Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice
Objectives: Creatine kinase B (CKB) is the main isoenzyme driving creatine kinase (CK) activity in classical brown adipocytes. However, the specific CK isoenzyme active in beige adipocytes remains unknown. This study aimed to identify the predominant CK isoenzyme expressed and functionally active in...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Molecular Metabolism |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877825001000 |
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| author | Jakub Bunk Mina Ersin Mohammed F. Hussain Bozena Samborska Maria Guerra-Martinez Drishti Soni Lawrence Kazak |
| author_facet | Jakub Bunk Mina Ersin Mohammed F. Hussain Bozena Samborska Maria Guerra-Martinez Drishti Soni Lawrence Kazak |
| author_sort | Jakub Bunk |
| collection | DOAJ |
| description | Objectives: Creatine kinase B (CKB) is the main isoenzyme driving creatine kinase (CK) activity in classical brown adipocytes. However, the specific CK isoenzyme active in beige adipocytes remains unknown. This study aimed to identify the predominant CK isoenzyme expressed and functionally active in beige adipocytes. Methods: CK activity was tracked using D3-creatine tracing in inguinal adipocytes from mice with adipocyte-specific Ckb deletion and their littermate controls, across in vivo and in vitro settings. Results: CKB was essential for CK activity in protein lysates and intact white and beige adipocytes isolated from inguinal fat and drives thermogenesis through the Futile Creatine Cycle. Conclusions: Similar to classical brown adipocytes, CKB is the key functional CK isoenzyme in white and beige adipocytes from the inguinal fat depot. |
| format | Article |
| id | doaj-art-2f719f52c7ce40ff8879ea4abf160b85 |
| institution | Kabale University |
| issn | 2212-8778 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj-art-2f719f52c7ce40ff8879ea4abf160b852025-08-20T03:28:14ZengElsevierMolecular Metabolism2212-87782025-08-019810219310.1016/j.molmet.2025.102193Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in miceJakub Bunk0Mina Ersin1Mohammed F. Hussain2Bozena Samborska3Maria Guerra-Martinez4Drishti Soni5Lawrence Kazak6Rosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, CanadaRosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada; Department of Biochemistry, McGill University, Montreal, QC, H3G 1Y6, Canada; Corresponding author. Rosalind & Morris Goodman Cancer Institute, McGill University, Montreal, QC, H3A 1A3, Canada.Objectives: Creatine kinase B (CKB) is the main isoenzyme driving creatine kinase (CK) activity in classical brown adipocytes. However, the specific CK isoenzyme active in beige adipocytes remains unknown. This study aimed to identify the predominant CK isoenzyme expressed and functionally active in beige adipocytes. Methods: CK activity was tracked using D3-creatine tracing in inguinal adipocytes from mice with adipocyte-specific Ckb deletion and their littermate controls, across in vivo and in vitro settings. Results: CKB was essential for CK activity in protein lysates and intact white and beige adipocytes isolated from inguinal fat and drives thermogenesis through the Futile Creatine Cycle. Conclusions: Similar to classical brown adipocytes, CKB is the key functional CK isoenzyme in white and beige adipocytes from the inguinal fat depot.http://www.sciencedirect.com/science/article/pii/S2212877825001000UCP1-indpendent thermogenesisFutile Creatine CycleCreatine Kinase BTissue-nonspecific alkaline phosphataseBeige adipocytesIsotope tracing |
| spellingShingle | Jakub Bunk Mina Ersin Mohammed F. Hussain Bozena Samborska Maria Guerra-Martinez Drishti Soni Lawrence Kazak Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice Molecular Metabolism UCP1-indpendent thermogenesis Futile Creatine Cycle Creatine Kinase B Tissue-nonspecific alkaline phosphatase Beige adipocytes Isotope tracing |
| title | Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice |
| title_full | Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice |
| title_fullStr | Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice |
| title_full_unstemmed | Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice |
| title_short | Creatine kinase B mediates UCP1-independent beige fat thermogenesis via the futile creatine cycle in mice |
| title_sort | creatine kinase b mediates ucp1 independent beige fat thermogenesis via the futile creatine cycle in mice |
| topic | UCP1-indpendent thermogenesis Futile Creatine Cycle Creatine Kinase B Tissue-nonspecific alkaline phosphatase Beige adipocytes Isotope tracing |
| url | http://www.sciencedirect.com/science/article/pii/S2212877825001000 |
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