Sequential Solid-State and Submerged Fermentation to Increase <i>Yarrowia lipolytica</i> Lipase Production from Palm Oil Production Chain By-Products
This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by <i>Yarrowia lipolytica</i> using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate (PODD) were utilized as substra...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/11/1/3 |
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| Summary: | This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by <i>Yarrowia lipolytica</i> using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate (PODD) were utilized as substrates in both fermentation stages. Solid-state fermentation (SSF) yielded significant lipase activity when palm fiber was used alone (1.55 U/g in 48 h), while submerged fermentation (SmF) showed improved enzymatic production with the combination of fiber and PODD (1171 U/L in 72 h). The integration of SSF and SmF in SeqF achieved superior lipase activities, reaching 4464.5 U/L, an 8.3-fold increase compared to SmF alone, in Erlenmeyer flasks. SeqF-lyophilized biocatalysts from Erlenmeyer experiments showed better hydrolytic activity (131 U/g) when the best conditions were reproduced in a 4 L bioreactor (33 U/g). The SeqF-lyophilized biocatalyst was employed in esterification reactions to synthesize mono- and diacylglycerols, achieving a 24.3% conversion rate. The study highlights SeqF as a promising and sustainable approach for valorizing agro-industrial residues, contributing to biocatalyst production and advancing circular bioeconomy initiatives. |
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| ISSN: | 2311-5637 |