Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture
Abstract This study introduces groundbreaking cryopreservation, being the first to successfully cryopreserve the juvenile stage of a marine organism, the largest marine organism ever cryopreserved. By optimizing feeding strategies, equilibration times, and cryoprotectant concentrations, we extended...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-11439-3 |
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| author | A. Lago J. Troncoso E. Paredes |
| author_facet | A. Lago J. Troncoso E. Paredes |
| author_sort | A. Lago |
| collection | DOAJ |
| description | Abstract This study introduces groundbreaking cryopreservation, being the first to successfully cryopreserve the juvenile stage of a marine organism, the largest marine organism ever cryopreserved. By optimizing feeding strategies, equilibration times, and cryoprotectant concentrations, we extended our initial protocol for young larvae to include complex larval stages (72 h–26 days post-fertilization) and juveniles (40–45 days, up to 1 mm). These protocols mark a significant step in biodiversity conservation, seedstock preservation, genetic line maintenance, and sustainability of overexploited mollusk populations. |
| format | Article |
| id | doaj-art-2eeb7a4a44e141b6bccc19e9319eeb79 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2eeb7a4a44e141b6bccc19e9319eeb792025-08-20T03:04:38ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-11439-3Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquacultureA. Lago0J. Troncoso1E. Paredes2Centro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoCentro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoCentro de Investigación Mariña (CIM), Departamento de Ecoloxía e Bioloxía Animal, Grupo ECOCOST, Universidade de VigoAbstract This study introduces groundbreaking cryopreservation, being the first to successfully cryopreserve the juvenile stage of a marine organism, the largest marine organism ever cryopreserved. By optimizing feeding strategies, equilibration times, and cryoprotectant concentrations, we extended our initial protocol for young larvae to include complex larval stages (72 h–26 days post-fertilization) and juveniles (40–45 days, up to 1 mm). These protocols mark a significant step in biodiversity conservation, seedstock preservation, genetic line maintenance, and sustainability of overexploited mollusk populations.https://doi.org/10.1038/s41598-025-11439-3 |
| spellingShingle | A. Lago J. Troncoso E. Paredes Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture Scientific Reports |
| title | Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| title_full | Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| title_fullStr | Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| title_full_unstemmed | Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| title_short | Cryopreservation of juvenile Mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| title_sort | cryopreservation of juvenile mytilus galloprovincialis to safeguard mollusk biodiversity and support aquaculture |
| url | https://doi.org/10.1038/s41598-025-11439-3 |
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