Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2
The late endo-/lysosomal P-type ATPase ATP13A2 (PARK9) is implicated in Parkinson’s disease (PD) and Kufor-Rakeb syndrome, early-onset atypical Parkinsonism. ATP13A2 interacts at the N-terminus with the signaling lipids phosphatidic acid (PA) and phosphatidylinositol (3,5) bisphosphate (PI(3,5)P2),...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Parkinson's Disease |
| Online Access: | http://dx.doi.org/10.1155/2016/9531917 |
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| author | Shaun Martin Sarah van Veen Tine Holemans Seyma Demirsoy Chris van den Haute Veerle Baekelandt Patrizia Agostinis Jan Eggermont Peter Vangheluwe |
| author_facet | Shaun Martin Sarah van Veen Tine Holemans Seyma Demirsoy Chris van den Haute Veerle Baekelandt Patrizia Agostinis Jan Eggermont Peter Vangheluwe |
| author_sort | Shaun Martin |
| collection | DOAJ |
| description | The late endo-/lysosomal P-type ATPase ATP13A2 (PARK9) is implicated in Parkinson’s disease (PD) and Kufor-Rakeb syndrome, early-onset atypical Parkinsonism. ATP13A2 interacts at the N-terminus with the signaling lipids phosphatidic acid (PA) and phosphatidylinositol (3,5) bisphosphate (PI(3,5)P2), which modulate ATP13A2 activity under cellular stress conditions. Here, we analyzed stable human SHSY5Y cell lines overexpressing wild-type (WT) or ATP13A2 mutants in which three N-terminal lipid binding sites (LBS1–3) were mutated. We explored the regulatory role of LBS1–3 in the cellular protection by ATP13A2 against mitochondrial stress induced by rotenone and found that the LBS2-3 mutants displayed an abrogated protective effect. Moreover, in contrast to WT, the LBS2 and LBS3 mutants responded poorly to pharmacological inhibition of, respectively, PI(3,5)P2 and PA formation. We further demonstrate that PA and PI(3,5)P2 are also required for the ATP13A2-mediated protection against the toxic metals Mn2+, Zn2+, and Fe3+, suggesting a general lipid-dependent activation mechanism of ATP13A2 in various PD-related stress conditions. Our results indicate that the ATP13A2-mediated protection requires binding of PI(3,5)P2 to LBS2 and PA to LBS3. Thus, targeting the N-terminal lipid binding sites of ATP13A2 might offer a therapeutic approach to reduce cellular toxicity of various PD insults including mitochondrial stress. |
| format | Article |
| id | doaj-art-2e98e0578f4a4c5fa1ea12d8a723fa17 |
| institution | Kabale University |
| issn | 2090-8083 2042-0080 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Parkinson's Disease |
| spelling | doaj-art-2e98e0578f4a4c5fa1ea12d8a723fa172025-02-03T01:22:44ZengWileyParkinson's Disease2090-80832042-00802016-01-01201610.1155/2016/95319179531917Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2Shaun Martin0Sarah van Veen1Tine Holemans2Seyma Demirsoy3Chris van den Haute4Veerle Baekelandt5Patrizia Agostinis6Jan Eggermont7Peter Vangheluwe8Laboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumLaboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumLaboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumLaboratory of Cell Death Research and Therapy, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumResearch Group for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, Kapucijnenvoer 33, Blok i, P.O. Box 7001, 3000 Leuven, BelgiumResearch Group for Neurobiology and Gene Therapy, Department of Neurosciences, KU Leuven, Kapucijnenvoer 33, Blok i, P.O. Box 7001, 3000 Leuven, BelgiumLaboratory of Cell Death Research and Therapy, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumLaboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumLaboratory of Cellular Transport Systems, Department of Cellular and Molecular Medicine, KU Leuven, Campus Gasthuisberg O&N 1, Herestraat 49, P.O. Box 802, 3000 Leuven, BelgiumThe late endo-/lysosomal P-type ATPase ATP13A2 (PARK9) is implicated in Parkinson’s disease (PD) and Kufor-Rakeb syndrome, early-onset atypical Parkinsonism. ATP13A2 interacts at the N-terminus with the signaling lipids phosphatidic acid (PA) and phosphatidylinositol (3,5) bisphosphate (PI(3,5)P2), which modulate ATP13A2 activity under cellular stress conditions. Here, we analyzed stable human SHSY5Y cell lines overexpressing wild-type (WT) or ATP13A2 mutants in which three N-terminal lipid binding sites (LBS1–3) were mutated. We explored the regulatory role of LBS1–3 in the cellular protection by ATP13A2 against mitochondrial stress induced by rotenone and found that the LBS2-3 mutants displayed an abrogated protective effect. Moreover, in contrast to WT, the LBS2 and LBS3 mutants responded poorly to pharmacological inhibition of, respectively, PI(3,5)P2 and PA formation. We further demonstrate that PA and PI(3,5)P2 are also required for the ATP13A2-mediated protection against the toxic metals Mn2+, Zn2+, and Fe3+, suggesting a general lipid-dependent activation mechanism of ATP13A2 in various PD-related stress conditions. Our results indicate that the ATP13A2-mediated protection requires binding of PI(3,5)P2 to LBS2 and PA to LBS3. Thus, targeting the N-terminal lipid binding sites of ATP13A2 might offer a therapeutic approach to reduce cellular toxicity of various PD insults including mitochondrial stress.http://dx.doi.org/10.1155/2016/9531917 |
| spellingShingle | Shaun Martin Sarah van Veen Tine Holemans Seyma Demirsoy Chris van den Haute Veerle Baekelandt Patrizia Agostinis Jan Eggermont Peter Vangheluwe Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 Parkinson's Disease |
| title | Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 |
| title_full | Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 |
| title_fullStr | Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 |
| title_full_unstemmed | Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 |
| title_short | Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 |
| title_sort | protection against mitochondrial and metal toxicity depends on functional lipid binding sites in atp13a2 |
| url | http://dx.doi.org/10.1155/2016/9531917 |
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