Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes
The aim of this study was to detect the expression of GSK3B gene in different tissues of goats and the expression level of intramuscular precursor adipocytes at different differentiation periods, and to further reveal the effects of overexpression and interferences of GSK3B gene on lipid deposition...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Applied Animal Research |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/09712119.2025.2462577 |
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| author | Chengsi Gong Dingshuang Chen Xiaofei He Youli Wang Xinzhu Huang Yong Wang Yan Xiong Yanyan Li Jiani Xing Ruiwen Li |
| author_facet | Chengsi Gong Dingshuang Chen Xiaofei He Youli Wang Xinzhu Huang Yong Wang Yan Xiong Yanyan Li Jiani Xing Ruiwen Li |
| author_sort | Chengsi Gong |
| collection | DOAJ |
| description | The aim of this study was to detect the expression of GSK3B gene in different tissues of goats and the expression level of intramuscular precursor adipocytes at different differentiation periods, and to further reveal the effects of overexpression and interferences of GSK3B gene on lipid deposition in intramuscular precursor adipocytes of goats. The results showed that a nucleotide sequence of 1,433 bp, CDS region 1,263 bp, encoding 420 amino acids, was obtained for the goat GSK3B gene, Goat GSK3B gene expression was highest in the lungs, lowest in the longest dorsal muscle, and highest at 120 h of induced differentiation. Overexpression of GSK3B gene significantly reduced lipid droplets in intramuscular precursor adipocytes, and also significantly down-regulated the relative expression of SREBP1, CEBPβ and PPARγ (p < 0.05), with no significant changes in the expression of FASN, CEBPα, SCD and LPL (p > 0.05), GSK3B inhibition increased lipid droplets accumulation by significantly down-regulated SREBP1, CEBPβ and PPARγ expression (p < 0.05). In conclusion, GSK3B expression significantly inhibited lipid deposition in goat intramuscular precursor adipocytes. These results provide important data to further elucidate the molecular mechanisms regulating intramuscular fat formation in goats. |
| format | Article |
| id | doaj-art-2edeb97ef03b427db08f997e9a77187b |
| institution | DOAJ |
| issn | 0971-2119 0974-1844 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Applied Animal Research |
| spelling | doaj-art-2edeb97ef03b427db08f997e9a77187b2025-08-20T03:05:24ZengTaylor & Francis GroupJournal of Applied Animal Research0971-21190974-18442025-12-0153110.1080/09712119.2025.2462577Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytesChengsi Gong0Dingshuang Chen1Xiaofei He2Youli Wang3Xinzhu Huang4Yong Wang5Yan Xiong6Yanyan Li7Jiani Xing8Ruiwen Li9Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Education Ministry, Southwest Minzu University, Chengdu, People’s Republic of ChinaChengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, People’s Republic of ChinaThe aim of this study was to detect the expression of GSK3B gene in different tissues of goats and the expression level of intramuscular precursor adipocytes at different differentiation periods, and to further reveal the effects of overexpression and interferences of GSK3B gene on lipid deposition in intramuscular precursor adipocytes of goats. The results showed that a nucleotide sequence of 1,433 bp, CDS region 1,263 bp, encoding 420 amino acids, was obtained for the goat GSK3B gene, Goat GSK3B gene expression was highest in the lungs, lowest in the longest dorsal muscle, and highest at 120 h of induced differentiation. Overexpression of GSK3B gene significantly reduced lipid droplets in intramuscular precursor adipocytes, and also significantly down-regulated the relative expression of SREBP1, CEBPβ and PPARγ (p < 0.05), with no significant changes in the expression of FASN, CEBPα, SCD and LPL (p > 0.05), GSK3B inhibition increased lipid droplets accumulation by significantly down-regulated SREBP1, CEBPβ and PPARγ expression (p < 0.05). In conclusion, GSK3B expression significantly inhibited lipid deposition in goat intramuscular precursor adipocytes. These results provide important data to further elucidate the molecular mechanisms regulating intramuscular fat formation in goats.https://www.tandfonline.com/doi/10.1080/09712119.2025.2462577GoatGSK3Bgene interferenceoverexpressionintramuscular precursor adipocytes |
| spellingShingle | Chengsi Gong Dingshuang Chen Xiaofei He Youli Wang Xinzhu Huang Yong Wang Yan Xiong Yanyan Li Jiani Xing Ruiwen Li Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes Journal of Applied Animal Research Goat GSK3B gene interference overexpression intramuscular precursor adipocytes |
| title | Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes |
| title_full | Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes |
| title_fullStr | Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes |
| title_full_unstemmed | Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes |
| title_short | Cloning and sequence analysis of goat GSK3B gene and its effect on lipogenesis in goat intramuscular adipocytes |
| title_sort | cloning and sequence analysis of goat gsk3b gene and its effect on lipogenesis in goat intramuscular adipocytes |
| topic | Goat GSK3B gene interference overexpression intramuscular precursor adipocytes |
| url | https://www.tandfonline.com/doi/10.1080/09712119.2025.2462577 |
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