples were collected for analysis 24 or 72 h postoperatively.Results Our findings indicate that apelin-13 significantly mitigated renal damage and inhibited apoptosis post-AKI. Flow cytometry analysis revealed that apelin-13 treatment modulates the macrophages polarization within the kidney from M1 to M2 phenotype. Additionally, apelin-13 was found to reduce the expression of the (pro)renin receptor, restore lysosomal membrane permeability, augment lysosomal biogenesis, and enhance autophagic flux in the kidney following AKI.Conclusions Our study elucidates novel mechanisms underlying the protective effects of apelin in AKI through modulating lysosomal function and autophagy.https://www.tandfonline.com/doi/10.1080/0886022X.2025.2480243Apelinacute kidney injurylysosomeautophagy |
| spellingShingle | Hao Pan Shuangshuang Li Yong Ning Zhizhi Hu Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation Renal Failure Apelin acute kidney injury lysosome autophagy |
| title | Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| title_full | Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| title_fullStr | Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| title_full_unstemmed | Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| title_short | Apelin-13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| title_sort | apelin 13 exerts protective effects against acute kidney injury by lysosomal function regulation |
| topic | Apelin acute kidney injury lysosome autophagy |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2025.2480243 |
| work_keys_str_mv | AT haopan apelin13exertsprotectiveeffectsagainstacutekidneyinjurybylysosomalfunctionregulation AT shuangshuangli apelin13exertsprotectiveeffectsagainstacutekidneyinjurybylysosomalfunctionregulation AT yongning apelin13exertsprotectiveeffectsagainstacutekidneyinjurybylysosomalfunctionregulation AT zhizhihu apelin13exertsprotectiveeffectsagainstacutekidneyinjurybylysosomalfunctionregulation |