A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus
Abstract This retrospective study analyzed serological and molecular diagnostic data from 7,170 EBV-infected patients treated at the Clinical Laboratory Center of Lanzhou University Second Hospital between 2018 and 2024. The aim was to evaluate the clinical utility of combining EBV DNA quantificatio...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-025-11204-x |
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| author | Hongwei Gao Weiguo Wang Xin Ma Anan Zhang Yunyan Pan |
| author_facet | Hongwei Gao Weiguo Wang Xin Ma Anan Zhang Yunyan Pan |
| author_sort | Hongwei Gao |
| collection | DOAJ |
| description | Abstract This retrospective study analyzed serological and molecular diagnostic data from 7,170 EBV-infected patients treated at the Clinical Laboratory Center of Lanzhou University Second Hospital between 2018 and 2024. The aim was to evaluate the clinical utility of combining EBV DNA quantification via qPCR with antibody testing (VCA-IgM, VCA-IgG, EA-IgM, EBNA-IgG, and VCA-IgA) using ELISA. Significant age-related variations were observed across DNA replication groups, with the high viral load (1 × 10⁵ copies/mL) predominantly detected in adolescents (median age: 13 years). VCA-IgM demonstrated the strongest association with DNA replication levels (OR = 0.168, p < 0.001), yet 45.2% (14/31) of high viral load(1 × 10⁵ copies/mL) cases were VCA-IgM negative, underscoring the limitations of relying solely on serology in immunocompromised populations. Our findings emphasize the necessity of integrating molecular and serological testing for accurate EBV diagnosis, particularly in resource-limited settings. |
| format | Article |
| id | doaj-art-2f353552c94243ae91963a3d2202afd8 |
| institution | Kabale University |
| issn | 1471-2334 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj-art-2f353552c94243ae91963a3d2202afd82025-08-20T03:37:23ZengBMCBMC Infectious Diseases1471-23342025-07-012511810.1186/s12879-025-11204-xA single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virusHongwei Gao0Weiguo Wang1Xin Ma2Anan Zhang3Yunyan Pan4Laboratory Medicine Center, The Second Hospital & Clinical Medical School, Lanzhou UniversityLaboratory Medicine Center, The Second Hospital & Clinical Medical School, Lanzhou UniversityLaboratory Medicine Center, The Second Hospital & Clinical Medical School, Lanzhou UniversityLaboratory Medicine Center, The Second Hospital & Clinical Medical School, Lanzhou UniversityLaboratory Medicine Center, The Second Hospital & Clinical Medical School, Lanzhou UniversityAbstract This retrospective study analyzed serological and molecular diagnostic data from 7,170 EBV-infected patients treated at the Clinical Laboratory Center of Lanzhou University Second Hospital between 2018 and 2024. The aim was to evaluate the clinical utility of combining EBV DNA quantification via qPCR with antibody testing (VCA-IgM, VCA-IgG, EA-IgM, EBNA-IgG, and VCA-IgA) using ELISA. Significant age-related variations were observed across DNA replication groups, with the high viral load (1 × 10⁵ copies/mL) predominantly detected in adolescents (median age: 13 years). VCA-IgM demonstrated the strongest association with DNA replication levels (OR = 0.168, p < 0.001), yet 45.2% (14/31) of high viral load(1 × 10⁵ copies/mL) cases were VCA-IgM negative, underscoring the limitations of relying solely on serology in immunocompromised populations. Our findings emphasize the necessity of integrating molecular and serological testing for accurate EBV diagnosis, particularly in resource-limited settings.https://doi.org/10.1186/s12879-025-11204-xEBVSerological assaysMolecular diagnosticsDNA replicationDiagnostic implications |
| spellingShingle | Hongwei Gao Weiguo Wang Xin Ma Anan Zhang Yunyan Pan A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus BMC Infectious Diseases EBV Serological assays Molecular diagnostics DNA replication Diagnostic implications |
| title | A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus |
| title_full | A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus |
| title_fullStr | A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus |
| title_full_unstemmed | A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus |
| title_short | A single-center retrospective analysis of serological and molecular findings in patients infected with Epstein-Barr virus |
| title_sort | single center retrospective analysis of serological and molecular findings in patients infected with epstein barr virus |
| topic | EBV Serological assays Molecular diagnostics DNA replication Diagnostic implications |
| url | https://doi.org/10.1186/s12879-025-11204-x |
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