The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy

Abstract Background Collagen type III glomerulopathy (CG) is a rare idiopathic nephropathy characterized by the massive deposition of dense, fractured, curved, band-like fibers in the mesangial and subendothelial regions. Ultrastructural pathological examination confirms that these deposits are comp...

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Main Authors: Fangcheng Zhang, Qi Shen, Xiaoling Sun, Xiao Long, Dan Cheng
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Nephrology
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Online Access:https://doi.org/10.1186/s12882-025-04315-9
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author Fangcheng Zhang
Qi Shen
Xiaoling Sun
Xiao Long
Dan Cheng
author_facet Fangcheng Zhang
Qi Shen
Xiaoling Sun
Xiao Long
Dan Cheng
author_sort Fangcheng Zhang
collection DOAJ
description Abstract Background Collagen type III glomerulopathy (CG) is a rare idiopathic nephropathy characterized by the massive deposition of dense, fractured, curved, band-like fibers in the mesangial and subendothelial regions. Ultrastructural pathological examination confirms that these deposits are composed of type III collagen. Here, we report two rare cases of CG: Patient I was superimposed with membranous nephropathy (MN), and Patient II was superimposed with diabetic nephropathy (DN), both confirmed by transmission electron microscopy (EM). Case presentation Both patients presented with bilateral lower extremity edema of unknown etiology. Patient I was admitted to the hospital multiple times due to persistent proteinuria. The phospholipase A2 receptor (PLA2R) test was positive, but there was no response to steroid treatment. Patient II has a 10-year history of hypertension and fasting hyperglycemia. Renal biopsies revealed segmental aggregation of homogeneous substances beneath the mesangium and endothelial cells. To obtain a definitive diagnosis, biopsy specimens were transferred to our department for electron microscopic evaluation. Conclusion This study highlights the ultrastructural characteristics of CG and emphasizes the indispensable role of electron microscopy in the diagnosis of CG, particularly when coexisting with other glomerular diseases. The Early EM examination in renal biopsies is crucial for a clear diagnosis and prognosis prediction.
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institution Kabale University
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publishDate 2025-07-01
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series BMC Nephrology
spelling doaj-art-2f4a16e720124f1ca2bcc5fda8168a472025-08-20T03:42:48ZengBMCBMC Nephrology1471-23692025-07-012611610.1186/s12882-025-04315-9The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathyFangcheng Zhang0Qi Shen1Xiaoling Sun2Xiao Long3Dan Cheng4Department of Pathology, Center of Ultrapathology, Renmin Hospital of Wuhan UniversityDepartment of Operating Room, Zhongnan Hospital of Wuhan UniversityDepartment of Pathology, Center of Ultrapathology, Renmin Hospital of Wuhan UniversityDepartment of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan UniversityAbstract Background Collagen type III glomerulopathy (CG) is a rare idiopathic nephropathy characterized by the massive deposition of dense, fractured, curved, band-like fibers in the mesangial and subendothelial regions. Ultrastructural pathological examination confirms that these deposits are composed of type III collagen. Here, we report two rare cases of CG: Patient I was superimposed with membranous nephropathy (MN), and Patient II was superimposed with diabetic nephropathy (DN), both confirmed by transmission electron microscopy (EM). Case presentation Both patients presented with bilateral lower extremity edema of unknown etiology. Patient I was admitted to the hospital multiple times due to persistent proteinuria. The phospholipase A2 receptor (PLA2R) test was positive, but there was no response to steroid treatment. Patient II has a 10-year history of hypertension and fasting hyperglycemia. Renal biopsies revealed segmental aggregation of homogeneous substances beneath the mesangium and endothelial cells. To obtain a definitive diagnosis, biopsy specimens were transferred to our department for electron microscopic evaluation. Conclusion This study highlights the ultrastructural characteristics of CG and emphasizes the indispensable role of electron microscopy in the diagnosis of CG, particularly when coexisting with other glomerular diseases. The Early EM examination in renal biopsies is crucial for a clear diagnosis and prognosis prediction.https://doi.org/10.1186/s12882-025-04315-9Collagen type III glomerulopathyElectron microscopyDiagnosisMembranous nephropathyDiabetic nephropathy
spellingShingle Fangcheng Zhang
Qi Shen
Xiaoling Sun
Xiao Long
Dan Cheng
The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
BMC Nephrology
Collagen type III glomerulopathy
Electron microscopy
Diagnosis
Membranous nephropathy
Diabetic nephropathy
title The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
title_full The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
title_fullStr The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
title_full_unstemmed The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
title_short The essential role of electron microscopy in the diagnosis of collagen type III glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
title_sort essential role of electron microscopy in the diagnosis of collagen type iii glomerulopathy superimposed with membranous nephropathy or diabetic nephropathy
topic Collagen type III glomerulopathy
Electron microscopy
Diagnosis
Membranous nephropathy
Diabetic nephropathy
url https://doi.org/10.1186/s12882-025-04315-9
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