A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i>
Based on transcriptome and proteome sequencing technologies, this study aims to preliminarily reveal the molecular mechanisms of growth and development and related metabolic regulation in <i>Morchella sextelat</i>. A total of 42.31 GB of Clean Data was acquired from the transcriptome seq...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/3/192 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849342666844667904 |
|---|---|
| author | Weilin Feng Zier Guo Qunli Jin Fei Xu Yingyue Shen Tingting Song Mei Wang Jun Zhang Lijun Fan Xianbin Huang Weiming Cai |
| author_facet | Weilin Feng Zier Guo Qunli Jin Fei Xu Yingyue Shen Tingting Song Mei Wang Jun Zhang Lijun Fan Xianbin Huang Weiming Cai |
| author_sort | Weilin Feng |
| collection | DOAJ |
| description | Based on transcriptome and proteome sequencing technologies, this study aims to preliminarily reveal the molecular mechanisms of growth and development and related metabolic regulation in <i>Morchella sextelat</i>. A total of 42.31 GB of Clean Data was acquired from the transcriptome sequencing (RNA-seq) of six samples in two development phases (<i>n</i> = 3) of <i>M. sextelata.</i> In the young phase (YP) and harvest phase (HP), there were 2887 differentially expressed genes (DEGs), including 1910 up-regulated genes and 977 down-regulated genes. In YP and HP, there were 987 differentially expressed proteins (DEPs), including 417 up-regulated ones and 570 down-regulated ones. Based on GO and KEGG analysis, significant differences in the transcriptomes and proteins in metabolic pathways are disclosed. Glycometabolism, especially starch, saccharose, and polysaccharide metabolism, plays a crucial role in the growth of <i>M. sextelata.</i> In addition, expression changes in the genes related to selenium metabolism are here recognized. These research results not only offer strong support for further exploration of the biological significance and functional differences of <i>M. sextelata</i>, but are also conducive to discovering key genes and understanding their regulation network during growth. |
| format | Article |
| id | doaj-art-2f535b8d3a654ca5ad9346fe024a90a4 |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-2f535b8d3a654ca5ad9346fe024a90a42025-08-20T03:43:16ZengMDPI AGJournal of Fungi2309-608X2025-03-0111319210.3390/jof11030192A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i>Weilin Feng0Zier Guo1Qunli Jin2Fei Xu3Yingyue Shen4Tingting Song5Mei Wang6Jun Zhang7Lijun Fan8Xianbin Huang9Weiming Cai10Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threas to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaBased on transcriptome and proteome sequencing technologies, this study aims to preliminarily reveal the molecular mechanisms of growth and development and related metabolic regulation in <i>Morchella sextelat</i>. A total of 42.31 GB of Clean Data was acquired from the transcriptome sequencing (RNA-seq) of six samples in two development phases (<i>n</i> = 3) of <i>M. sextelata.</i> In the young phase (YP) and harvest phase (HP), there were 2887 differentially expressed genes (DEGs), including 1910 up-regulated genes and 977 down-regulated genes. In YP and HP, there were 987 differentially expressed proteins (DEPs), including 417 up-regulated ones and 570 down-regulated ones. Based on GO and KEGG analysis, significant differences in the transcriptomes and proteins in metabolic pathways are disclosed. Glycometabolism, especially starch, saccharose, and polysaccharide metabolism, plays a crucial role in the growth of <i>M. sextelata.</i> In addition, expression changes in the genes related to selenium metabolism are here recognized. These research results not only offer strong support for further exploration of the biological significance and functional differences of <i>M. sextelata</i>, but are also conducive to discovering key genes and understanding their regulation network during growth.https://www.mdpi.com/2309-608X/11/3/192RNA-seqselenium<i>Morchella sextelata</i>glycometabolism |
| spellingShingle | Weilin Feng Zier Guo Qunli Jin Fei Xu Yingyue Shen Tingting Song Mei Wang Jun Zhang Lijun Fan Xianbin Huang Weiming Cai A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> Journal of Fungi RNA-seq selenium <i>Morchella sextelata</i> glycometabolism |
| title | A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> |
| title_full | A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> |
| title_fullStr | A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> |
| title_full_unstemmed | A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> |
| title_short | A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i> |
| title_sort | preliminary exploration of transcriptome and proteomic changes during the young and harvest periods in i morchella sextelata i |
| topic | RNA-seq selenium <i>Morchella sextelata</i> glycometabolism |
| url | https://www.mdpi.com/2309-608X/11/3/192 |
| work_keys_str_mv | AT weilinfeng apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT zierguo apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT qunlijin apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT feixu apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT yingyueshen apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT tingtingsong apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT meiwang apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT junzhang apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT lijunfan apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT xianbinhuang apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT weimingcai apreliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT weilinfeng preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT zierguo preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT qunlijin preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT feixu preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT yingyueshen preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT tingtingsong preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT meiwang preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT junzhang preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT lijunfan preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT xianbinhuang preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai AT weimingcai preliminaryexplorationoftranscriptomeandproteomicchangesduringtheyoungandharvestperiodsinimorchellasextelatai |