RTN family and its response to abiotic stress in Oryza sativa

Abstract Abiotic stresses induced by climate change and soil pollution cause significant crop yield loss. Identifying stress-resistant genes for genetic improvement is urgent and necessary. Reticulon (RTN, also called RTNLB in plants) is a widely distributed endoplasmic reticulum-associated protein...

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Main Authors: Gao-Hui Cao, Xin-Li Guo, Yue-Jiao Yang, Bin Xie, Lu-Lu Tang
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-97261-3
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author Gao-Hui Cao
Xin-Li Guo
Yue-Jiao Yang
Bin Xie
Lu-Lu Tang
author_facet Gao-Hui Cao
Xin-Li Guo
Yue-Jiao Yang
Bin Xie
Lu-Lu Tang
author_sort Gao-Hui Cao
collection DOAJ
description Abstract Abiotic stresses induced by climate change and soil pollution cause significant crop yield loss. Identifying stress-resistant genes for genetic improvement is urgent and necessary. Reticulon (RTN, also called RTNLB in plants) is a widely distributed endoplasmic reticulum-associated protein in eukaryotic cells, playing a crucial role in stress response activities, including autophagy, endoplasmic reticulum stress, and osmotic regulation. However, the diversity of RTNLBs in plants has hindered comprehensive analysis. This study identified 20 OsRTNLBs from Oryza sativa through a comprehensive whole-genome analysis. Detailed information on their chromosomal localization, protein motifs, phylogenetic relationships, and gene collinearity has been provided. The effects of five major abiotic stresses—cold, heat, heavy metals, salt, and drought—on rice seedling development have been assessed. The expression levels of RTNLBs under these abiotic stresses show significant changes, with an overall increase in transcription levels under simulated drought stress and a decrease under the other four stresses. Three OsRTNLBs (OsRTNLB4, OsRTNLB8 and OsRTNLB9) have been identified as key proteins contributing to abiotic stress.
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publishDate 2025-04-01
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spelling doaj-art-2ef6b967571d4dedbcfebbf648e697ef2025-08-20T03:18:38ZengNature PortfolioScientific Reports2045-23222025-04-0115111610.1038/s41598-025-97261-3RTN family and its response to abiotic stress in Oryza sativaGao-Hui Cao0Xin-Li Guo1Yue-Jiao Yang2Bin Xie3Lu-Lu Tang4Department of Cell Biology, School of Life Sciences, Central South UniversityDepartment of Cell Biology, School of Life Sciences, Central South UniversityDepartment of Cell Biology, School of Life Sciences, Central South UniversityState Key Laboratory of Hybrid Rice (Wuhan University)Department of Cell Biology, School of Life Sciences, Central South UniversityAbstract Abiotic stresses induced by climate change and soil pollution cause significant crop yield loss. Identifying stress-resistant genes for genetic improvement is urgent and necessary. Reticulon (RTN, also called RTNLB in plants) is a widely distributed endoplasmic reticulum-associated protein in eukaryotic cells, playing a crucial role in stress response activities, including autophagy, endoplasmic reticulum stress, and osmotic regulation. However, the diversity of RTNLBs in plants has hindered comprehensive analysis. This study identified 20 OsRTNLBs from Oryza sativa through a comprehensive whole-genome analysis. Detailed information on their chromosomal localization, protein motifs, phylogenetic relationships, and gene collinearity has been provided. The effects of five major abiotic stresses—cold, heat, heavy metals, salt, and drought—on rice seedling development have been assessed. The expression levels of RTNLBs under these abiotic stresses show significant changes, with an overall increase in transcription levels under simulated drought stress and a decrease under the other four stresses. Three OsRTNLBs (OsRTNLB4, OsRTNLB8 and OsRTNLB9) have been identified as key proteins contributing to abiotic stress.https://doi.org/10.1038/s41598-025-97261-3Reticulon (RTN, RTNLB) familyOryza sativaAbiotic stress response
spellingShingle Gao-Hui Cao
Xin-Li Guo
Yue-Jiao Yang
Bin Xie
Lu-Lu Tang
RTN family and its response to abiotic stress in Oryza sativa
Scientific Reports
Reticulon (RTN, RTNLB) family
Oryza sativa
Abiotic stress response
title RTN family and its response to abiotic stress in Oryza sativa
title_full RTN family and its response to abiotic stress in Oryza sativa
title_fullStr RTN family and its response to abiotic stress in Oryza sativa
title_full_unstemmed RTN family and its response to abiotic stress in Oryza sativa
title_short RTN family and its response to abiotic stress in Oryza sativa
title_sort rtn family and its response to abiotic stress in oryza sativa
topic Reticulon (RTN, RTNLB) family
Oryza sativa
Abiotic stress response
url https://doi.org/10.1038/s41598-025-97261-3
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