Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human plate...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7108458 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850106213135548416 |
|---|---|
| author | Lauren H. Mangum Shanmugasundaram Natesan Randolph Stone Nicole L. Wrice David A. Larson Kyle F. Florell Barbara A. Christy Maryanne C. Herzig Andrew P. Cap Robert J. Christy |
| author_facet | Lauren H. Mangum Shanmugasundaram Natesan Randolph Stone Nicole L. Wrice David A. Larson Kyle F. Florell Barbara A. Christy Maryanne C. Herzig Andrew P. Cap Robert J. Christy |
| author_sort | Lauren H. Mangum |
| collection | DOAJ |
| description | Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95–99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p<0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes. |
| format | Article |
| id | doaj-art-2efc84ba5e444494bcb05ab48091609f |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-2efc84ba5e444494bcb05ab48091609f2025-08-20T02:38:52ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/71084587108458Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem CellsLauren H. Mangum0Shanmugasundaram Natesan1Randolph Stone2Nicole L. Wrice3David A. Larson4Kyle F. Florell5Barbara A. Christy6Maryanne C. Herzig7Andrew P. Cap8Robert J. Christy9Combat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACoagulation and Blood Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACoagulation and Blood Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACoagulation and Blood Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USACombat Trauma and Burn Injury Research, US Army Institute of Surgical Research, San Antonio Military Medical Center, JBSA Ft Sam Houston, San Antonio, TX, USAStem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95–99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p<0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.http://dx.doi.org/10.1155/2017/7108458 |
| spellingShingle | Lauren H. Mangum Shanmugasundaram Natesan Randolph Stone Nicole L. Wrice David A. Larson Kyle F. Florell Barbara A. Christy Maryanne C. Herzig Andrew P. Cap Robert J. Christy Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells Stem Cells International |
| title | Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells |
| title_full | Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells |
| title_fullStr | Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells |
| title_full_unstemmed | Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells |
| title_short | Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells |
| title_sort | tissue source and cell expansion condition influence phenotypic changes of adipose derived stem cells |
| url | http://dx.doi.org/10.1155/2017/7108458 |
| work_keys_str_mv | AT laurenhmangum tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT shanmugasundaramnatesan tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT randolphstone tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT nicolelwrice tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT davidalarson tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT kylefflorell tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT barbaraachristy tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT maryannecherzig tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT andrewpcap tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells AT robertjchristy tissuesourceandcellexpansionconditioninfluencephenotypicchangesofadiposederivedstemcells |