Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant
The ornamental plant Spathiphyllum wallisii is becoming increasingly popular worldwide, and tissue culture is regarded as a practical option for its massive production. Following the multiplication phase and successive subcultures for an extended period, some morphological changes in shoots were obs...
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2024-12-01
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| Series: | Journal of Horticultural Research |
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| Online Access: | https://doi.org/10.2478/johr-2024-0015 |
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| author | Boudadi Imane Lachguer Khadija El Merzougui Soumaya El Boullani Rachida Lagram Khalid Serghini Mohammed Amine |
| author_facet | Boudadi Imane Lachguer Khadija El Merzougui Soumaya El Boullani Rachida Lagram Khalid Serghini Mohammed Amine |
| author_sort | Boudadi Imane |
| collection | DOAJ |
| description | The ornamental plant Spathiphyllum wallisii is becoming increasingly popular worldwide, and tissue culture is regarded as a practical option for its massive production. Following the multiplication phase and successive subcultures for an extended period, some morphological changes in shoots were observed in Spathiphyllum explants, primarily a reduction in leaf area. For the first time, we report the application of silver nitrate to enhance Spathiphyllum shoot and root quality. The Murashige and Skoog (MS) medium, with 30 g·L−1 of sucrose and 100 mg·L−1 of ascorbic acid, was supplemented with different concentrations of AgNO3. The addition of AgNO3 improved shoot development and leaf expansion by increasing their length and width. Specifically, at the 2.0–4.0 mg·L−1 range, silver nitrate successfully induced the highest leaf area (1.7 cm2), while stem elongation was promoted with the application of 3.0 mg·L−1 of AgNO3. Additionally, optimal leaf numbers of new shoots were observed at 1 mg·L−1. Furthermore, when used at an optimal concentration, AgNO3 significantly boosted rhizogenesis, increasing the number (9.2 roots), length (5.8 cm), and diameter (1 mm) of roots per explant. After the plantlets were transferred to pots, they acclimatized successfully with a 100% survival rate. This finding underscores the potential of silver nitrate to optimize the growth and rooting of Spathiphyllum shoots, paving the way for a more efficient micropropagation process. |
| format | Article |
| id | doaj-art-2ed70f9afe704403a1192bc63eafea6b |
| institution | DOAJ |
| issn | 2353-3978 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Horticultural Research |
| spelling | doaj-art-2ed70f9afe704403a1192bc63eafea6b2025-08-20T02:57:40ZengSciendoJournal of Horticultural Research2353-39782024-12-01322152210.2478/johr-2024-0015Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental PlantBoudadi Imane0Lachguer Khadija1El Merzougui Soumaya2El Boullani Rachida3Lagram Khalid4Serghini Mohammed Amine51Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco1Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco1Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco1Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco1Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, Morocco1Laboratory of Biotechnology and Valorization of Natural Resources, Faculty of Sciences, Ibn Zohr University, Agadir, MoroccoThe ornamental plant Spathiphyllum wallisii is becoming increasingly popular worldwide, and tissue culture is regarded as a practical option for its massive production. Following the multiplication phase and successive subcultures for an extended period, some morphological changes in shoots were observed in Spathiphyllum explants, primarily a reduction in leaf area. For the first time, we report the application of silver nitrate to enhance Spathiphyllum shoot and root quality. The Murashige and Skoog (MS) medium, with 30 g·L−1 of sucrose and 100 mg·L−1 of ascorbic acid, was supplemented with different concentrations of AgNO3. The addition of AgNO3 improved shoot development and leaf expansion by increasing their length and width. Specifically, at the 2.0–4.0 mg·L−1 range, silver nitrate successfully induced the highest leaf area (1.7 cm2), while stem elongation was promoted with the application of 3.0 mg·L−1 of AgNO3. Additionally, optimal leaf numbers of new shoots were observed at 1 mg·L−1. Furthermore, when used at an optimal concentration, AgNO3 significantly boosted rhizogenesis, increasing the number (9.2 roots), length (5.8 cm), and diameter (1 mm) of roots per explant. After the plantlets were transferred to pots, they acclimatized successfully with a 100% survival rate. This finding underscores the potential of silver nitrate to optimize the growth and rooting of Spathiphyllum shoots, paving the way for a more efficient micropropagation process.https://doi.org/10.2478/johr-2024-0015ethylene inhibitorleaf areamicropropagationrhizogenesisshoot elongation |
| spellingShingle | Boudadi Imane Lachguer Khadija El Merzougui Soumaya El Boullani Rachida Lagram Khalid Serghini Mohammed Amine Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant Journal of Horticultural Research ethylene inhibitor leaf area micropropagation rhizogenesis shoot elongation |
| title | Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant |
| title_full | Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant |
| title_fullStr | Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant |
| title_full_unstemmed | Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant |
| title_short | Silver Nitrate: A Catalyst for Unprecedented in vitro Development of Spathiphyllum wallisii, an Important Ornamental Plant |
| title_sort | silver nitrate a catalyst for unprecedented in vitro development of spathiphyllum wallisii an important ornamental plant |
| topic | ethylene inhibitor leaf area micropropagation rhizogenesis shoot elongation |
| url | https://doi.org/10.2478/johr-2024-0015 |
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