The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>

(1) Car tyre microplastic particles (TMPs) significantly contribute to global microplastic pollution, with an estimated annual production of 6 million tonnes. However, the impact of TMPs, particularly tyre and road wear particles (TRWPs), resulting from tyre abrasion on the road on terrestrial organ...

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Main Authors: Giorgia Torreggiani, Chiara Manfrin, Anita Giglio, Andrea Dissegna, Cinzia Chiandetti, Paola Giotta, Monia Renzi, Serena Anselmi, Tecla Bentivoglio, Agnieszka Babczyńska, Silvia Battistella, Paolo Edomi, Piero G. Giulianini
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Biomolecules
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Online Access:https://www.mdpi.com/2218-273X/14/12/1640
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author Giorgia Torreggiani
Chiara Manfrin
Anita Giglio
Andrea Dissegna
Cinzia Chiandetti
Paola Giotta
Monia Renzi
Serena Anselmi
Tecla Bentivoglio
Agnieszka Babczyńska
Silvia Battistella
Paolo Edomi
Piero G. Giulianini
author_facet Giorgia Torreggiani
Chiara Manfrin
Anita Giglio
Andrea Dissegna
Cinzia Chiandetti
Paola Giotta
Monia Renzi
Serena Anselmi
Tecla Bentivoglio
Agnieszka Babczyńska
Silvia Battistella
Paolo Edomi
Piero G. Giulianini
author_sort Giorgia Torreggiani
collection DOAJ
description (1) Car tyre microplastic particles (TMPs) significantly contribute to global microplastic pollution, with an estimated annual production of 6 million tonnes. However, the impact of TMPs, particularly tyre and road wear particles (TRWPs), resulting from tyre abrasion on the road on terrestrial organisms, is poorly understood. This study investigated the effects of TMPs and TRWPs on the growth, immune response, behaviour, and cognition of the woodlouse <i>Armadillidium pallasii</i> over 30 days; (2) TMPs and TRWPs were mixed together in the first experiment and provided at different concentrations of 1.25%, 2.5%, 5%, and 10% (<i>w</i>/<i>w</i>), and with soil at 5% and 10% (<i>w</i>/<i>w</i>) concentrations in the second experiment. (3) No differences in survival or immune responses were observed in both experiments. However, isopods exposed to TRWPs showed significant weight gain at lower concentrations but no gain at higher levels. Behavioural tests revealed increased vigilance in TRWP-exposed animals. Micro-FTIR analysis showed that the number of TMPs and TRWPs in the isopods correlated with soil concentrations, and particle size decreased during the experiment. (4) The study highlights the physiological and behavioural effects of TRWPs and the role of detritivorous species in the biofragmentation of TMPs and TRWPs, contributing to the biogeochemical plastic cycle.
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spelling doaj-art-2f6f30a9897c458d85b3bccd8772a9002025-08-20T02:00:54ZengMDPI AGBiomolecules2218-273X2024-12-011412164010.3390/biom14121640The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>Giorgia Torreggiani0Chiara Manfrin1Anita Giglio2Andrea Dissegna3Cinzia Chiandetti4Paola Giotta5Monia Renzi6Serena Anselmi7Tecla Bentivoglio8Agnieszka Babczyńska9Silvia Battistella10Paolo Edomi11Piero G. Giulianini12Department of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Biology, Ecology and Earth Science, University of Calabria, 87036 Rende, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyBioscience Research Center, 58015 Orbetello, ItalyBioscience Research Center, 58015 Orbetello, ItalyFaculty of Natural Sciences, University of Silesia in Katowice, 40-007 Katowice, PolandDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, ItalyDepartment of Life Sciences, University of Trieste, 34127 Trieste, Italy(1) Car tyre microplastic particles (TMPs) significantly contribute to global microplastic pollution, with an estimated annual production of 6 million tonnes. However, the impact of TMPs, particularly tyre and road wear particles (TRWPs), resulting from tyre abrasion on the road on terrestrial organisms, is poorly understood. This study investigated the effects of TMPs and TRWPs on the growth, immune response, behaviour, and cognition of the woodlouse <i>Armadillidium pallasii</i> over 30 days; (2) TMPs and TRWPs were mixed together in the first experiment and provided at different concentrations of 1.25%, 2.5%, 5%, and 10% (<i>w</i>/<i>w</i>), and with soil at 5% and 10% (<i>w</i>/<i>w</i>) concentrations in the second experiment. (3) No differences in survival or immune responses were observed in both experiments. However, isopods exposed to TRWPs showed significant weight gain at lower concentrations but no gain at higher levels. Behavioural tests revealed increased vigilance in TRWP-exposed animals. Micro-FTIR analysis showed that the number of TMPs and TRWPs in the isopods correlated with soil concentrations, and particle size decreased during the experiment. (4) The study highlights the physiological and behavioural effects of TRWPs and the role of detritivorous species in the biofragmentation of TMPs and TRWPs, contributing to the biogeochemical plastic cycle.https://www.mdpi.com/2218-273X/14/12/1640behaviourgrowthhabituationimmune responsesisopodsplastic particle pollution
spellingShingle Giorgia Torreggiani
Chiara Manfrin
Anita Giglio
Andrea Dissegna
Cinzia Chiandetti
Paola Giotta
Monia Renzi
Serena Anselmi
Tecla Bentivoglio
Agnieszka Babczyńska
Silvia Battistella
Paolo Edomi
Piero G. Giulianini
The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
Biomolecules
behaviour
growth
habituation
immune responses
isopods
plastic particle pollution
title The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
title_full The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
title_fullStr The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
title_full_unstemmed The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
title_short The Effect of Tyre and Road Wear Particles on the Terrestrial Isopod <i>Armadillidium pallasii</i>
title_sort effect of tyre and road wear particles on the terrestrial isopod i armadillidium pallasii i
topic behaviour
growth
habituation
immune responses
isopods
plastic particle pollution
url https://www.mdpi.com/2218-273X/14/12/1640
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