Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors

Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors

ABSTRACT We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screening included the previously reported KIAA0319L, TM9SF2, and RNF121, along with a cluster of genes involved in glycan biogenesis, Golgi...

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Main Authors: Xiujuan Zhang, Siyuan Hao, Zehua Feng, Kang Ning, Cagla Aksu Kuz, Shane McFarlin, Donovan Richart, Fang Cheng, Ander Zhang-Chen, Richenda McFarlane, Ziying Yan, Jianming Qiu
Format: Article
Language:English
Published: American Society for Microbiology 2025-01-01
Series:mBio
Subjects:
rAAV
SLC35A1
transduction
intracellular trafficking
nuclear import
Online Access:https://journals.asm.org/doi/10.1128/mbio.03268-24
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