Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i>
The optimization of bioactive compound extraction from <i>Fucus vesiculosus</i> using ultrasound-assisted extraction (UAE) via sonotrode was investigated to maximize phenolic recovery and antioxidant activity while promoting a sustainable process. Optimal conditions (40% <i>v</i...
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2025-01-01
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| Series: | Marine Drugs |
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| author | Lidia Gil-Martínez Alejandro Santos-Mejías José Manuel De la Torre-Ramírez Alberto Baños Vito Verardo Ana M. Gómez-Caravaca |
| author_facet | Lidia Gil-Martínez Alejandro Santos-Mejías José Manuel De la Torre-Ramírez Alberto Baños Vito Verardo Ana M. Gómez-Caravaca |
| author_sort | Lidia Gil-Martínez |
| collection | DOAJ |
| description | The optimization of bioactive compound extraction from <i>Fucus vesiculosus</i> using ultrasound-assisted extraction (UAE) via sonotrode was investigated to maximize phenolic recovery and antioxidant activity while promoting a sustainable process. Optimal conditions (40% <i>v</i>/<i>v</i> ethanol in water, 38 min, 36% amplitude) were selected to maximize phenolic recovery while considering environmental and energy sustainability by optimizing extraction efficiency and minimizing solvent and energy usage. HPLC-ESI-QTOF-MS analysis tentatively identified 25 phenolic compounds, including sulfated phenolic acids, phlorotannins, flavonoids, and halophenols, with some reported for the first time in <i>F. vesiculosus</i>, underscoring the complexity of this alga’s metabolome. The antioxidant activity of the optimized extract was evaluated through FRAP (143.7 µmol TE/g), DPPH (EC<sub>50</sub> 105.6 µg/mL), and TEAC (189.1 µmol Trolox/g) assays. The optimized process highlights <i>F. vesiculosus</i> as a valuable source of natural antioxidants, with potential applications in biotechnology, cosmetics, and food industries. |
| format | Article |
| id | doaj-art-2ee8919a0d094caaa3cf69acc8656737 |
| institution | Kabale University |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-2ee8919a0d094caaa3cf69acc86567372025-01-24T13:39:35ZengMDPI AGMarine Drugs1660-33972025-01-012314010.3390/md23010040Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i>Lidia Gil-Martínez0Alejandro Santos-Mejías1José Manuel De la Torre-Ramírez2Alberto Baños3Vito Verardo4Ana M. Gómez-Caravaca5Department of Chemistry and Natural Products, DMC Research Center, Camino de Jayena s/n, 18620 Alhendín, SpainEpiChron Research Group, Aragon Health Sciences Institute (IACS), IIS Aragón, Miguel Servet University Hospital, 50009 Zaragoza, SpainDepartment of Chemistry and Natural Products, DMC Research Center, Camino de Jayena s/n, 18620 Alhendín, SpainDepartment of Microbiology, DMC Research Center, Camino de Jayena s/n, 18620 Alhendín, SpainDepartment of Nutrition and Food Science, University of Granada, Campus of Cartuja s/n, 18071 Granada, SpainInstitute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research Center, University of Granada, 18071 Granada, SpainThe optimization of bioactive compound extraction from <i>Fucus vesiculosus</i> using ultrasound-assisted extraction (UAE) via sonotrode was investigated to maximize phenolic recovery and antioxidant activity while promoting a sustainable process. Optimal conditions (40% <i>v</i>/<i>v</i> ethanol in water, 38 min, 36% amplitude) were selected to maximize phenolic recovery while considering environmental and energy sustainability by optimizing extraction efficiency and minimizing solvent and energy usage. HPLC-ESI-QTOF-MS analysis tentatively identified 25 phenolic compounds, including sulfated phenolic acids, phlorotannins, flavonoids, and halophenols, with some reported for the first time in <i>F. vesiculosus</i>, underscoring the complexity of this alga’s metabolome. The antioxidant activity of the optimized extract was evaluated through FRAP (143.7 µmol TE/g), DPPH (EC<sub>50</sub> 105.6 µg/mL), and TEAC (189.1 µmol Trolox/g) assays. The optimized process highlights <i>F. vesiculosus</i> as a valuable source of natural antioxidants, with potential applications in biotechnology, cosmetics, and food industries.https://www.mdpi.com/1660-3397/23/1/40brown seaweedsBox–Behnken designchlorophenolsbromophenolsantioxidant capacitysulfated phenols |
| spellingShingle | Lidia Gil-Martínez Alejandro Santos-Mejías José Manuel De la Torre-Ramírez Alberto Baños Vito Verardo Ana M. Gómez-Caravaca Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> Marine Drugs brown seaweeds Box–Behnken design chlorophenols bromophenols antioxidant capacity sulfated phenols |
| title | Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> |
| title_full | Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> |
| title_fullStr | Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> |
| title_full_unstemmed | Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> |
| title_short | Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of <i>Fucus vesiculosus</i> |
| title_sort | optimization of a sonotrode extraction method and new insight of phenolic composition of i fucus vesiculosus i |
| topic | brown seaweeds Box–Behnken design chlorophenols bromophenols antioxidant capacity sulfated phenols |
| url | https://www.mdpi.com/1660-3397/23/1/40 |
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