IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY
Background: The CTX-M gene in ESBLs-producing Klebsiella pneumoniae is associated with virulence factors and encodes for antibiotic resistance. There are currently 128 different varieties of CTX-M, which gene can hydrolyze beta-lactam medicines like cefotaxime. Purpose: Identify the CTX-M gene in Kl...
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Universitas Airlangga
2023-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/40135 |
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| author | Anang Kurniawan Joko Setyono Dwi Utami Anjarawati |
| author_facet | Anang Kurniawan Joko Setyono Dwi Utami Anjarawati |
| author_sort | Anang Kurniawan |
| collection | DOAJ |
| description | Background: The CTX-M gene in ESBLs-producing Klebsiella pneumoniae is associated with virulence factors and encodes for antibiotic resistance. There are currently 128 different varieties of CTX-M, which gene can hydrolyze beta-lactam medicines like cefotaxime. Purpose: Identify the CTX-M gene in Klebsiella pneumoniae from patients with Urinary Tract Infections (UTI) at a private hospital in Banyumas Region of Central Java, Indonesia. Method: Cross-sectional survey method was employed in this study. The subjects of the study comprised 40 patients with UTI admitted to a private hospital in Banyumas Region, Central Java, Indonesia. Using a direct molecular approach called Polymerase Chain Reaction (PCR) directed against the CTX-M gene, DNA was isolated from isolates cultured in CHROMagarTM ESBLs obtained from catheter urine samples. Result: A total of 4 out of 40 urine samples (10%) tested positive for ESBL-producing Klebsiella pneumoniae. The PCR was performed to detect the CTX-M gene in 100% of the strains. Conclusion: These findings suggest the presence of CTX-M gene found in ESBLs-producing Klebsiella pneumoniae in individuals with urinary tract infections in a private hospital in Purwokerto, Banyumas regency, Central Java, Indonesia. This information can be used to assess antibiotic administration practices. |
| format | Article |
| id | doaj-art-2f468e0af30f4b378aa1bcd12efdc386 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-2f468e0af30f4b378aa1bcd12efdc3862025-02-03T07:11:13ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-11-0172909410.20473/jvhs.V7.I2.2023.90-9438206IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDYAnang Kurniawan0https://orcid.org/0000-0001-8803-9695Joko Setyono1https://orcid.org/0000-0002-9512-203XDwi Utami Anjarawati2https://orcid.org/0000-0001-8394-2543Biomedical Program, Faculty of Medicine, Jenderal Soedirman University, Central JavaDepartment of Biochemistry, Faculty of Medicine, Jenderal Soedirman University, Central JavaDepartement of Microbiology, Faculty of Medicine, Jenderal Soedirman University, Purwokerto, Central Java Background: The CTX-M gene in ESBLs-producing Klebsiella pneumoniae is associated with virulence factors and encodes for antibiotic resistance. There are currently 128 different varieties of CTX-M, which gene can hydrolyze beta-lactam medicines like cefotaxime. Purpose: Identify the CTX-M gene in Klebsiella pneumoniae from patients with Urinary Tract Infections (UTI) at a private hospital in Banyumas Region of Central Java, Indonesia. Method: Cross-sectional survey method was employed in this study. The subjects of the study comprised 40 patients with UTI admitted to a private hospital in Banyumas Region, Central Java, Indonesia. Using a direct molecular approach called Polymerase Chain Reaction (PCR) directed against the CTX-M gene, DNA was isolated from isolates cultured in CHROMagarTM ESBLs obtained from catheter urine samples. Result: A total of 4 out of 40 urine samples (10%) tested positive for ESBL-producing Klebsiella pneumoniae. The PCR was performed to detect the CTX-M gene in 100% of the strains. Conclusion: These findings suggest the presence of CTX-M gene found in ESBLs-producing Klebsiella pneumoniae in individuals with urinary tract infections in a private hospital in Purwokerto, Banyumas regency, Central Java, Indonesia. This information can be used to assess antibiotic administration practices.https://e-journal.unair.ac.id/JVHS/article/view/40135ctx-mutiklebsiella pneumoniaeesblsantibiotic resistance |
| spellingShingle | Anang Kurniawan Joko Setyono Dwi Utami Anjarawati IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY Journal of Vocational Health Studies ctx-m uti klebsiella pneumoniae esbls antibiotic resistance |
| title | IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY |
| title_full | IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY |
| title_fullStr | IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY |
| title_full_unstemmed | IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY |
| title_short | IDENTIFICATION OF THE CTX-M GENE IN KLEBSIELLA PNEUMONIAE PRODUCING ESBLS IN UTI PATIENTS AT A PRIVATE HOSPITAL IN PURWOKERTO, BANYUMAS REGENCY, CENTRAL JAVA - CROSS SECTIONAL STUDY |
| title_sort | identification of the ctx m gene in klebsiella pneumoniae producing esbls in uti patients at a private hospital in purwokerto banyumas regency central java cross sectional study |
| topic | ctx-m uti klebsiella pneumoniae esbls antibiotic resistance |
| url | https://e-journal.unair.ac.id/JVHS/article/view/40135 |
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