Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis
Background Dysregulated circular RNAs (circRNAs) are involved in osteoarthritis (OA) progression.Objective We aimed to explore the effect of hsa_circ_0044719 (circTRIM25) on the ferroptosis of chondrocytes.Methods Chondrocytes were treated with interleukin (IL)-1β to generate cell model. Cellular be...
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Taylor & Francis Group
2024-12-01
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| Series: | Autoimmunity |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/08916934.2024.2361749 |
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| author | Chunlei He Zhaogan Zeng Yadong Yang Shanshan Ye Qiang Wu Xunzhi Liu Chenghong Liu Wanhui Zeng Sheng Liu |
| author_facet | Chunlei He Zhaogan Zeng Yadong Yang Shanshan Ye Qiang Wu Xunzhi Liu Chenghong Liu Wanhui Zeng Sheng Liu |
| author_sort | Chunlei He |
| collection | DOAJ |
| description | Background Dysregulated circular RNAs (circRNAs) are involved in osteoarthritis (OA) progression.Objective We aimed to explore the effect of hsa_circ_0044719 (circTRIM25) on the ferroptosis of chondrocytes.Methods Chondrocytes were treated with interleukin (IL)-1β to generate cell model. Cellular behaviours were measured using cell counting kit-8, enzyme-linked immunosorbent assay, relevant kits, propidium iodide staining, and immunofluorescence assay. Quantitative real-time polymerase chain reaction was performed to examine the expression of circTRIM25, miR-138-5p, and cAMP responsive element binding protein 1 (CREB1), and their interactions were assessed using luciferase reporter analysis and RNA pull-down assay.Results CircTRIM25 was upregulated in OA tissues and IL-1β-stimulated chondrocytes. Knockdown of circTRIM25 facilitated the viability and suppressed ferroptosis and inflammation of IL-1β-induced cells. CircTRIM25 served as a sponge of miR-138-5p, which directly targets CREB1. Downregulation of miR-138-5p abrogated the effect induced by knockdown of circTRIM25. Furthermore, enforced CREB1 reversed the miR-138-5p induced effect. Moreover, knockdown of circTRIM25 attenuated cartilage injury in vivo.Conclusion Silencing of circTRIM25 inhibited ferroptosis of chondrocytes via the miR-138-5p/CREB axis and thus attenuated OA progression. |
| format | Article |
| id | doaj-art-2f1dd11b5b77478bbd7a4c746dccaf5b |
| institution | OA Journals |
| issn | 0891-6934 1607-842X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Autoimmunity |
| spelling | doaj-art-2f1dd11b5b77478bbd7a4c746dccaf5b2025-08-20T02:38:31ZengTaylor & Francis GroupAutoimmunity0891-69341607-842X2024-12-0157110.1080/08916934.2024.2361749Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritisChunlei He0Zhaogan Zeng1Yadong Yang2Shanshan Ye3Qiang Wu4Xunzhi Liu5Chenghong Liu6Wanhui Zeng7Sheng Liu8Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaGannan Medical University, Ganzhou, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaGannan Medical University, Ganzhou, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaDepartment of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, ChinaBackground Dysregulated circular RNAs (circRNAs) are involved in osteoarthritis (OA) progression.Objective We aimed to explore the effect of hsa_circ_0044719 (circTRIM25) on the ferroptosis of chondrocytes.Methods Chondrocytes were treated with interleukin (IL)-1β to generate cell model. Cellular behaviours were measured using cell counting kit-8, enzyme-linked immunosorbent assay, relevant kits, propidium iodide staining, and immunofluorescence assay. Quantitative real-time polymerase chain reaction was performed to examine the expression of circTRIM25, miR-138-5p, and cAMP responsive element binding protein 1 (CREB1), and their interactions were assessed using luciferase reporter analysis and RNA pull-down assay.Results CircTRIM25 was upregulated in OA tissues and IL-1β-stimulated chondrocytes. Knockdown of circTRIM25 facilitated the viability and suppressed ferroptosis and inflammation of IL-1β-induced cells. CircTRIM25 served as a sponge of miR-138-5p, which directly targets CREB1. Downregulation of miR-138-5p abrogated the effect induced by knockdown of circTRIM25. Furthermore, enforced CREB1 reversed the miR-138-5p induced effect. Moreover, knockdown of circTRIM25 attenuated cartilage injury in vivo.Conclusion Silencing of circTRIM25 inhibited ferroptosis of chondrocytes via the miR-138-5p/CREB axis and thus attenuated OA progression.https://www.tandfonline.com/doi/10.1080/08916934.2024.2361749OsteoarthritiscircTRIM25ferroptosismiR-138a-5pcartilage regeneration |
| spellingShingle | Chunlei He Zhaogan Zeng Yadong Yang Shanshan Ye Qiang Wu Xunzhi Liu Chenghong Liu Wanhui Zeng Sheng Liu Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis Autoimmunity Osteoarthritis circTRIM25 ferroptosis miR-138a-5p cartilage regeneration |
| title | Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis |
| title_full | Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis |
| title_fullStr | Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis |
| title_full_unstemmed | Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis |
| title_short | Silencing of CircTRIM25/miR-138-5p/CREB1 axis promotes chondrogenesis in osteoarthritis |
| title_sort | silencing of circtrim25 mir 138 5p creb1 axis promotes chondrogenesis in osteoarthritis |
| topic | Osteoarthritis circTRIM25 ferroptosis miR-138a-5p cartilage regeneration |
| url | https://www.tandfonline.com/doi/10.1080/08916934.2024.2361749 |
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