Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment.
<h4>Purpose</h4>To measure vitreous levels of Lipocalin2 (LCN2) in patients with rhegmatogenous retinal detachment (RRD) and investigate potential association with presence of proliferative vitreoretinopathy (PVR).<h4>Materials and methods</h4>Collection of undiluted vitreous...
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Public Library of Science (PLoS)
2019-01-01
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| author | Georgios Batsos Eleni Christodoulou Georgios Vartholomatos Petros Galanis Maria Stefaniotou |
| author_facet | Georgios Batsos Eleni Christodoulou Georgios Vartholomatos Petros Galanis Maria Stefaniotou |
| author_sort | Georgios Batsos |
| collection | DOAJ |
| description | <h4>Purpose</h4>To measure vitreous levels of Lipocalin2 (LCN2) in patients with rhegmatogenous retinal detachment (RRD) and investigate potential association with presence of proliferative vitreoretinopathy (PVR).<h4>Materials and methods</h4>Collection of undiluted vitreous samples from 24 patients suffering from RRD and 10 control patients undergoing vitrectomy for: vitreomacular traction (VMT) (n = 2), idiopathic epiretinal membrane (iERM) (n = 6) and full thickness macular hole (FTMH) (n = 2). Quantitative analysis of LCN2 has been made with flow cytometry. Lens status, duration of symptoms, quadrants of detachment, as well as level of PVR, were assessed. Statistical analysis included Mann-Whitney test, Kruskal-Wallis test, t-test, Spearman's correlation coefficient and Fisher's exact test.<h4>Results</h4>Median LCN2 was significantly higher in the RRD group as compared to control (p<0.001). Within the RRD group there was a positive correlation between LCN2 and PVR grade (rs = 0.94, p<0.001). Median LCN2 was 35,759 pg/ml (IR = 55,347) in grade C PVR, 9,387 pg/ml (IR = 3721) in grade B, 4,917 pg/ml (IR = non computable) in grade A and 3,921 pg/ml (2132) in the no PVR group. Median LCN2 was also significantly higher in pseudophakic patients as compared to phakic patients (p = 0.007). LCN2 also correlates with the extend of detachment (≤2 vs >2 quadrants, p<0.001) as well as with duration of symptoms (rs = 0.87, p<0.001). After multivariate linear regression analysis, only PVR was independently related with LCN2 concentration. In particular, increased PVR grading was associated with increased LCN2 concentration (coefficient b = 2.97, 95% confidence interval = 1.89 to 4.67, p<0.001).<h4>Conclusion</h4>A positive correlation between vitreous levels of LCN2 and PVR grading reveals a potential role in the pathogenesis and progression of PVR. Further studies could elucidate if LCN2 could be a therapeutic target. |
| format | Article |
| id | doaj-art-2ed8df05a65f4c6ba19f4474f5a3edbd |
| institution | DOAJ |
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| language | English |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-2ed8df05a65f4c6ba19f4474f5a3edbd2025-08-20T02:55:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011412e022726610.1371/journal.pone.0227266Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment.Georgios BatsosEleni ChristodoulouGeorgios VartholomatosPetros GalanisMaria Stefaniotou<h4>Purpose</h4>To measure vitreous levels of Lipocalin2 (LCN2) in patients with rhegmatogenous retinal detachment (RRD) and investigate potential association with presence of proliferative vitreoretinopathy (PVR).<h4>Materials and methods</h4>Collection of undiluted vitreous samples from 24 patients suffering from RRD and 10 control patients undergoing vitrectomy for: vitreomacular traction (VMT) (n = 2), idiopathic epiretinal membrane (iERM) (n = 6) and full thickness macular hole (FTMH) (n = 2). Quantitative analysis of LCN2 has been made with flow cytometry. Lens status, duration of symptoms, quadrants of detachment, as well as level of PVR, were assessed. Statistical analysis included Mann-Whitney test, Kruskal-Wallis test, t-test, Spearman's correlation coefficient and Fisher's exact test.<h4>Results</h4>Median LCN2 was significantly higher in the RRD group as compared to control (p<0.001). Within the RRD group there was a positive correlation between LCN2 and PVR grade (rs = 0.94, p<0.001). Median LCN2 was 35,759 pg/ml (IR = 55,347) in grade C PVR, 9,387 pg/ml (IR = 3721) in grade B, 4,917 pg/ml (IR = non computable) in grade A and 3,921 pg/ml (2132) in the no PVR group. Median LCN2 was also significantly higher in pseudophakic patients as compared to phakic patients (p = 0.007). LCN2 also correlates with the extend of detachment (≤2 vs >2 quadrants, p<0.001) as well as with duration of symptoms (rs = 0.87, p<0.001). After multivariate linear regression analysis, only PVR was independently related with LCN2 concentration. In particular, increased PVR grading was associated with increased LCN2 concentration (coefficient b = 2.97, 95% confidence interval = 1.89 to 4.67, p<0.001).<h4>Conclusion</h4>A positive correlation between vitreous levels of LCN2 and PVR grading reveals a potential role in the pathogenesis and progression of PVR. Further studies could elucidate if LCN2 could be a therapeutic target.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227266&type=printable |
| spellingShingle | Georgios Batsos Eleni Christodoulou Georgios Vartholomatos Petros Galanis Maria Stefaniotou Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. PLoS ONE |
| title | Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. |
| title_full | Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. |
| title_fullStr | Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. |
| title_full_unstemmed | Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. |
| title_short | Vitreous levels of Lipocalin-2 on patients with primary rhegmatogenous retinal detachment. |
| title_sort | vitreous levels of lipocalin 2 on patients with primary rhegmatogenous retinal detachment |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0227266&type=printable |
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