Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR

Comparative evaluation of proteinase K and dithiothreitol as pretreatments for extracting nucleic acids from respiratory samples for multiplex PCR

Abstract Background Lower respiratory tract infections are frequently caused by bacteria, and the rapid identification of pathogens is crucial for guiding treatment. Multiplex PCR(M-PCR) can detect multiple pathogens simultaneously, but mucus and other cells in lower respiratory tract samples may in...

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Bibliographic Details
Main Authors: Yanjun Chang, Ting Li, Yanfang Niu, Xiaodong Guan, Yarong Xie
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Microbiology
Subjects:
Multiplex PCR(M-PCR)
Proteinase K(PK)
Dithiothreitol(DTT)
Sputum
Bronchoalveolar lavage fluid(BALF)
Homogenisation
Online Access:https://doi.org/10.1186/s12866-025-03956-y
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