Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review

Abstract Background Accumulating evidence demonstrates that miRNAs exhibit enhanced diagnostic sensitivity and specificity compared to the conventional hepatocellular carcinoma (HCC) biomarker alpha-fetoprotein (AFP), particularly showing promising clinical utility in early-stage HCC detection. Howe...

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Main Authors: Shuaiyang Wang, Mei Wu, Bei Xie, Xingyuan Ma, Jing Li, Bin Ma, Linjing Li
Format: Article
Language:English
Published: BMC 2025-06-01
Series:BMC Medical Research Methodology
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Online Access:https://doi.org/10.1186/s12874-025-02610-5
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author Shuaiyang Wang
Mei Wu
Bei Xie
Xingyuan Ma
Jing Li
Bin Ma
Linjing Li
author_facet Shuaiyang Wang
Mei Wu
Bei Xie
Xingyuan Ma
Jing Li
Bin Ma
Linjing Li
author_sort Shuaiyang Wang
collection DOAJ
description Abstract Background Accumulating evidence demonstrates that miRNAs exhibit enhanced diagnostic sensitivity and specificity compared to the conventional hepatocellular carcinoma (HCC) biomarker alpha-fetoprotein (AFP), particularly showing promising clinical utility in early-stage HCC detection. However, insufficient transparency in methodology reporting compromises the validity assessment of these findings and hinders their translational applications. In this study, we investigated the adherence to the STARD criteria in studies investigating the diagnostic accuracy of miRNA for liver cancer. In addition, the quality of reporting was examined to identify factors influencing the quality of reporting. Method A comprehensive search strategy was performed on the PubMed, EMBASE, Cochrane Library, and Web of Science, as of March 30, 2023. Clinical trials investigating the diagnostic value of miRNA for HCC diagnosis were included in the analysis. The eligible studies were checked against adherence to the STARD criteria. Factors determining quality of studies were evaluated through subgroup analyses. All statistical analyses were performed using SPSS (varsion25.0). Results Sixty-two eligible studies, all published between 2010 and 2022, were eventually included in the final analysis. The results revealed a moderate overall adherence to STARD 2015, with an average of 12.1 (52.6%) of the 23 items reported. Subgroup analysis revealed that adherence to the STARD 2015 varied among countries (USA 58.7%;Egypt 46.5%)(p<0.05). Conclusion The quality of studies reporting the diagnostic accuracy of miRNAs in HCC was average and did not increase after the publication of STARD 2015. Our findings rise awareness regarding the need to improve STARD standards, implying that more journals need to include STARD standards for relevant manuscripts. In addition, editorial and peer approval procedures should adopt measures that will aim to improve reporting quality.
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spelling doaj-art-2f6cc0009e2b4f43ad7acc49da2fb1352025-08-20T02:30:42ZengBMCBMC Medical Research Methodology1471-22882025-06-0125111110.1186/s12874-025-02610-5Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic reviewShuaiyang Wang0Mei Wu1Bei Xie2Xingyuan Ma3Jing Li4Bin Ma5Linjing Li6Department of Clinical Laboratory Center, The Second Hospital of Lanzhou UniversityEvidence-Based Medicine Centre, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Clinical Laboratory Center, The Second Hospital of Lanzhou UniversityDepartment of Clinical Laboratory Center, The Second Hospital of Lanzhou UniversityEvidence-Based Medicine Centre, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Clinical Laboratory Center, The Second Hospital of Lanzhou UniversityAbstract Background Accumulating evidence demonstrates that miRNAs exhibit enhanced diagnostic sensitivity and specificity compared to the conventional hepatocellular carcinoma (HCC) biomarker alpha-fetoprotein (AFP), particularly showing promising clinical utility in early-stage HCC detection. However, insufficient transparency in methodology reporting compromises the validity assessment of these findings and hinders their translational applications. In this study, we investigated the adherence to the STARD criteria in studies investigating the diagnostic accuracy of miRNA for liver cancer. In addition, the quality of reporting was examined to identify factors influencing the quality of reporting. Method A comprehensive search strategy was performed on the PubMed, EMBASE, Cochrane Library, and Web of Science, as of March 30, 2023. Clinical trials investigating the diagnostic value of miRNA for HCC diagnosis were included in the analysis. The eligible studies were checked against adherence to the STARD criteria. Factors determining quality of studies were evaluated through subgroup analyses. All statistical analyses were performed using SPSS (varsion25.0). Results Sixty-two eligible studies, all published between 2010 and 2022, were eventually included in the final analysis. The results revealed a moderate overall adherence to STARD 2015, with an average of 12.1 (52.6%) of the 23 items reported. Subgroup analysis revealed that adherence to the STARD 2015 varied among countries (USA 58.7%;Egypt 46.5%)(p<0.05). Conclusion The quality of studies reporting the diagnostic accuracy of miRNAs in HCC was average and did not increase after the publication of STARD 2015. Our findings rise awareness regarding the need to improve STARD standards, implying that more journals need to include STARD standards for relevant manuscripts. In addition, editorial and peer approval procedures should adopt measures that will aim to improve reporting quality.https://doi.org/10.1186/s12874-025-02610-5MiRNAHCCSTARDReportDiagnosisChecklist
spellingShingle Shuaiyang Wang
Mei Wu
Bei Xie
Xingyuan Ma
Jing Li
Bin Ma
Linjing Li
Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
BMC Medical Research Methodology
MiRNA
HCC
STARD
Report
Diagnosis
Checklist
title Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
title_full Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
title_fullStr Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
title_full_unstemmed Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
title_short Adherence to STARD guidelines in miRNA diagnostic accuracy studies for hepatocellular carcinoma: a systematic review
title_sort adherence to stard guidelines in mirna diagnostic accuracy studies for hepatocellular carcinoma a systematic review
topic MiRNA
HCC
STARD
Report
Diagnosis
Checklist
url https://doi.org/10.1186/s12874-025-02610-5
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