Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i>
<b>Background/Objectives:</b> Plantaricins without a signal sequence were synthesized based on bacteriocins, plantaricins A, E, F, J, and K, of <i>Lactiplantibacillus plantarum</i> KM2. The antibacterial activities of four combinations of synthetic plantaricins—spPlnA, E&...
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MDPI AG
2025-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/3/311 |
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| author | Seung-Eun Oh Sojeong Heo Gawon Lee Jina Kim Mi-Sun Kwak Do-Won Jeong |
| author_facet | Seung-Eun Oh Sojeong Heo Gawon Lee Jina Kim Mi-Sun Kwak Do-Won Jeong |
| author_sort | Seung-Eun Oh |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Plantaricins without a signal sequence were synthesized based on bacteriocins, plantaricins A, E, F, J, and K, of <i>Lactiplantibacillus plantarum</i> KM2. The antibacterial activities of four combinations of synthetic plantaricins—spPlnA, E&F, E&J, and J&K—were identified against <i>Staphylococcus aureus</i> ATCC 12692. And in this experiment, we aimed to identify the antimicrobial mechanism of the synthesized plantaricin sample against <i>S. aureus</i>. <b>Methods/Results:</b> The minimal inhibitory concentrations for each combination were 1.4 μg/mL, 1.8 μg/mL, 1.6 μg/mL, and 1.6 μg/mL, respectively. Raman spectra changed after treating <i>S. aureus</i> ATCC 12692 with synthetic plantaricins. Furthermore, transmission electron microscopy results revealed that the four synthetic plantaricin combinations could induce the cell lysis of <i>S. aureus</i> ATCC 12692. Finally, the four synthetic plantaricin combinations maintained their antibacterial effect at temperatures below 40 °C, and at pH levels of pH = (4–7). Except for spPlnJ&K, they are stable against the action of α–amylase and lysozyme. Overall, these results indicate that, excepting spPlnJ&K, the three synthetic plantaricin combinations exhibit similar antibacterial activity. <b>Conclusions:</b> Through this study, we confirmed that synthetic plantaricin exhibited antimicrobial activity against <i>S. aureus</i>, demonstrating its potential as a direct antimicrobial agent. However, since the antimicrobial activity decreased due to protease, it was confirmed that its use is limited in environments where protease is present. |
| format | Article |
| id | doaj-art-2edf1fd823014da18b0e89128b619f65 |
| institution | DOAJ |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-2edf1fd823014da18b0e89128b619f652025-08-20T02:42:38ZengMDPI AGAntibiotics2079-63822025-03-0114331110.3390/antibiotics14030311Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i>Seung-Eun Oh0Sojeong Heo1Gawon Lee2Jina Kim3Mi-Sun Kwak4Do-Won Jeong5Department of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Republic of KoreaDepartment of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Republic of KoreaDepartment of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Republic of KoreaInsight View Tech, Hwasung 18469, Republic of KoreaKookmin Bio Corporation, Seoul 02826, Republic of KoreaDepartment of Food and Nutrition, Dongduk Women’s University, Seoul 02748, Republic of Korea<b>Background/Objectives:</b> Plantaricins without a signal sequence were synthesized based on bacteriocins, plantaricins A, E, F, J, and K, of <i>Lactiplantibacillus plantarum</i> KM2. The antibacterial activities of four combinations of synthetic plantaricins—spPlnA, E&F, E&J, and J&K—were identified against <i>Staphylococcus aureus</i> ATCC 12692. And in this experiment, we aimed to identify the antimicrobial mechanism of the synthesized plantaricin sample against <i>S. aureus</i>. <b>Methods/Results:</b> The minimal inhibitory concentrations for each combination were 1.4 μg/mL, 1.8 μg/mL, 1.6 μg/mL, and 1.6 μg/mL, respectively. Raman spectra changed after treating <i>S. aureus</i> ATCC 12692 with synthetic plantaricins. Furthermore, transmission electron microscopy results revealed that the four synthetic plantaricin combinations could induce the cell lysis of <i>S. aureus</i> ATCC 12692. Finally, the four synthetic plantaricin combinations maintained their antibacterial effect at temperatures below 40 °C, and at pH levels of pH = (4–7). Except for spPlnJ&K, they are stable against the action of α–amylase and lysozyme. Overall, these results indicate that, excepting spPlnJ&K, the three synthetic plantaricin combinations exhibit similar antibacterial activity. <b>Conclusions:</b> Through this study, we confirmed that synthetic plantaricin exhibited antimicrobial activity against <i>S. aureus</i>, demonstrating its potential as a direct antimicrobial agent. However, since the antimicrobial activity decreased due to protease, it was confirmed that its use is limited in environments where protease is present.https://www.mdpi.com/2079-6382/14/3/311plantaricin<i>Staphylococcus aureus</i> ATCC 12692antibacterial activitygrowth inhibitioncell lysis |
| spellingShingle | Seung-Eun Oh Sojeong Heo Gawon Lee Jina Kim Mi-Sun Kwak Do-Won Jeong Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> Antibiotics plantaricin <i>Staphylococcus aureus</i> ATCC 12692 antibacterial activity growth inhibition cell lysis |
| title | Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> |
| title_full | Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> |
| title_fullStr | Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> |
| title_short | Antibacterial Effects of Synthetic Plantaricins Against <i>Staphylococcus aureus</i> |
| title_sort | antibacterial effects of synthetic plantaricins against i staphylococcus aureus i |
| topic | plantaricin <i>Staphylococcus aureus</i> ATCC 12692 antibacterial activity growth inhibition cell lysis |
| url | https://www.mdpi.com/2079-6382/14/3/311 |
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