Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence

This systematic review aimed to determine how cellulose nanofiber (CNF) enhance the mechanical properties, biocompatibility, and functional versatility of dental materials, based on in vitro evidence. A systematic review protocol was developed according to PRISMA 2020. The literature search was cond...

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Main Authors: Kunhua Yang, Alexander Patera Nugraha, Junduo Chen, Hui Yang, Jingyu Wang, Juan Ramón Vanegas Sáenz, Guang Hong
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Japanese Dental Science Review
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Online Access:http://www.sciencedirect.com/science/article/pii/S1882761625000110
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author Kunhua Yang
Alexander Patera Nugraha
Junduo Chen
Hui Yang
Jingyu Wang
Juan Ramón Vanegas Sáenz
Guang Hong
author_facet Kunhua Yang
Alexander Patera Nugraha
Junduo Chen
Hui Yang
Jingyu Wang
Juan Ramón Vanegas Sáenz
Guang Hong
author_sort Kunhua Yang
collection DOAJ
description This systematic review aimed to determine how cellulose nanofiber (CNF) enhance the mechanical properties, biocompatibility, and functional versatility of dental materials, based on in vitro evidence. A systematic review protocol was developed according to PRISMA 2020. The literature search was conducted using MEDLINE/PubMed, Scopus, Web of Science, Scopus, and Embase for English-language publications with no date restrictions, yielding 180 publications. After removing duplicates, 150 remained, from which 23 were evaluated in full. 17 in vitro studies met the inclusion criteria, of which 13 had a low risk of bias, 4 had a moderate risk, and none had a high risk. Across all included investigations, CNF consistently improved mechanical performance, particularly flexural and compressive strength, while maintaining or enhancing biocompatibility in various cell culture models. CNF’s nanofibrillar structure and modifiable surface chemistry also expanded its functional versatility, enabling applications such as drug encapsulation and targeted antimicrobial delivery. Overall, CNF emerges as a promising biomaterial for modern dentistry, offering superior mechanical reinforcement, favorable cell responses, and wide-ranging functional modifications. Further in vivo research and clinical trials are necessary to confirm its long-term safety and efficacy, thereby facilitating the translation of these in vitro findings into standard dental practice.
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publishDate 2025-12-01
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spelling doaj-art-2f7023e90ba94c23a09cff8abed112782025-08-20T03:20:20ZengElsevierJapanese Dental Science Review1882-76162025-12-016110311110.1016/j.jdsr.2025.05.002Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidenceKunhua Yang0Alexander Patera Nugraha1Junduo Chen2Hui Yang3Jingyu Wang4Juan Ramón Vanegas Sáenz5Guang Hong6Division for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, JapanDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, Japan; Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, IndonesiaDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, JapanDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, JapanState Key Laboratory of Oral & Max Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, ChinaDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, JapanDivision for Globalization Initiative, Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, Japan; Correspondence to: 4-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.This systematic review aimed to determine how cellulose nanofiber (CNF) enhance the mechanical properties, biocompatibility, and functional versatility of dental materials, based on in vitro evidence. A systematic review protocol was developed according to PRISMA 2020. The literature search was conducted using MEDLINE/PubMed, Scopus, Web of Science, Scopus, and Embase for English-language publications with no date restrictions, yielding 180 publications. After removing duplicates, 150 remained, from which 23 were evaluated in full. 17 in vitro studies met the inclusion criteria, of which 13 had a low risk of bias, 4 had a moderate risk, and none had a high risk. Across all included investigations, CNF consistently improved mechanical performance, particularly flexural and compressive strength, while maintaining or enhancing biocompatibility in various cell culture models. CNF’s nanofibrillar structure and modifiable surface chemistry also expanded its functional versatility, enabling applications such as drug encapsulation and targeted antimicrobial delivery. Overall, CNF emerges as a promising biomaterial for modern dentistry, offering superior mechanical reinforcement, favorable cell responses, and wide-ranging functional modifications. Further in vivo research and clinical trials are necessary to confirm its long-term safety and efficacy, thereby facilitating the translation of these in vitro findings into standard dental practice.http://www.sciencedirect.com/science/article/pii/S1882761625000110Cellulose nanofiberDental materialBiocompatibilityMechanical propertiesIn vitro studies
spellingShingle Kunhua Yang
Alexander Patera Nugraha
Junduo Chen
Hui Yang
Jingyu Wang
Juan Ramón Vanegas Sáenz
Guang Hong
Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
Japanese Dental Science Review
Cellulose nanofiber
Dental material
Biocompatibility
Mechanical properties
In vitro studies
title Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
title_full Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
title_fullStr Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
title_full_unstemmed Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
title_short Utilization of cellulose nanofiber in dental applications: A systematic review of in vitro evidence
title_sort utilization of cellulose nanofiber in dental applications a systematic review of in vitro evidence
topic Cellulose nanofiber
Dental material
Biocompatibility
Mechanical properties
In vitro studies
url http://www.sciencedirect.com/science/article/pii/S1882761625000110
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