Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study
Introduction: Mouthwashes enhance oral hygiene by reducing plaque and refreshing breath. Probiotic mouthwashes containing Lactobacillus bacteria offer antimicrobial benefits and reduce oral pathogens. Aim: To develop a mouthwash containing Lactobacillus bacteria, Vitamin C, and lavender oil, and to...
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JCDR Research and Publications Private Limited
2025-05-01
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| Online Access: | https://jcdr.net/articles/PDF/20958/75046_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(VD_SS_SL)_redo_PFA(IS)_PB(VD_IS)_PN(IS).pdf |
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| author | R Bharath PT Priyangha |
| author_facet | R Bharath PT Priyangha |
| author_sort | R Bharath |
| collection | DOAJ |
| description | Introduction: Mouthwashes enhance oral hygiene by reducing plaque and refreshing breath. Probiotic mouthwashes containing Lactobacillus bacteria offer antimicrobial benefits and reduce oral pathogens.
Aim: To develop a mouthwash containing Lactobacillus bacteria, Vitamin C, and lavender oil, and to evaluate its in-vitro properties, such as antimicrobial, anti-inflammatory, and cytotoxic properties.
Materials and Methods: This is an in-vitro study conducted in Green Lab, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India between August 2024 and September 2024. To prepare the mouthwash, distilled water was heated to 40-45°C for ingredient dissolution of ingredients. Vitamin C was added in powdered form to achieve a final concentration of 0.1-1%, and glycerin (5%) was added. Lavender oil (0.1-0.5%) was incorporated with Polysorbate 20 as an emulsifier. The mixture was cooled below 40°C before adding Lactobacillus plantarum MTCC 5690. The pH was adjusted to 5.5-7.0, followed by adding xylitol and sodium benzoate as a preservative, and the final product was filtered for purity. The mouthwash was evaluated in-vitro for its antimicrobial, anti-inflammatory, and cytotoxic properties. International Business Machine (IBM) Statistical Packages of Social Sciences (SPSS) Statistics version 21.0 was used to perform one-way Analysis of Variance (ANOVA), with a p-value of less than 0.05 considered statistically significant.
Results: The antimicrobial activity assay revealed that the mouthwash showed the largest inhibition zones for Streptococcus mutans, with a mean of 17±1.5 mm for High Concentration (HC) formulation and 16±1.5 mm for Low Concentration (LC) formulation. This confirming that the HC mouthwash produced larger inhibition zones than the LC formulation. The results were statistically significant (p<0.05) for Staphylococcus aureus, Streptococcus mutans, and Escherichia coli, indicating meaningful differences among different concentration groups for these microorganisms. The anti-inflammatory assay revealed that at a concentration of 50 μL/mL, the mouthwash significantly reduced protein denaturation, showing an absorbance of 3.9 at 570 nm. For the cytotoxic effects, the highest values were observed at 50 μL/mL, with an absorbance of 0.22, demonstrating a dose-dependent increase. Fluorescence microscopy showed increased cell viability at 50 μL/mL (Grade 0), indicating the highest level of protection for the cells.
Conclusion: In conclusion, the formulated mouthwash that combines probiotics, Vitamin C, and lavender oil shows effective antimicrobial and anti-inflammatory effects, thus presenting a promising alternative for enhancing periodontal health. |
| format | Article |
| id | doaj-art-2ef66ff6cb1a41fb9fb4c727fbbcc3be |
| institution | DOAJ |
| issn | 2249-782X 0973-709X |
| language | English |
| publishDate | 2025-05-01 |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj-art-2ef66ff6cb1a41fb9fb4c727fbbcc3be2025-08-20T03:09:16ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-05-01195ZC12ZC1710.7860/JCDR/2025/75046.20958Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro StudyR Bharath0PT Priyangha1Dentist, Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India.Senior Lecturer, Department of Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India.Introduction: Mouthwashes enhance oral hygiene by reducing plaque and refreshing breath. Probiotic mouthwashes containing Lactobacillus bacteria offer antimicrobial benefits and reduce oral pathogens. Aim: To develop a mouthwash containing Lactobacillus bacteria, Vitamin C, and lavender oil, and to evaluate its in-vitro properties, such as antimicrobial, anti-inflammatory, and cytotoxic properties. Materials and Methods: This is an in-vitro study conducted in Green Lab, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India between August 2024 and September 2024. To prepare the mouthwash, distilled water was heated to 40-45°C for ingredient dissolution of ingredients. Vitamin C was added in powdered form to achieve a final concentration of 0.1-1%, and glycerin (5%) was added. Lavender oil (0.1-0.5%) was incorporated with Polysorbate 20 as an emulsifier. The mixture was cooled below 40°C before adding Lactobacillus plantarum MTCC 5690. The pH was adjusted to 5.5-7.0, followed by adding xylitol and sodium benzoate as a preservative, and the final product was filtered for purity. The mouthwash was evaluated in-vitro for its antimicrobial, anti-inflammatory, and cytotoxic properties. International Business Machine (IBM) Statistical Packages of Social Sciences (SPSS) Statistics version 21.0 was used to perform one-way Analysis of Variance (ANOVA), with a p-value of less than 0.05 considered statistically significant. Results: The antimicrobial activity assay revealed that the mouthwash showed the largest inhibition zones for Streptococcus mutans, with a mean of 17±1.5 mm for High Concentration (HC) formulation and 16±1.5 mm for Low Concentration (LC) formulation. This confirming that the HC mouthwash produced larger inhibition zones than the LC formulation. The results were statistically significant (p<0.05) for Staphylococcus aureus, Streptococcus mutans, and Escherichia coli, indicating meaningful differences among different concentration groups for these microorganisms. The anti-inflammatory assay revealed that at a concentration of 50 μL/mL, the mouthwash significantly reduced protein denaturation, showing an absorbance of 3.9 at 570 nm. For the cytotoxic effects, the highest values were observed at 50 μL/mL, with an absorbance of 0.22, demonstrating a dose-dependent increase. Fluorescence microscopy showed increased cell viability at 50 μL/mL (Grade 0), indicating the highest level of protection for the cells. Conclusion: In conclusion, the formulated mouthwash that combines probiotics, Vitamin C, and lavender oil shows effective antimicrobial and anti-inflammatory effects, thus presenting a promising alternative for enhancing periodontal health.https://jcdr.net/articles/PDF/20958/75046_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(VD_SS_SL)_redo_PFA(IS)_PB(VD_IS)_PN(IS).pdfdental plaqueoral hygienetherapeutics |
| spellingShingle | R Bharath PT Priyangha Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study Journal of Clinical and Diagnostic Research dental plaque oral hygiene therapeutics |
| title | Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study |
| title_full | Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study |
| title_fullStr | Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study |
| title_full_unstemmed | Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study |
| title_short | Synergistic Formulation of Lactobacillus Probiotics, Vitamin C and Lavender Oil in Mouthwash: An In-vitro Study |
| title_sort | synergistic formulation of lactobacillus probiotics vitamin c and lavender oil in mouthwash an in vitro study |
| topic | dental plaque oral hygiene therapeutics |
| url | https://jcdr.net/articles/PDF/20958/75046_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(VD_SS_SL)_redo_PFA(IS)_PB(VD_IS)_PN(IS).pdf |
| work_keys_str_mv | AT rbharath synergisticformulationoflactobacillusprobioticsvitamincandlavenderoilinmouthwashaninvitrostudy AT ptpriyangha synergisticformulationoflactobacillusprobioticsvitamincandlavenderoilinmouthwashaninvitrostudy |