The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function
The global issue of insecticide resistance among pests is a major concern. Ectropis grisescens Warren (Lepidoptera: Geometridae), is a highly destructive leaf-eating pest distributed in tea plantations throughout China and Japan, and has exhibited resistance to various insecticides. Recent studies s...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325000028 |
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| author | Tian Gao Yong Zhang Wanpeng Sun Qiangkun Li Xueyu Huang Dian Zhi Huabin Zi Ruijie Ji Yanhua Long Chunmei Gong Yunqiu Yang |
| author_facet | Tian Gao Yong Zhang Wanpeng Sun Qiangkun Li Xueyu Huang Dian Zhi Huabin Zi Ruijie Ji Yanhua Long Chunmei Gong Yunqiu Yang |
| author_sort | Tian Gao |
| collection | DOAJ |
| description | The global issue of insecticide resistance among pests is a major concern. Ectropis grisescens Warren (Lepidoptera: Geometridae), is a highly destructive leaf-eating pest distributed in tea plantations throughout China and Japan, and has exhibited resistance to various insecticides. Recent studies suggest that insect symbionts play a role in influencing insecticide resistance, however, their specific involvement in E. grisescens remains unclear. Here, we initially selected appropriate antibiotic mixtures at a concentration of 300 μg ml−1. The bioassay results showed that the insecticide susceptibility of the E. grisescens population treated with antibiotic mixtures significantly increased exposed to bifenthrin. Comparative analysis revealed that the LC50 value, survival rate, P450 enzyme activity, and relative content of Wolbachia in the E. grisescens population treated with 300 μg ml−1 tetracycline were notably lower than those treated with other antibiotics (ampicillin, gentamicin, and streptomycin). Moreover, the population treated with 2.5 mg ml−1 tetracycline exhibited even greater reductions in these parameters than the 300 μg ml−1 tetracycline-treated group. Additionally, 16S rRNA sequencing results showed a significant decrease in xenobiotics metabolism by cytochrome P450 in the E. grisescens population treated with 2.5 mg ml−1 tetracycline. Transcriptome analysis showed a significant down-regulation of two cytochrome P450 genes in E. grisescens population without Wolbachia. These results suggest that Wolbachia may contribute to the resistance of E. grisescens to bifenthrin by regulating cytochrome P450 genes, providing a foundation for further study on the mechanism of symbiont-mediated host detoxification metabolism in insect pests. |
| format | Article |
| id | doaj-art-2eb9859ed27549c6849be38a4a443cb0 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-2eb9859ed27549c6849be38a4a443cb02025-01-23T05:26:02ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117666The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification functionTian Gao0Yong Zhang1Wanpeng Sun2Qiangkun Li3Xueyu Huang4Dian Zhi5Huabin Zi6Ruijie Ji7Yanhua Long8Chunmei Gong9Yunqiu Yang10State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China; College of Horticulture, Northwest A＆F University, Yangling, Shaanxi, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, ChinaSchool of Life Sciences, Anhui Agricultural University, Hefei, ChinaCollege of Horticulture, Northwest A＆F University, Yangling, Shaanxi, China; Corresponding authors.State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, China; Corresponding authors.The global issue of insecticide resistance among pests is a major concern. Ectropis grisescens Warren (Lepidoptera: Geometridae), is a highly destructive leaf-eating pest distributed in tea plantations throughout China and Japan, and has exhibited resistance to various insecticides. Recent studies suggest that insect symbionts play a role in influencing insecticide resistance, however, their specific involvement in E. grisescens remains unclear. Here, we initially selected appropriate antibiotic mixtures at a concentration of 300 μg ml−1. The bioassay results showed that the insecticide susceptibility of the E. grisescens population treated with antibiotic mixtures significantly increased exposed to bifenthrin. Comparative analysis revealed that the LC50 value, survival rate, P450 enzyme activity, and relative content of Wolbachia in the E. grisescens population treated with 300 μg ml−1 tetracycline were notably lower than those treated with other antibiotics (ampicillin, gentamicin, and streptomycin). Moreover, the population treated with 2.5 mg ml−1 tetracycline exhibited even greater reductions in these parameters than the 300 μg ml−1 tetracycline-treated group. Additionally, 16S rRNA sequencing results showed a significant decrease in xenobiotics metabolism by cytochrome P450 in the E. grisescens population treated with 2.5 mg ml−1 tetracycline. Transcriptome analysis showed a significant down-regulation of two cytochrome P450 genes in E. grisescens population without Wolbachia. These results suggest that Wolbachia may contribute to the resistance of E. grisescens to bifenthrin by regulating cytochrome P450 genes, providing a foundation for further study on the mechanism of symbiont-mediated host detoxification metabolism in insect pests.http://www.sciencedirect.com/science/article/pii/S0147651325000028Ectropis grisescensBifenthrinDetoxificationP450Wolbachia |
| spellingShingle | Tian Gao Yong Zhang Wanpeng Sun Qiangkun Li Xueyu Huang Dian Zhi Huabin Zi Ruijie Ji Yanhua Long Chunmei Gong Yunqiu Yang The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function Ecotoxicology and Environmental Safety Ectropis grisescens Bifenthrin Detoxification P450 Wolbachia |
| title | The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function |
| title_full | The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function |
| title_fullStr | The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function |
| title_full_unstemmed | The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function |
| title_short | The symbiont Wolbachia increases resistance to bifenthrin in Ectropis grisescens by regulating the host detoxification function |
| title_sort | symbiont wolbachia increases resistance to bifenthrin in ectropis grisescens by regulating the host detoxification function |
| topic | Ectropis grisescens Bifenthrin Detoxification P450 Wolbachia |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325000028 |
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