Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy
Myocardial ischemia/reperfusion (I/R) injury seriously threats the health and life of patients with ischemia heart disease. Herein, we probed the potential influence of myosin 1b (myo1b) on hypoxia/reoxygenation- (H/R-) stimulated cardiomyocyte H9c2 cell apoptosis and autophagy. After H/R stimulatio...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2022/5187304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849699117176979456 |
|---|---|
| author | Jing Xu Jin Huang Xiaojie He Mingshuang Hu Shan Su Ping Liu |
| author_facet | Jing Xu Jin Huang Xiaojie He Mingshuang Hu Shan Su Ping Liu |
| author_sort | Jing Xu |
| collection | DOAJ |
| description | Myocardial ischemia/reperfusion (I/R) injury seriously threats the health and life of patients with ischemia heart disease. Herein, we probed the potential influence of myosin 1b (myo1b) on hypoxia/reoxygenation- (H/R-) stimulated cardiomyocyte H9c2 cell apoptosis and autophagy. After H/R stimulation, the myo1b mRNA level in H9c2 cells was tested via qRT-PCR. Myo1b overexpression plasmid (OE-myo1b) and small interfering RNA (siRNA) targeting myo1b (si-myo1b) were transfected into H9c2 cells to alter myo1b expression in H9c2 cells. Following H/R stimulation and/or OE-myo1b (or si-myo1b) transfection, H9c2 cell apoptosis, proliferation, and autophagy were detected, respectively. We found that H/R stimulation reduced the mRNA level of myo1b in H9c2 cells and resulted in H9c2 cell apoptosis, proliferation inhibition, and autophagy. Overexpression of myo1b reversed the H/R-resulted H9c2 cell apoptosis, proliferation inhibition, and autophagy. Silence of myo1b had opposite effects, which promoted H9c2 cell apoptosis, reduced cell proliferation, and accelerated cell autophagy. Taken together, Myo1b took part in the modulation of H/R-stimulated cardiomyocyte apoptosis and autophagy, which might be serve as a potential endogenous target for prevention and therapy of I/R injury. |
| format | Article |
| id | doaj-art-2f54e45d75974292b084fb4c56fc3d62 |
| institution | DOAJ |
| issn | 2210-7185 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-2f54e45d75974292b084fb4c56fc3d622025-08-20T03:18:42ZengWileyAnalytical Cellular Pathology2210-71852022-01-01202210.1155/2022/5187304Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and AutophagyJing Xu0Jin Huang1Xiaojie He2Mingshuang Hu3Shan Su4Ping Liu5Clinical Nursing Teaching and Research SectionClinical Nursing Teaching and Research SectionNephrology LaboratoryClinical Nursing Teaching and Research SectionClinical Nursing Teaching and Research SectionClinical Nursing Teaching and Research SectionMyocardial ischemia/reperfusion (I/R) injury seriously threats the health and life of patients with ischemia heart disease. Herein, we probed the potential influence of myosin 1b (myo1b) on hypoxia/reoxygenation- (H/R-) stimulated cardiomyocyte H9c2 cell apoptosis and autophagy. After H/R stimulation, the myo1b mRNA level in H9c2 cells was tested via qRT-PCR. Myo1b overexpression plasmid (OE-myo1b) and small interfering RNA (siRNA) targeting myo1b (si-myo1b) were transfected into H9c2 cells to alter myo1b expression in H9c2 cells. Following H/R stimulation and/or OE-myo1b (or si-myo1b) transfection, H9c2 cell apoptosis, proliferation, and autophagy were detected, respectively. We found that H/R stimulation reduced the mRNA level of myo1b in H9c2 cells and resulted in H9c2 cell apoptosis, proliferation inhibition, and autophagy. Overexpression of myo1b reversed the H/R-resulted H9c2 cell apoptosis, proliferation inhibition, and autophagy. Silence of myo1b had opposite effects, which promoted H9c2 cell apoptosis, reduced cell proliferation, and accelerated cell autophagy. Taken together, Myo1b took part in the modulation of H/R-stimulated cardiomyocyte apoptosis and autophagy, which might be serve as a potential endogenous target for prevention and therapy of I/R injury.http://dx.doi.org/10.1155/2022/5187304 |
| spellingShingle | Jing Xu Jin Huang Xiaojie He Mingshuang Hu Shan Su Ping Liu Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy Analytical Cellular Pathology |
| title | Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy |
| title_full | Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy |
| title_fullStr | Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy |
| title_full_unstemmed | Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy |
| title_short | Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy |
| title_sort | myosin 1b participated in the modulation of hypoxia reoxygenation caused h9c2 cell apoptosis and autophagy |
| url | http://dx.doi.org/10.1155/2022/5187304 |
| work_keys_str_mv | AT jingxu myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy AT jinhuang myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy AT xiaojiehe myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy AT mingshuanghu myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy AT shansu myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy AT pingliu myosin1bparticipatedinthemodulationofhypoxiareoxygenationcausedh9c2cellapoptosisandautophagy |