Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i>
The valorization of agricultural residues, particularly corn stover, represents a sustainable approach for resource utilization and protein production in which high-performing microbial strains are essential. This study systematically evaluated fungal lignocellulolytic capabilities during corn stove...
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MDPI AG
2025-04-01
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| author | Fengyun Ren Fan Wu Le Gao Yucheng Jie Xin Wu |
| author_facet | Fengyun Ren Fan Wu Le Gao Yucheng Jie Xin Wu |
| author_sort | Fengyun Ren |
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| description | The valorization of agricultural residues, particularly corn stover, represents a sustainable approach for resource utilization and protein production in which high-performing microbial strains are essential. This study systematically evaluated fungal lignocellulolytic capabilities during corn stover solid-state fermentation and employed atmospheric and room-temperature plasma (ARTP) mutagenesis to enhance the degradative capacity of <i>Trichoderma longibrachiatum</i>. Comparative screening revealed that <i>T. longibrachiatum</i> exhibited superior comprehensive degradation of the major lignocellulosic components compared to other tested strains. ARTP mutagenesis yielded mutant strain TL-MU07, which displayed significantly enhanced enzymatic capabilities with improvements in FPase (22.1%), CMCase (10.1%), and xylanase (16.1%) activities, resulting in increased cellulose degradation (14.6%) and protein accumulation (14.7%). Proteomic analysis revealed 289 significantly differentially expressed proteins, with pathway enrichment demonstrating enhancement of glycosaminoglycan degradation, amino sugar metabolism, and membrane remodeling. Key mechanistic adaptations included downregulation of Zn(2)-C6 transcriptional repressors, upregulation of detoxification enzymes (ALDH-like proteins), and enhanced secretory pathway components. The ARTP-derived mutant strain TL-MU07 represents a valuable microbial resource for agricultural waste bioconversion, offering enhanced lignocellulolytic capabilities for industrial applications while elucidating specific proteomic changes associated with improved biomass degradation efficiency for sustainable protein production in the circular bioeconomy. |
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| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-2e89c128338546abba6f986d1fca54a82025-08-20T02:28:28ZengMDPI AGFermentation2311-56372025-04-0111418110.3390/fermentation11040181Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i>Fengyun Ren0Fan Wu1Le Gao2Yucheng Jie3Xin Wu4College of Agronomy, Hunan Agricultural University, Changsha 410127, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, National Technology Innovation Center for Synthetic Biology, Tianjin 300308, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, National Technology Innovation Center for Synthetic Biology, Tianjin 300308, ChinaCollege of Agronomy, Hunan Agricultural University, Changsha 410127, ChinaTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, National Technology Innovation Center for Synthetic Biology, Tianjin 300308, ChinaThe valorization of agricultural residues, particularly corn stover, represents a sustainable approach for resource utilization and protein production in which high-performing microbial strains are essential. This study systematically evaluated fungal lignocellulolytic capabilities during corn stover solid-state fermentation and employed atmospheric and room-temperature plasma (ARTP) mutagenesis to enhance the degradative capacity of <i>Trichoderma longibrachiatum</i>. Comparative screening revealed that <i>T. longibrachiatum</i> exhibited superior comprehensive degradation of the major lignocellulosic components compared to other tested strains. ARTP mutagenesis yielded mutant strain TL-MU07, which displayed significantly enhanced enzymatic capabilities with improvements in FPase (22.1%), CMCase (10.1%), and xylanase (16.1%) activities, resulting in increased cellulose degradation (14.6%) and protein accumulation (14.7%). Proteomic analysis revealed 289 significantly differentially expressed proteins, with pathway enrichment demonstrating enhancement of glycosaminoglycan degradation, amino sugar metabolism, and membrane remodeling. Key mechanistic adaptations included downregulation of Zn(2)-C6 transcriptional repressors, upregulation of detoxification enzymes (ALDH-like proteins), and enhanced secretory pathway components. The ARTP-derived mutant strain TL-MU07 represents a valuable microbial resource for agricultural waste bioconversion, offering enhanced lignocellulolytic capabilities for industrial applications while elucidating specific proteomic changes associated with improved biomass degradation efficiency for sustainable protein production in the circular bioeconomy.https://www.mdpi.com/2311-5637/11/4/181lignocellulosic bioconversionARTP mutagenesis<i>Trichoderma longibrachiatum</i>solid-state fermentationcellulolytic enzymescomparative proteomics |
| spellingShingle | Fengyun Ren Fan Wu Le Gao Yucheng Jie Xin Wu Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> Fermentation lignocellulosic bioconversion ARTP mutagenesis <i>Trichoderma longibrachiatum</i> solid-state fermentation cellulolytic enzymes comparative proteomics |
| title | Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> |
| title_full | Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> |
| title_fullStr | Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> |
| title_full_unstemmed | Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> |
| title_short | Proteomic and Mechanistic Insights into the Efficiency of Atmospheric and Room-Temperature Plasma Mutagenesis-Driven Bioconversion of Corn Stover by <i>Trichoderma longibrachiatum</i> |
| title_sort | proteomic and mechanistic insights into the efficiency of atmospheric and room temperature plasma mutagenesis driven bioconversion of corn stover by i trichoderma longibrachiatum i |
| topic | lignocellulosic bioconversion ARTP mutagenesis <i>Trichoderma longibrachiatum</i> solid-state fermentation cellulolytic enzymes comparative proteomics |
| url | https://www.mdpi.com/2311-5637/11/4/181 |
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