Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus
Abstract Water‐soluble, stable nanoparticles of elemental sulphur with a size of 9‐52 nm have been synthesised using the stabilising potential of starch. Sulphide anions were used as sulphur precursors that were generated earlier from the bulk powder sulphur in the base‐reduction system NaOH‐N2H4·H2...
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Wiley
2021-09-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/nbt2.12044 |
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| author | Marina Lesnichaya Anastasiya Gazizova Alla Perfileva Olga Nozhkina Irina Graskova Boris Sukhov |
| author_facet | Marina Lesnichaya Anastasiya Gazizova Alla Perfileva Olga Nozhkina Irina Graskova Boris Sukhov |
| author_sort | Marina Lesnichaya |
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| description | Abstract Water‐soluble, stable nanoparticles of elemental sulphur with a size of 9‐52 nm have been synthesised using the stabilising potential of starch. Sulphide anions were used as sulphur precursors that were generated earlier from the bulk powder sulphur in the base‐reduction system NaOH‐N2H4·H2O followed by their oxidation with molecular oxygen to element sulphur atoms. Using a set of modern spectral and microscopic methods (XRD, optical spectroscopy, DLS, TEM), the phase state, elemental composition of the nanocomposites and their nanomorphological characteristics have been investigated. It was found that nanocomposites are formed as sulphur particles with the shape which is nearly spherical dispersed in the polysaccharide starch matrix with a pronounced tendency to cluster into ring formations. Water solubility and stability of the obtained nanoparticles is ensured by sorption of starch macromolecules on the surface of sulphur nanoparticles, with the thickness of the stabilising shell in a range of 10‐171 nm. In vitro experiments were carried out to study the anti‐microbial activity of the obtained sulphur nanocomposite (1.6% S) using the propidium iodide fluorescent dye staining method and the diffusion method. It showed that the water solution of the starch‐capped sulphur nanoparticles at the concentration of 6.25 µg/ml had a pronounced anti‐phytopathogenic activity against the potato ring rot pathogen Clavibacter michiganensis subsp. sepedonicus. |
| format | Article |
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| institution | Kabale University |
| issn | 1751-8741 1751-875X |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Wiley |
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| series | IET Nanobiotechnology |
| spelling | doaj-art-2e84dfa320db494f8ee86b128cdbd6a82025-08-20T03:55:07ZengWileyIET Nanobiotechnology1751-87411751-875X2021-09-0115758559310.1049/nbt2.12044Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicusMarina Lesnichaya0Anastasiya Gazizova1Alla Perfileva2Olga Nozhkina3Irina Graskova4Boris Sukhov5A. E. Favorsky Irkutsk Institute of Chemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaSiberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaSiberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaSiberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaSiberian Institute of Plant Physiology and Biochemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaA. E. Favorsky Irkutsk Institute of Chemistry Siberian Branch Russian Academy of Sciences Irkutsk RussiaAbstract Water‐soluble, stable nanoparticles of elemental sulphur with a size of 9‐52 nm have been synthesised using the stabilising potential of starch. Sulphide anions were used as sulphur precursors that were generated earlier from the bulk powder sulphur in the base‐reduction system NaOH‐N2H4·H2O followed by their oxidation with molecular oxygen to element sulphur atoms. Using a set of modern spectral and microscopic methods (XRD, optical spectroscopy, DLS, TEM), the phase state, elemental composition of the nanocomposites and their nanomorphological characteristics have been investigated. It was found that nanocomposites are formed as sulphur particles with the shape which is nearly spherical dispersed in the polysaccharide starch matrix with a pronounced tendency to cluster into ring formations. Water solubility and stability of the obtained nanoparticles is ensured by sorption of starch macromolecules on the surface of sulphur nanoparticles, with the thickness of the stabilising shell in a range of 10‐171 nm. In vitro experiments were carried out to study the anti‐microbial activity of the obtained sulphur nanocomposite (1.6% S) using the propidium iodide fluorescent dye staining method and the diffusion method. It showed that the water solution of the starch‐capped sulphur nanoparticles at the concentration of 6.25 µg/ml had a pronounced anti‐phytopathogenic activity against the potato ring rot pathogen Clavibacter michiganensis subsp. sepedonicus.https://doi.org/10.1049/nbt2.12044antibacterial activitycellular biophysicsdyesfilled polymersfluorescencemicroorganisms |
| spellingShingle | Marina Lesnichaya Anastasiya Gazizova Alla Perfileva Olga Nozhkina Irina Graskova Boris Sukhov Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus IET Nanobiotechnology antibacterial activity cellular biophysics dyes filled polymers fluorescence microorganisms |
| title | Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus |
| title_full | Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus |
| title_fullStr | Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus |
| title_full_unstemmed | Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus |
| title_short | Starch‐capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti‐phytopathogenic activity against Clavibacter sepedonicus |
| title_sort | starch capped sulphur nanoparticles synthesised from bulk powder sulphur and their anti phytopathogenic activity against clavibacter sepedonicus |
| topic | antibacterial activity cellular biophysics dyes filled polymers fluorescence microorganisms |
| url | https://doi.org/10.1049/nbt2.12044 |
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