Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products
This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by <i>Yarrowia lipolytica</i> using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate (PODD) were utilized as substra...
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MDPI AG
2024-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/11/1/3 |
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| author | Camila P. L. Souza Adejanildo da S. Pereira Érika C. G. Aguieiras Priscilla F. F. Amaral |
| author_facet | Camila P. L. Souza Adejanildo da S. Pereira Érika C. G. Aguieiras Priscilla F. F. Amaral |
| author_sort | Camila P. L. Souza |
| collection | DOAJ |
| description | This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by <i>Yarrowia lipolytica</i> using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate (PODD) were utilized as substrates in both fermentation stages. Solid-state fermentation (SSF) yielded significant lipase activity when palm fiber was used alone (1.55 U/g in 48 h), while submerged fermentation (SmF) showed improved enzymatic production with the combination of fiber and PODD (1171 U/L in 72 h). The integration of SSF and SmF in SeqF achieved superior lipase activities, reaching 4464.5 U/L, an 8.3-fold increase compared to SmF alone, in Erlenmeyer flasks. SeqF-lyophilized biocatalysts from Erlenmeyer experiments showed better hydrolytic activity (131 U/g) when the best conditions were reproduced in a 4 L bioreactor (33 U/g). The SeqF-lyophilized biocatalyst was employed in esterification reactions to synthesize mono- and diacylglycerols, achieving a 24.3% conversion rate. The study highlights SeqF as a promising and sustainable approach for valorizing agro-industrial residues, contributing to biocatalyst production and advancing circular bioeconomy initiatives. |
| format | Article |
| id | doaj-art-2e8ed08000854ee586809a7fc8d6f832 |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-2e8ed08000854ee586809a7fc8d6f8322025-01-24T13:32:00ZengMDPI AGFermentation2311-56372024-12-01111310.3390/fermentation11010003Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-ProductsCamila P. L. Souza0Adejanildo da S. Pereira1Érika C. G. Aguieiras2Priscilla F. F. Amaral3By&Bio—Byproducts to Bioproducts Laboratory, Biochemical Engineering Department, School of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149–CT, Bl. E, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilBy&Bio—Byproducts to Bioproducts Laboratory, Biochemical Engineering Department, School of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149–CT, Bl. E, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilFederal University of Rio de Janeiro, Campus UFRJ Duque de Caxias Professor Geraldo Cidade, Rodovia Washington Luiz, 19.593, Duque de Caxias 25240-005, BrazilBy&Bio—Byproducts to Bioproducts Laboratory, Biochemical Engineering Department, School of Chemistry, Federal University of Rio de Janeiro, Av. Athos da Silveira Ramos, 149–CT, Bl. E, Ilha do Fundão, Rio de Janeiro 21941-909, BrazilThis study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by <i>Yarrowia lipolytica</i> using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate (PODD) were utilized as substrates in both fermentation stages. Solid-state fermentation (SSF) yielded significant lipase activity when palm fiber was used alone (1.55 U/g in 48 h), while submerged fermentation (SmF) showed improved enzymatic production with the combination of fiber and PODD (1171 U/L in 72 h). The integration of SSF and SmF in SeqF achieved superior lipase activities, reaching 4464.5 U/L, an 8.3-fold increase compared to SmF alone, in Erlenmeyer flasks. SeqF-lyophilized biocatalysts from Erlenmeyer experiments showed better hydrolytic activity (131 U/g) when the best conditions were reproduced in a 4 L bioreactor (33 U/g). The SeqF-lyophilized biocatalyst was employed in esterification reactions to synthesize mono- and diacylglycerols, achieving a 24.3% conversion rate. The study highlights SeqF as a promising and sustainable approach for valorizing agro-industrial residues, contributing to biocatalyst production and advancing circular bioeconomy initiatives.https://www.mdpi.com/2311-5637/11/1/3enzyme productionagro-industrial by-productssequential fermentation<i>Yarrowia lipolytica</i> |
| spellingShingle | Camila P. L. Souza Adejanildo da S. Pereira Érika C. G. Aguieiras Priscilla F. F. Amaral Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products Fermentation enzyme production agro-industrial by-products sequential fermentation <i>Yarrowia lipolytica</i> |
| title | Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products |
| title_full | Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products |
| title_fullStr | Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products |
| title_full_unstemmed | Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products |
| title_short | Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products |
| title_sort | sequential solid state and submerged fermentation to increase i yarrowia lipolytica i lipase production from palm oil production chain by products |
| topic | enzyme production agro-industrial by-products sequential fermentation <i>Yarrowia lipolytica</i> |
| url | https://www.mdpi.com/2311-5637/11/1/3 |
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