Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand

Abstract Astrovirus (AstV) is an RNA virus that causes gastroenteritis in humans and various mammal species, including dogs and cats. In this study, we conducted a survey of canine and feline astroviruses in domestic dogs and cats in Bangkok and its vicinity in Thailand. From January 2022 to Decembe...

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Main Authors: Yu Nandi Thaw, Kamonpan Charoenkul, Chanakarn Nasamran, Ekkapat Chamsai, Waleemas Jairak, Eaint Min Phyu, Hnin Wai Phyu, Supassama Chaiyawong, Alongkorn Amonsin
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Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-03037-0
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author Yu Nandi Thaw
Kamonpan Charoenkul
Chanakarn Nasamran
Ekkapat Chamsai
Waleemas Jairak
Eaint Min Phyu
Hnin Wai Phyu
Supassama Chaiyawong
Alongkorn Amonsin
author_facet Yu Nandi Thaw
Kamonpan Charoenkul
Chanakarn Nasamran
Ekkapat Chamsai
Waleemas Jairak
Eaint Min Phyu
Hnin Wai Phyu
Supassama Chaiyawong
Alongkorn Amonsin
author_sort Yu Nandi Thaw
collection DOAJ
description Abstract Astrovirus (AstV) is an RNA virus that causes gastroenteritis in humans and various mammal species, including dogs and cats. In this study, we conducted a survey of canine and feline astroviruses in domestic dogs and cats in Bangkok and its vicinity in Thailand. From January 2022 to December 2023, we collected a total of 1,498 rectal swab samples from domestic dogs (n = 862) and cats (n = 636) at private small animal hospitals in Bangkok, Nonthaburi, and Samut Prakran, Thailand. The positive rate for canine astrovirus (CaAstV) was 4.2% (36/862), while that of feline astrovirus (FeAstV) in cats was 10.8% (69/636). We performed complete genome sequencing of Thai-CaAstVs (n = 11) and Thai-FeAstVs (n = 12). Phylogenetic analysis revealed that Thai-CaAstVs were clustered into lineages 1, 3, and 4, while Thai-FeAstVs were grouped within FeAstV group 1. This study identifies novel CaAstV lineages in domestic dogs and represents the first molecular detection of FeAstV in cats in Thailand. Our findings provide detailed information on the genetic characteristics of CaAstVs and FeAstVs currently circulating in Thailand, highlighting challenges for potential diagnostics and future control strategies.
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spelling doaj-art-2f59b384b5ee40bdacebd2aca7d5faa22025-08-20T03:22:09ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-03037-0Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in ThailandYu Nandi Thaw0Kamonpan Charoenkul1Chanakarn Nasamran2Ekkapat Chamsai3Waleemas Jairak4Eaint Min Phyu5Hnin Wai Phyu6Supassama Chaiyawong7Alongkorn Amonsin8Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityCenter of Excellence for Emerging and Re-emerging Infectious Diseases in Animals, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityDepartment of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn UniversityAbstract Astrovirus (AstV) is an RNA virus that causes gastroenteritis in humans and various mammal species, including dogs and cats. In this study, we conducted a survey of canine and feline astroviruses in domestic dogs and cats in Bangkok and its vicinity in Thailand. From January 2022 to December 2023, we collected a total of 1,498 rectal swab samples from domestic dogs (n = 862) and cats (n = 636) at private small animal hospitals in Bangkok, Nonthaburi, and Samut Prakran, Thailand. The positive rate for canine astrovirus (CaAstV) was 4.2% (36/862), while that of feline astrovirus (FeAstV) in cats was 10.8% (69/636). We performed complete genome sequencing of Thai-CaAstVs (n = 11) and Thai-FeAstVs (n = 12). Phylogenetic analysis revealed that Thai-CaAstVs were clustered into lineages 1, 3, and 4, while Thai-FeAstVs were grouped within FeAstV group 1. This study identifies novel CaAstV lineages in domestic dogs and represents the first molecular detection of FeAstV in cats in Thailand. Our findings provide detailed information on the genetic characteristics of CaAstVs and FeAstVs currently circulating in Thailand, highlighting challenges for potential diagnostics and future control strategies.https://doi.org/10.1038/s41598-025-03037-0Canine and feline astrovirusCatDogGenetic characterizationThailand
spellingShingle Yu Nandi Thaw
Kamonpan Charoenkul
Chanakarn Nasamran
Ekkapat Chamsai
Waleemas Jairak
Eaint Min Phyu
Hnin Wai Phyu
Supassama Chaiyawong
Alongkorn Amonsin
Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
Scientific Reports
Canine and feline astrovirus
Cat
Dog
Genetic characterization
Thailand
title Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
title_full Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
title_fullStr Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
title_full_unstemmed Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
title_short Monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in Thailand
title_sort monitoring and characterization of canine and feline astroviruses in domestic dogs and cats in thailand
topic Canine and feline astrovirus
Cat
Dog
Genetic characterization
Thailand
url https://doi.org/10.1038/s41598-025-03037-0
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