A novel evaluation of genetic polymorphism in BCG adenitis
Bacillus Calmette-Guerin (BCG) is a live attenuated vaccine which has been used to prevent tuberculosis, according to the World Health Organization (WHO) recommendation in parts of the world with an incidence of tuberculosis infection more than 1%. The incidence of BCG adverse reactions differs bet...
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Hacettepe University Institute of Child Health
2019-06-01
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| Series: | The Turkish Journal of Pediatrics |
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| Online Access: | https://turkjpediatr.org/article/view/722 |
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| author | Maryam Hassanzad Poupak Farnia Sepideh Darougar Ali Akbar Velayati |
| author_facet | Maryam Hassanzad Poupak Farnia Sepideh Darougar Ali Akbar Velayati |
| author_sort | Maryam Hassanzad |
| collection | DOAJ |
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Bacillus Calmette-Guerin (BCG) is a live attenuated vaccine which has been used to prevent tuberculosis, according to the World Health Organization (WHO) recommendation in parts of the world with an incidence of tuberculosis infection more than 1%. The incidence of BCG adverse reactions differs between regions with regional lymphadenitis as the most common presentation. The aim of this study was to detect the impact of polymorphisms causing BCG lymphadenitis in children receiving BCG vaccination at birth. Eight healthy infants with BCG adenitis from 4 to 12 months old were enrolled. All these patients underwent a thorough physical examination, abdominopelvic ultrasound evaluation to detect distant lymphadenopathies and immunodeficiency screening tests for any possible underlying immunodeficiency disorders. Then genotyping for known mutations was performed using restriction fragments length polymorphism (PCR-RFLP) assays. Sequencing was performed for IL-12 Rβ1, IFN-ϒ receptor 1, IL-10, TNF-α and P2X7. The mean age of onset of the adenitis was 6.5 months. TNF-857, IL-12Rβ1 705, IL-10 1082, and IFN-ϒ- 56 single nucleotide polymorphisms (SNPs) were common in the children studied. The most frequent polymorphism found in the patients with BCG adenitis except one, was the P2X7 -762 polymorphism. To conclude, these polymorphisms are more common in some ethnic populations but not others and make the genetic basis of immunity to BCG strains and the occurrence of post-BCG lymphadenitis in otherwise healthy children.
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| format | Article |
| id | doaj-art-2f29c056029b4e5cb813723faee729a2 |
| institution | DOAJ |
| issn | 0041-4301 2791-6421 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | Hacettepe University Institute of Child Health |
| record_format | Article |
| series | The Turkish Journal of Pediatrics |
| spelling | doaj-art-2f29c056029b4e5cb813723faee729a22025-08-20T02:55:21ZengHacettepe University Institute of Child HealthThe Turkish Journal of Pediatrics0041-43012791-64212019-06-0161310.24953/turkjped.2019.03.026A novel evaluation of genetic polymorphism in BCG adenitisMaryam Hassanzad0Poupak FarniaSepideh Darougar1Ali Akbar Velayati2Pediatric Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran.Department of Pediatrics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran. Bacillus Calmette-Guerin (BCG) is a live attenuated vaccine which has been used to prevent tuberculosis, according to the World Health Organization (WHO) recommendation in parts of the world with an incidence of tuberculosis infection more than 1%. The incidence of BCG adverse reactions differs between regions with regional lymphadenitis as the most common presentation. The aim of this study was to detect the impact of polymorphisms causing BCG lymphadenitis in children receiving BCG vaccination at birth. Eight healthy infants with BCG adenitis from 4 to 12 months old were enrolled. All these patients underwent a thorough physical examination, abdominopelvic ultrasound evaluation to detect distant lymphadenopathies and immunodeficiency screening tests for any possible underlying immunodeficiency disorders. Then genotyping for known mutations was performed using restriction fragments length polymorphism (PCR-RFLP) assays. Sequencing was performed for IL-12 Rβ1, IFN-ϒ receptor 1, IL-10, TNF-α and P2X7. The mean age of onset of the adenitis was 6.5 months. TNF-857, IL-12Rβ1 705, IL-10 1082, and IFN-ϒ- 56 single nucleotide polymorphisms (SNPs) were common in the children studied. The most frequent polymorphism found in the patients with BCG adenitis except one, was the P2X7 -762 polymorphism. To conclude, these polymorphisms are more common in some ethnic populations but not others and make the genetic basis of immunity to BCG strains and the occurrence of post-BCG lymphadenitis in otherwise healthy children. https://turkjpediatr.org/article/view/722BCGadenitisgeneticimmunizationpolymorphism |
| spellingShingle | Maryam Hassanzad Poupak Farnia Sepideh Darougar Ali Akbar Velayati A novel evaluation of genetic polymorphism in BCG adenitis The Turkish Journal of Pediatrics BCG adenitis genetic immunization polymorphism |
| title | A novel evaluation of genetic polymorphism in BCG adenitis |
| title_full | A novel evaluation of genetic polymorphism in BCG adenitis |
| title_fullStr | A novel evaluation of genetic polymorphism in BCG adenitis |
| title_full_unstemmed | A novel evaluation of genetic polymorphism in BCG adenitis |
| title_short | A novel evaluation of genetic polymorphism in BCG adenitis |
| title_sort | novel evaluation of genetic polymorphism in bcg adenitis |
| topic | BCG adenitis genetic immunization polymorphism |
| url | https://turkjpediatr.org/article/view/722 |
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