Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies
<b>Objectives</b>: This analysis aims to provide evidence supporting the feasibility of clinical application of self-assembling peptides for skin wound healing. <b>Methods</b>: This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-A...
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MDPI AG
2025-04-01
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| author | Jiaju Lu Liuting Chen Zeyue Sun Zhimou Yang |
| author_facet | Jiaju Lu Liuting Chen Zeyue Sun Zhimou Yang |
| author_sort | Jiaju Lu |
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| description | <b>Objectives</b>: This analysis aims to provide evidence supporting the feasibility of clinical application of self-assembling peptides for skin wound healing. <b>Methods</b>: This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. PubMed, Web of Science, and Cochrane Library were searched (up to June 17, 2024). The primary outcome, wound closure rate at 7 and 14 days post-injury, was pooled using a random-effects meta-analysis. The risk of bias (ROB) assessment and meta-analysis were performed using the Systematic Review Centre for Laboratory animal Experimentation (SYRCLE)’s ROB tool for animal studies and RevMan software. <b>Results</b>: A total of 502 unique records were identified from our search, with 12 experimental animal studies meeting the prespecified inclusion criteria (n = 272 animals). The RADA16 interventions promoted wound closure rate compared to controls (saline or no treatment group) in both diabetic and non-diabetic animal models (Mean Difference (MD) = 11.25, 95% Confidence Interval (CI): 5.73 to 16.78, <i>p</i> < 0.0001; MD = 9.48, 95% CI: 4.75 to 14.22, <i>p</i> < 0.0001 at 7 and 14 days post-injury, respectively). Healing was further enhanced using RADA16-based functional self-assembling peptides compared to RADA16 group in both diabetic and non-diabetic animal models (MD = 27.25, 95% CI: 22.68 to 31.83, <i>p</i> < 0.00001; MD = 29.11, 95% CI: 24.30 to 33.91, <i>p</i> < 0.00001 at 7 and 14 days after injury, respectively). The ROB was uncertain for most studies due to insufficient reporting. <b>Conclusions</b>: RADA16-based self-assembling peptides, particularly those modified with functional peptide motifs, represent a promising treatment for non-diabetic and diabetic wounds in pre-clinical studies, and translation to the clinical domain appears warranted. |
| format | Article |
| id | doaj-art-2f66c36e83d4448a8061c5464bbe4f62 |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-2f66c36e83d4448a8061c5464bbe4f622025-08-20T03:13:58ZengMDPI AGPharmaceuticals1424-82472025-04-0118452610.3390/ph18040526Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal StudiesJiaju Lu0Liuting Chen1Zeyue Sun2Zhimou Yang3Key Laboratory of Bioactive Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, ChinaSchool of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaSchool of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, ChinaKey Laboratory of Bioactive Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, China<b>Objectives</b>: This analysis aims to provide evidence supporting the feasibility of clinical application of self-assembling peptides for skin wound healing. <b>Methods</b>: This review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. PubMed, Web of Science, and Cochrane Library were searched (up to June 17, 2024). The primary outcome, wound closure rate at 7 and 14 days post-injury, was pooled using a random-effects meta-analysis. The risk of bias (ROB) assessment and meta-analysis were performed using the Systematic Review Centre for Laboratory animal Experimentation (SYRCLE)’s ROB tool for animal studies and RevMan software. <b>Results</b>: A total of 502 unique records were identified from our search, with 12 experimental animal studies meeting the prespecified inclusion criteria (n = 272 animals). The RADA16 interventions promoted wound closure rate compared to controls (saline or no treatment group) in both diabetic and non-diabetic animal models (Mean Difference (MD) = 11.25, 95% Confidence Interval (CI): 5.73 to 16.78, <i>p</i> < 0.0001; MD = 9.48, 95% CI: 4.75 to 14.22, <i>p</i> < 0.0001 at 7 and 14 days post-injury, respectively). Healing was further enhanced using RADA16-based functional self-assembling peptides compared to RADA16 group in both diabetic and non-diabetic animal models (MD = 27.25, 95% CI: 22.68 to 31.83, <i>p</i> < 0.00001; MD = 29.11, 95% CI: 24.30 to 33.91, <i>p</i> < 0.00001 at 7 and 14 days after injury, respectively). The ROB was uncertain for most studies due to insufficient reporting. <b>Conclusions</b>: RADA16-based self-assembling peptides, particularly those modified with functional peptide motifs, represent a promising treatment for non-diabetic and diabetic wounds in pre-clinical studies, and translation to the clinical domain appears warranted.https://www.mdpi.com/1424-8247/18/4/526wound healingRADA16self-assembling peptidesmeta-analysis |
| spellingShingle | Jiaju Lu Liuting Chen Zeyue Sun Zhimou Yang Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies Pharmaceuticals wound healing RADA16 self-assembling peptides meta-analysis |
| title | Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies |
| title_full | Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies |
| title_fullStr | Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies |
| title_full_unstemmed | Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies |
| title_short | Efficacy of RADA16-Based Self-Assembling Peptides on Wound Healing: A Meta-Analysis of Preclinical Animal Studies |
| title_sort | efficacy of rada16 based self assembling peptides on wound healing a meta analysis of preclinical animal studies |
| topic | wound healing RADA16 self-assembling peptides meta-analysis |
| url | https://www.mdpi.com/1424-8247/18/4/526 |
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