Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium
The extraction technology of sterol was confirmed by ethanol reflux and saponification in this study. The orthogonal test was employed to assess the impact of extraction time, solid–liquid ratio, ethanol concentration and extraction temperature on the yield of sterol extraction. Hyperlipidemia model...
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MDPI AG
2025-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/14/2498 |
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| author | Chao Wang Yuan Liu Yuting Duan Haiping Lin |
| author_facet | Chao Wang Yuan Liu Yuting Duan Haiping Lin |
| author_sort | Chao Wang |
| collection | DOAJ |
| description | The extraction technology of sterol was confirmed by ethanol reflux and saponification in this study. The orthogonal test was employed to assess the impact of extraction time, solid–liquid ratio, ethanol concentration and extraction temperature on the yield of sterol extraction. Hyperlipidemia model mice were established by feeding a high-fat and -sugar diet, and different doses of sterol extracts were given to the mice by gavages. The optimal extraction conditions were identified as an extraction time of 80 min, a solid–liquid ratio of 1:10, an ethanol concentration of 95%, and an extraction temperature of 90 °C, resulting in a sterol concentration of 1.16 mg/g. Compared with the high-fat model group, the high-dose group significantly reduced body weight by 17.2%, liver weight by 30.9%, and serum low density lipoprotein cholesterol by 20.0% (<i>p</i> < 0.05), while serum total cholesterol (5.59 ± 0.48 vs. 5.68 ± 0.64 mmol/L) and high-density lipoprotein cholesterol (0.98 ± 0.05 vs. 0.93 ± 0.03 mmol/L) showed no significant changes compared to the model group. |
| format | Article |
| id | doaj-art-2f1d5d8ecad540d0a96eb3cd8c0bff56 |
| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-2f1d5d8ecad540d0a96eb3cd8c0bff562025-08-20T02:45:56ZengMDPI AGFoods2304-81582025-07-011414249810.3390/foods14142498Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing SclerotiumChao Wang0Yuan Liu1Yuting Duan2Haiping Lin3College of Food and Health, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Food and Health, Zhejiang A&F University, Hangzhou 311300, ChinaCollege of Food and Health, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, ChinaThe extraction technology of sterol was confirmed by ethanol reflux and saponification in this study. The orthogonal test was employed to assess the impact of extraction time, solid–liquid ratio, ethanol concentration and extraction temperature on the yield of sterol extraction. Hyperlipidemia model mice were established by feeding a high-fat and -sugar diet, and different doses of sterol extracts were given to the mice by gavages. The optimal extraction conditions were identified as an extraction time of 80 min, a solid–liquid ratio of 1:10, an ethanol concentration of 95%, and an extraction temperature of 90 °C, resulting in a sterol concentration of 1.16 mg/g. Compared with the high-fat model group, the high-dose group significantly reduced body weight by 17.2%, liver weight by 30.9%, and serum low density lipoprotein cholesterol by 20.0% (<i>p</i> < 0.05), while serum total cholesterol (5.59 ± 0.48 vs. 5.68 ± 0.64 mmol/L) and high-density lipoprotein cholesterol (0.98 ± 0.05 vs. 0.93 ± 0.03 mmol/L) showed no significant changes compared to the model group.https://www.mdpi.com/2304-8158/14/14/2498<i>Pleurotus tuber-regium</i>(Fr.) sing sclerotiumsterolextraction technologyhyperlipidemia |
| spellingShingle | Chao Wang Yuan Liu Yuting Duan Haiping Lin Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium Foods <i>Pleurotus tuber-regium</i>(Fr.) sing sclerotium sterol extraction technology hyperlipidemia |
| title | Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium |
| title_full | Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium |
| title_fullStr | Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium |
| title_full_unstemmed | Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium |
| title_short | Exploration of Hypolipidemic Effects of Sterols from <i>Pleurotus tuber-regium</i>(Fr.) Sing Sclerotium |
| title_sort | exploration of hypolipidemic effects of sterols from i pleurotus tuber regium i fr sing sclerotium |
| topic | <i>Pleurotus tuber-regium</i>(Fr.) sing sclerotium sterol extraction technology hyperlipidemia |
| url | https://www.mdpi.com/2304-8158/14/14/2498 |
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