Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons
The periaqueductal gray (PAG) is involved in the central regulation of nociceptive transmission by affecting the descending inhibitory pathway. In the present study, we have addressed the functional role of presynaptic glycine receptors in spontaneous glutamatergic transmission. Spontaneous EPSCs (s...
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Wiley
2013-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2013/954302 |
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| author | Kwi-Hyung Choi Michiko Nakamura Il-Sung Jang |
| author_facet | Kwi-Hyung Choi Michiko Nakamura Il-Sung Jang |
| author_sort | Kwi-Hyung Choi |
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| description | The periaqueductal gray (PAG) is involved in the central regulation of nociceptive transmission by affecting the descending inhibitory pathway. In the present study, we have addressed the functional role of presynaptic glycine receptors in spontaneous glutamatergic transmission. Spontaneous EPSCs (sEPSCs) were recorded in mechanically dissociated rat PAG neurons using a conventional whole-cell patch recording technique under voltage-clamp conditions. The application of glycine (100 µM) significantly increased the frequency of sEPSCs, without affecting the amplitude of sEPSCs. The glycine-induced increase in sEPSC frequency was blocked by 1 µM strychnine, a specific glycine receptor antagonist. The results suggest that glycine acts on presynaptic glycine receptors to increase the probability of glutamate release from excitatory nerve terminals. The glycine-induced increase in sEPSC frequency completely disappeared either in the presence of tetrodotoxin or Cd2+, voltage-gated Na+, or Ca2+ channel blockers, suggesting that the activation of presynaptic glycine receptors might depolarize excitatory nerve terminals. The present results suggest that presynaptic glycine receptors can regulate the excitability of PAG neurons by enhancing glutamatergic transmission and therefore play an important role in the regulation of various physiological functions mediated by the PAG. |
| format | Article |
| id | doaj-art-2eb37dd2558a469aaa8ef3ae5a58dd59 |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Neural Plasticity |
| spelling | doaj-art-2eb37dd2558a469aaa8ef3ae5a58dd592025-08-20T02:38:50ZengWileyNeural Plasticity2090-59041687-54432013-01-01201310.1155/2013/954302954302Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray NeuronsKwi-Hyung Choi0Michiko Nakamura1Il-Sung Jang2Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of KoreaDepartment of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of KoreaDepartment of Pharmacology, School of Dentistry, Kyungpook National University, Daegu 700-412, Republic of KoreaThe periaqueductal gray (PAG) is involved in the central regulation of nociceptive transmission by affecting the descending inhibitory pathway. In the present study, we have addressed the functional role of presynaptic glycine receptors in spontaneous glutamatergic transmission. Spontaneous EPSCs (sEPSCs) were recorded in mechanically dissociated rat PAG neurons using a conventional whole-cell patch recording technique under voltage-clamp conditions. The application of glycine (100 µM) significantly increased the frequency of sEPSCs, without affecting the amplitude of sEPSCs. The glycine-induced increase in sEPSC frequency was blocked by 1 µM strychnine, a specific glycine receptor antagonist. The results suggest that glycine acts on presynaptic glycine receptors to increase the probability of glutamate release from excitatory nerve terminals. The glycine-induced increase in sEPSC frequency completely disappeared either in the presence of tetrodotoxin or Cd2+, voltage-gated Na+, or Ca2+ channel blockers, suggesting that the activation of presynaptic glycine receptors might depolarize excitatory nerve terminals. The present results suggest that presynaptic glycine receptors can regulate the excitability of PAG neurons by enhancing glutamatergic transmission and therefore play an important role in the regulation of various physiological functions mediated by the PAG.http://dx.doi.org/10.1155/2013/954302 |
| spellingShingle | Kwi-Hyung Choi Michiko Nakamura Il-Sung Jang Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons Neural Plasticity |
| title | Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons |
| title_full | Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons |
| title_fullStr | Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons |
| title_full_unstemmed | Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons |
| title_short | Presynaptic Glycine Receptors Increase GABAergic Neurotransmission in Rat Periaqueductal Gray Neurons |
| title_sort | presynaptic glycine receptors increase gabaergic neurotransmission in rat periaqueductal gray neurons |
| url | http://dx.doi.org/10.1155/2013/954302 |
| work_keys_str_mv | AT kwihyungchoi presynapticglycinereceptorsincreasegabaergicneurotransmissioninratperiaqueductalgrayneurons AT michikonakamura presynapticglycinereceptorsincreasegabaergicneurotransmissioninratperiaqueductalgrayneurons AT ilsungjang presynapticglycinereceptorsincreasegabaergicneurotransmissioninratperiaqueductalgrayneurons |