Study of Thioredoxin Isolated from Human Blood Serum
The research included the separation of thioredoxin (Trx) from human blood serum using different biochemical techniques. One proteinous peak had been isolated by gel filtration using sephadex )G-50) that produced by ammonium sulphate precipitation(65%) after dialysis. It was noted that there are t...
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Tikrit University
2023-02-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/1190 |
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| author | Luay A. Al-Helaly Amera A. Hamdon |
| author_facet | Luay A. Al-Helaly Amera A. Hamdon |
| author_sort | Luay A. Al-Helaly |
| collection | DOAJ |
| description |
The research included the separation of thioredoxin (Trx) from human blood serum using different biochemical techniques. One proteinous peak had been isolated by gel filtration using sephadex )G-50) that produced by ammonium sulphate precipitation(65%) after dialysis. It was noted that there are two peaks of Trx when using ion exchange-type DEAE-cellulose for the product of gel filtration column.
The approximately molecular weight of the isolated Trx using gel filtration chromatography (G-50) and electrophoresis technique type SDS-PAGE were (12305 + 190) Dalton and 12461 Dalton respectively .
The results showed that inhibited and activated effects of drugs on Trx working. The results showed that inhibited to 44.59% for paracetamol, dexathamazone, diphenhydramine-HCl, theophlline anhydrous, pseudoephedrine, ceramide and caffeine anhydrous, but metronidazole and chlorpheniramine malate have activated effect to 110.8% and 21.62 respectively.
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| format | Article |
| id | doaj-art-2eb0f48df9cf46559cb0a0f508f1bdcd |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-2eb0f48df9cf46559cb0a0f508f1bdcd2025-08-20T03:50:16ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-02-0120310.25130/tjps.v20i3.1190Study of Thioredoxin Isolated from Human Blood SerumLuay A. Al-HelalyAmera A. Hamdon The research included the separation of thioredoxin (Trx) from human blood serum using different biochemical techniques. One proteinous peak had been isolated by gel filtration using sephadex )G-50) that produced by ammonium sulphate precipitation(65%) after dialysis. It was noted that there are two peaks of Trx when using ion exchange-type DEAE-cellulose for the product of gel filtration column. The approximately molecular weight of the isolated Trx using gel filtration chromatography (G-50) and electrophoresis technique type SDS-PAGE were (12305 + 190) Dalton and 12461 Dalton respectively . The results showed that inhibited and activated effects of drugs on Trx working. The results showed that inhibited to 44.59% for paracetamol, dexathamazone, diphenhydramine-HCl, theophlline anhydrous, pseudoephedrine, ceramide and caffeine anhydrous, but metronidazole and chlorpheniramine malate have activated effect to 110.8% and 21.62 respectively. https://tjpsj.org/index.php/tjps/article/view/1190IsolationThioredoxinInhibitorsActivators |
| spellingShingle | Luay A. Al-Helaly Amera A. Hamdon Study of Thioredoxin Isolated from Human Blood Serum Tikrit Journal of Pure Science Isolation Thioredoxin Inhibitors Activators |
| title | Study of Thioredoxin Isolated from Human Blood Serum |
| title_full | Study of Thioredoxin Isolated from Human Blood Serum |
| title_fullStr | Study of Thioredoxin Isolated from Human Blood Serum |
| title_full_unstemmed | Study of Thioredoxin Isolated from Human Blood Serum |
| title_short | Study of Thioredoxin Isolated from Human Blood Serum |
| title_sort | study of thioredoxin isolated from human blood serum |
| topic | Isolation Thioredoxin Inhibitors Activators |
| url | https://tjpsj.org/index.php/tjps/article/view/1190 |
| work_keys_str_mv | AT luayaalhelaly studyofthioredoxinisolatedfromhumanbloodserum AT ameraahamdon studyofthioredoxinisolatedfromhumanbloodserum |