STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN
Anticancer antibiotic lidamycin is produced by Streptomyces globisporus C-1027 strain. The lidamycin biosynthesis cluster (LDM-cluster) is localized on its plasmid SGLP1. Aim. To identify and characterize plasmid-localized gene clusters potentially involved in lidamycin biosynthesis in streptomyc...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-06-01
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| Series: | Biotechnologia Acta |
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| Online Access: | https://biotechnology.kiev.ua/images/BTA/2025/3_2025/Polishchuk_3_2025.pdf |
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| author | L.V. Polishchuk |
| author_facet | L.V. Polishchuk |
| author_sort | L.V. Polishchuk |
| collection | DOAJ |
| description | Anticancer antibiotic lidamycin is produced by Streptomyces globisporus C-1027 strain. The lidamycin biosynthesis cluster (LDM-cluster) is localized on its plasmid SGLP1.
Aim. To identify and characterize plasmid-localized gene clusters potentially involved in lidamycin biosynthesis in streptomycetes.
Methods. Nucleotide sequences of streptomycetes from the Internet database Nucleotide Collection on the National Center for Biotechnology Information were objects of this study. Search for probable LDM clusters was performed using the Basic Local Alignment Search Tool. The LDM-cluster sequence of S. globisporus C-1027 was used as a query in BLASTN analysis.
Results. The database contains information on the primary structures of thousands of chromosomes and dozens of plasmids of streptomycetes, which are fully defined (Compete genome). BLASTN-analysis of primary structures of DNAs revealed the presence of probable LDM clusters in 6 streptomycete plasmids. Nucleotide sequences of 7 plasmids were only partially identical – they all contained sequences that were similar to the fragment 7,747 bp – 112,237 bp of SGLP1.
Conclusions. The findings have demonstrated that LDM clusters are predominantly localized on plasmids in Streptomyces species. Although the identified plasmids share substantial sequence similarity - spanning approximately 104.5 kb - with the reference SGLP1 plasmid, they are not genetically identical. |
| format | Article |
| id | doaj-art-2ed01c6ebfbf4da4840da78c3390131e |
| institution | DOAJ |
| issn | 2410-7751 2410-776X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj-art-2ed01c6ebfbf4da4840da78c3390131e2025-08-20T03:15:54ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2025-06-0118656810.15407/biotech18.03.065STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINL.V. Polishchuk0Zabolotny Institute of Microbiology and Virology of of the National Academy of Sciences of Ukraine, KyivZabolotny Institute of Microbiology and Virology of of the National Academy of Sciences of Ukraine, KyivAnticancer antibiotic lidamycin is produced by Streptomyces globisporus C-1027 strain. The lidamycin biosynthesis cluster (LDM-cluster) is localized on its plasmid SGLP1. Aim. To identify and characterize plasmid-localized gene clusters potentially involved in lidamycin biosynthesis in streptomycetes. Methods. Nucleotide sequences of streptomycetes from the Internet database Nucleotide Collection on the National Center for Biotechnology Information were objects of this study. Search for probable LDM clusters was performed using the Basic Local Alignment Search Tool. The LDM-cluster sequence of S. globisporus C-1027 was used as a query in BLASTN analysis. Results. The database contains information on the primary structures of thousands of chromosomes and dozens of plasmids of streptomycetes, which are fully defined (Compete genome). BLASTN-analysis of primary structures of DNAs revealed the presence of probable LDM clusters in 6 streptomycete plasmids. Nucleotide sequences of 7 plasmids were only partially identical – they all contained sequences that were similar to the fragment 7,747 bp – 112,237 bp of SGLP1. Conclusions. The findings have demonstrated that LDM clusters are predominantly localized on plasmids in Streptomyces species. Although the identified plasmids share substantial sequence similarity - spanning approximately 104.5 kb - with the reference SGLP1 plasmid, they are not genetically identical.https://biotechnology.kiev.ua/images/BTA/2025/3_2025/Polishchuk_3_2025.pdfstreptomycesclusterplasmidnucleotide sequencecomputerized analysislidamycin biosynthesis. |
| spellingShingle | L.V. Polishchuk STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN Biotechnologia Acta streptomyces cluster plasmid nucleotide sequence computerized analysis lidamycin biosynthesis. |
| title | STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN |
| title_full | STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN |
| title_fullStr | STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN |
| title_full_unstemmed | STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN |
| title_short | STREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICINSTREPTOMYCETE PLASMIDS CONTAINING BIOSYNTHESIS GENE CLUSTERS OF ANTITUMOR ANTIBIOTIC LIDAMICIN |
| title_sort | streptomycete plasmids containing biosynthesis gene clusters of antitumor antibiotic lidamicinstreptomycete plasmids containing biosynthesis gene clusters of antitumor antibiotic lidamicin |
| topic | streptomyces cluster plasmid nucleotide sequence computerized analysis lidamycin biosynthesis. |
| url | https://biotechnology.kiev.ua/images/BTA/2025/3_2025/Polishchuk_3_2025.pdf |
| work_keys_str_mv | AT lvpolishchuk streptomyceteplasmidscontainingbiosynthesisgeneclustersofantitumorantibioticlidamicinstreptomyceteplasmidscontainingbiosynthesisgeneclustersofantitumorantibioticlidamicin |