Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.

Treating macaques with an anti-α4β7 antibody under the umbrella of combination antiretroviral therapy (cART) during early SIV infection can lead to viral remission, with viral loads maintained at < 50 SIV RNA copies/ml after removal of all treatment in a subset of animals. Depletion of CD8+ lymph...

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Main Authors: Chad R Wells, Youfang Cao, David P Durham, Siddappa N Byrareddy, Aftab A Ansari, Nancy H Ruddle, Jeffrey P Townsend, Alison P Galvani, Alan S Perelson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-06-01
Series:PLoS Computational Biology
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189501
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author Chad R Wells
Youfang Cao
David P Durham
Siddappa N Byrareddy
Aftab A Ansari
Nancy H Ruddle
Jeffrey P Townsend
Alison P Galvani
Alan S Perelson
author_facet Chad R Wells
Youfang Cao
David P Durham
Siddappa N Byrareddy
Aftab A Ansari
Nancy H Ruddle
Jeffrey P Townsend
Alison P Galvani
Alan S Perelson
author_sort Chad R Wells
collection DOAJ
description Treating macaques with an anti-α4β7 antibody under the umbrella of combination antiretroviral therapy (cART) during early SIV infection can lead to viral remission, with viral loads maintained at < 50 SIV RNA copies/ml after removal of all treatment in a subset of animals. Depletion of CD8+ lymphocytes in controllers resulted in transient recrudescence of viremia, suggesting that the combination of cART and anti-α4β7 antibody treatment led to a state where ongoing immune responses kept the virus undetectable in the absence of treatment. A previous mathematical model of HIV infection and cART incorporates immune effector cell responses and exhibits the property of two different viral load set-points. While the lower set-point could correspond to the attainment of long-term viral remission, attaining the higher set-point may be the result of viral rebound. Here we expand that model to include possible mechanisms of action of an anti-α4β7 antibody operating in these treated animals. We show that the model can fit the longitudinal viral load data from both IgG control and anti-α4β7 antibody treated macaques, suggesting explanations for the viral control associated with cART and an anti-α4β7 antibody treatment. This effective perturbation to the virus-host interaction can also explain observations in other nonhuman primate experiments in which cART and immunotherapy have led to post-treatment control or resetting of the viral load set-point. Interestingly, because the viral kinetics in the various treated animals differed-some animals exhibited large fluctuations in viral load after cART cessation-the model suggests that anti-α4β7 treatment could act by different primary mechanisms in different animals and still lead to post-treatment viral control. This outcome is nonetheless in accordance with a model with two stable viral load set-points, in which therapy can perturb the system from one set-point to a lower one through different biological mechanisms.
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spelling doaj-art-2f71c47237b549328fef5688e2b25ed32025-08-20T02:18:04ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582021-06-01176e100903110.1371/journal.pcbi.1009031Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.Chad R WellsYoufang CaoDavid P DurhamSiddappa N ByrareddyAftab A AnsariNancy H RuddleJeffrey P TownsendAlison P GalvaniAlan S PerelsonTreating macaques with an anti-α4β7 antibody under the umbrella of combination antiretroviral therapy (cART) during early SIV infection can lead to viral remission, with viral loads maintained at < 50 SIV RNA copies/ml after removal of all treatment in a subset of animals. Depletion of CD8+ lymphocytes in controllers resulted in transient recrudescence of viremia, suggesting that the combination of cART and anti-α4β7 antibody treatment led to a state where ongoing immune responses kept the virus undetectable in the absence of treatment. A previous mathematical model of HIV infection and cART incorporates immune effector cell responses and exhibits the property of two different viral load set-points. While the lower set-point could correspond to the attainment of long-term viral remission, attaining the higher set-point may be the result of viral rebound. Here we expand that model to include possible mechanisms of action of an anti-α4β7 antibody operating in these treated animals. We show that the model can fit the longitudinal viral load data from both IgG control and anti-α4β7 antibody treated macaques, suggesting explanations for the viral control associated with cART and an anti-α4β7 antibody treatment. This effective perturbation to the virus-host interaction can also explain observations in other nonhuman primate experiments in which cART and immunotherapy have led to post-treatment control or resetting of the viral load set-point. Interestingly, because the viral kinetics in the various treated animals differed-some animals exhibited large fluctuations in viral load after cART cessation-the model suggests that anti-α4β7 treatment could act by different primary mechanisms in different animals and still lead to post-treatment viral control. This outcome is nonetheless in accordance with a model with two stable viral load set-points, in which therapy can perturb the system from one set-point to a lower one through different biological mechanisms.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189501
spellingShingle Chad R Wells
Youfang Cao
David P Durham
Siddappa N Byrareddy
Aftab A Ansari
Nancy H Ruddle
Jeffrey P Townsend
Alison P Galvani
Alan S Perelson
Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
PLoS Computational Biology
title Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
title_full Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
title_fullStr Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
title_full_unstemmed Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
title_short Mechanistic basis of post-treatment control of SIV after anti-α4β7 antibody therapy.
title_sort mechanistic basis of post treatment control of siv after anti α4β7 antibody therapy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189501
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