Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures
<b>Background/Objectives</b>: Bicyclic monoterpenes are one of the most common groups of secondary plant metabolites found in Nature. Their wide spectrum of biological activity can be used in the prevention and in the treatment of various diseases, including so-called ‘diseases of civili...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/15/3/199 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850091544846008320 |
|---|---|
| author | Karolina Wojtunik-Kulesza Marcela Dubiel Katarzyna Klimek |
| author_facet | Karolina Wojtunik-Kulesza Marcela Dubiel Katarzyna Klimek |
| author_sort | Karolina Wojtunik-Kulesza |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Bicyclic monoterpenes are one of the most common groups of secondary plant metabolites found in Nature. Their wide spectrum of biological activity can be used in the prevention and in the treatment of various diseases, including so-called ‘diseases of civilization’. Their potential for synergistic interactions may influence the biological activities of more complex mixtures. <b>Methods</b>: This study investigated the ability of selected bicyclic monoterpenes and their binary mixtures to reduce Fe(III) and Cu(II) and chelate Fe(II) and assessed their cytotoxic activity against BJ and HepG2 cell lines. <b>Results</b>: The obtained results did not reveal synergistic interactions towards the biological activities, but binary mixtures proved to be safe in relation to the tested cell lines. Among the tested single monoterpenes, the most effective were 3-carene and <i>β</i>-pinene, with the latter exhibiting the greatest ability to decrease cell viability (CC<sub>50</sub> for BJ and HepG2 cells was about 1.08 and 1.85 mM, respectively). <b>Conclusions</b>: The results revealed that both single compounds and binary mixtures demonstrate the ability to reduce selected metal ions and chelate Fe(II) ions. Synergistic interactions were not observed, but an increase in the activity of selected binary mixtures was recorded. Based on cell culture experiments, the monoterpenes and their binary mixtures can be considered safe at a concentration lower than 1 mM and close to 0.313 mM, respectively. |
| format | Article |
| id | doaj-art-2f74a607ba6b4cd289dcb472fe308162 |
| institution | DOAJ |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-2f74a607ba6b4cd289dcb472fe3081622025-08-20T02:42:22ZengMDPI AGMetabolites2218-19892025-03-0115319910.3390/metabo15030199Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary MixturesKarolina Wojtunik-Kulesza0Marcela Dubiel1Katarzyna Klimek2Department of Inorganic Chemistry, Medical University of Lublin, Chodzki Street 4a, 20-093 Lublin, PolandDepartment of Inorganic Chemistry, Medical University of Lublin, Chodzki Street 4a, 20-093 Lublin, PolandChair and Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodzki Street 1, 20-093 Lublin, Poland<b>Background/Objectives</b>: Bicyclic monoterpenes are one of the most common groups of secondary plant metabolites found in Nature. Their wide spectrum of biological activity can be used in the prevention and in the treatment of various diseases, including so-called ‘diseases of civilization’. Their potential for synergistic interactions may influence the biological activities of more complex mixtures. <b>Methods</b>: This study investigated the ability of selected bicyclic monoterpenes and their binary mixtures to reduce Fe(III) and Cu(II) and chelate Fe(II) and assessed their cytotoxic activity against BJ and HepG2 cell lines. <b>Results</b>: The obtained results did not reveal synergistic interactions towards the biological activities, but binary mixtures proved to be safe in relation to the tested cell lines. Among the tested single monoterpenes, the most effective were 3-carene and <i>β</i>-pinene, with the latter exhibiting the greatest ability to decrease cell viability (CC<sub>50</sub> for BJ and HepG2 cells was about 1.08 and 1.85 mM, respectively). <b>Conclusions</b>: The results revealed that both single compounds and binary mixtures demonstrate the ability to reduce selected metal ions and chelate Fe(II) ions. Synergistic interactions were not observed, but an increase in the activity of selected binary mixtures was recorded. Based on cell culture experiments, the monoterpenes and their binary mixtures can be considered safe at a concentration lower than 1 mM and close to 0.313 mM, respectively.https://www.mdpi.com/2218-1989/15/3/199bicyclic monoterpenescytotoxicityhepatocytesfibroblastsmetal ion reductionchelation |
| spellingShingle | Karolina Wojtunik-Kulesza Marcela Dubiel Katarzyna Klimek Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures Metabolites bicyclic monoterpenes cytotoxicity hepatocytes fibroblasts metal ion reduction chelation |
| title | Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures |
| title_full | Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures |
| title_fullStr | Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures |
| title_full_unstemmed | Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures |
| title_short | Metal Ion Reduction, Chelation, and Cytotoxicity of Selected Bicyclic Monoterpenes and Their Binary Mixtures |
| title_sort | metal ion reduction chelation and cytotoxicity of selected bicyclic monoterpenes and their binary mixtures |
| topic | bicyclic monoterpenes cytotoxicity hepatocytes fibroblasts metal ion reduction chelation |
| url | https://www.mdpi.com/2218-1989/15/3/199 |
| work_keys_str_mv | AT karolinawojtunikkulesza metalionreductionchelationandcytotoxicityofselectedbicyclicmonoterpenesandtheirbinarymixtures AT marceladubiel metalionreductionchelationandcytotoxicityofselectedbicyclicmonoterpenesandtheirbinarymixtures AT katarzynaklimek metalionreductionchelationandcytotoxicityofselectedbicyclicmonoterpenesandtheirbinarymixtures |