Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy
Diabetic nephropathy is a major cause of chronic kidney disease and end-stage kidney disease. However, so little is known about alterations of the glycopatterns in urine with the development of diabetic nephropathy. Presently, we interrogated glycopatterns in urine specimens using a lectin microarra...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2017/5728087 |
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| author | Hanyu Zhu Moyan Liu Hanjie Yu Xiawei Liu Yaogang Zhong Jian Shu Xinle Fu Guangyan Cai Xiangmei Chen Wenjia Geng Xiaoli Yang Minghui Wu Zheng Li Dong Zhang |
| author_facet | Hanyu Zhu Moyan Liu Hanjie Yu Xiawei Liu Yaogang Zhong Jian Shu Xinle Fu Guangyan Cai Xiangmei Chen Wenjia Geng Xiaoli Yang Minghui Wu Zheng Li Dong Zhang |
| author_sort | Hanyu Zhu |
| collection | DOAJ |
| description | Diabetic nephropathy is a major cause of chronic kidney disease and end-stage kidney disease. However, so little is known about alterations of the glycopatterns in urine with the development of diabetic nephropathy. Presently, we interrogated glycopatterns in urine specimens using a lectin microarray. The results showed that expression levels of Siaα2-6Gal/GalNAc recognized by SNA exhibited significantly increased tendency with the development of diabetic nephropathy; moreover, SNA blotting indicated glycoproteins (90 kDa, 70 kDa, and 40 kDa) in urine may contribute to this alteration. Furthermore, the glycopatterns of (GlcNAc)2–4 recognized by STL exhibited difference between diabetic and nondiabetic nephropathy. The results of urinary protein microarray fabricated by another 48 urine specimens also indicated (GlcNAc)2–4 is a potential indictor to differentiate the patients with diabetic nephropathy from nondiabetic nephropathy. Furtherly, STL blotting showed that the 50 kDa glycoproteins were correlated with this alteration. In conclusion, our data provide pivotal information to monitor the development of diabetic nephropathy and distinguish between diabetic nephropathy and nondiabetic renal disease based on precise alterations of glycopatterns in urinary proteins, but further studies are needed in this regard. |
| format | Article |
| id | doaj-art-2f2e40b0339f484c9e63c0d35fb35e1a |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-2f2e40b0339f484c9e63c0d35fb35e1a2025-02-03T06:06:36ZengWileyJournal of Diabetes Research2314-67452314-67532017-01-01201710.1155/2017/57280875728087Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic NephropathyHanyu Zhu0Moyan Liu1Hanjie Yu2Xiawei Liu3Yaogang Zhong4Jian Shu5Xinle Fu6Guangyan Cai7Xiangmei Chen8Wenjia Geng9Xiaoli Yang10Minghui Wu11Zheng Li12Dong Zhang13Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDepartment of Nephrology, General Hospital of Jinan Military Command, Jinan, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaLaboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an, ChinaDepartment of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center of Kidney Diseases, Beijing Key Laboratory of Kidney Disease, Beijing, ChinaDiabetic nephropathy is a major cause of chronic kidney disease and end-stage kidney disease. However, so little is known about alterations of the glycopatterns in urine with the development of diabetic nephropathy. Presently, we interrogated glycopatterns in urine specimens using a lectin microarray. The results showed that expression levels of Siaα2-6Gal/GalNAc recognized by SNA exhibited significantly increased tendency with the development of diabetic nephropathy; moreover, SNA blotting indicated glycoproteins (90 kDa, 70 kDa, and 40 kDa) in urine may contribute to this alteration. Furthermore, the glycopatterns of (GlcNAc)2–4 recognized by STL exhibited difference between diabetic and nondiabetic nephropathy. The results of urinary protein microarray fabricated by another 48 urine specimens also indicated (GlcNAc)2–4 is a potential indictor to differentiate the patients with diabetic nephropathy from nondiabetic nephropathy. Furtherly, STL blotting showed that the 50 kDa glycoproteins were correlated with this alteration. In conclusion, our data provide pivotal information to monitor the development of diabetic nephropathy and distinguish between diabetic nephropathy and nondiabetic renal disease based on precise alterations of glycopatterns in urinary proteins, but further studies are needed in this regard.http://dx.doi.org/10.1155/2017/5728087 |
| spellingShingle | Hanyu Zhu Moyan Liu Hanjie Yu Xiawei Liu Yaogang Zhong Jian Shu Xinle Fu Guangyan Cai Xiangmei Chen Wenjia Geng Xiaoli Yang Minghui Wu Zheng Li Dong Zhang Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy Journal of Diabetes Research |
| title | Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy |
| title_full | Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy |
| title_fullStr | Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy |
| title_full_unstemmed | Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy |
| title_short | Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy |
| title_sort | glycopatterns of urinary protein as new potential diagnosis indicators for diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2017/5728087 |
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