Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan.
<h4>Background</h4>Oral squamous cell carcinoma (OSCC) is the sixth and fourth most common cause of cancer death in men worldwide and in Taiwan, respectively. AURKA, which encodes a centrosome-related serine/threonine kinase, is frequently amplified and overexpressed in many human cancer...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171583&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849765182262214656 |
|---|---|
| author | Chia-Hsuan Chou Ying-Erh Chou Chun-Yi Chuang Shun-Fa Yang Chiao-Wen Lin |
| author_facet | Chia-Hsuan Chou Ying-Erh Chou Chun-Yi Chuang Shun-Fa Yang Chiao-Wen Lin |
| author_sort | Chia-Hsuan Chou |
| collection | DOAJ |
| description | <h4>Background</h4>Oral squamous cell carcinoma (OSCC) is the sixth and fourth most common cause of cancer death in men worldwide and in Taiwan, respectively. AURKA, which encodes a centrosome-related serine/threonine kinase, is frequently amplified and overexpressed in many human cancers, particularly advanced OSCC. We conducted a hospital-based case-control study to estimate AURKA single-nucleotide polymorphisms (SNPs) and environmental risk factors to determine OSCC susceptibility and clinicopathological characteristics.<h4>Methodology/principal findings</h4>We enrolled a total of 876 OSCC patients and 1200 controls. Four SNPs of AURKA, namely rs1047972, rs2273535, rs2064863, and rs6024836, were analyzed using real-time polymerase chain reaction (PCR). Among the 1420 smokers, the AURKA polymorphism carriers with the betel nut chewing habit had a higher risk of oral cancer than AURKA wild-type (WT) carriers without the betel nut chewing habit. Patients with the AURKA rs2064863 gene had a 1.365-fold higher risk of stage III or IV OSCC (95% confidence interval [CI] 1.029-1.811) than those with the rs2064863 WT gene. Furthermore, carriers of the AURKA rs1047972/rs2273535/rs2064863 C-A-T haplotype had a 1.736-fold (95% CI 1.110-2.715) higher risk of OSCC than controls (C-T-T, the most common haplotype). Among patients with the betel quid chewing habit, carriers of other haplotypes (C-T-T, C-A-G, T-A-T, T-A-G, T-T-T, and C-T-G) had a 12.857-fold (95% CI 10.731-15.404) increased risk, and carriers of the C-A-T haplotype had the highest risk (AOR: 31.120; 95% CI 13.864-69.850) of OSCC, compared with those without the betel quid chewing who harbored other haplotypes.<h4>Conclusions</h4>In conclusion, betel nut chewing combined with the AURKA C-A-T haplotypes lead to a high risk of OSCC. These findings reveal a novel genetic-environmental predisposition for oral tumorigenesis. |
| format | Article |
| id | doaj-art-2e84585123624afa8a6fa9e7c9ffe5d2 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-2e84585123624afa8a6fa9e7c9ffe5d22025-08-20T03:04:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e017158310.1371/journal.pone.0171583Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan.Chia-Hsuan ChouYing-Erh ChouChun-Yi ChuangShun-Fa YangChiao-Wen Lin<h4>Background</h4>Oral squamous cell carcinoma (OSCC) is the sixth and fourth most common cause of cancer death in men worldwide and in Taiwan, respectively. AURKA, which encodes a centrosome-related serine/threonine kinase, is frequently amplified and overexpressed in many human cancers, particularly advanced OSCC. We conducted a hospital-based case-control study to estimate AURKA single-nucleotide polymorphisms (SNPs) and environmental risk factors to determine OSCC susceptibility and clinicopathological characteristics.<h4>Methodology/principal findings</h4>We enrolled a total of 876 OSCC patients and 1200 controls. Four SNPs of AURKA, namely rs1047972, rs2273535, rs2064863, and rs6024836, were analyzed using real-time polymerase chain reaction (PCR). Among the 1420 smokers, the AURKA polymorphism carriers with the betel nut chewing habit had a higher risk of oral cancer than AURKA wild-type (WT) carriers without the betel nut chewing habit. Patients with the AURKA rs2064863 gene had a 1.365-fold higher risk of stage III or IV OSCC (95% confidence interval [CI] 1.029-1.811) than those with the rs2064863 WT gene. Furthermore, carriers of the AURKA rs1047972/rs2273535/rs2064863 C-A-T haplotype had a 1.736-fold (95% CI 1.110-2.715) higher risk of OSCC than controls (C-T-T, the most common haplotype). Among patients with the betel quid chewing habit, carriers of other haplotypes (C-T-T, C-A-G, T-A-T, T-A-G, T-T-T, and C-T-G) had a 12.857-fold (95% CI 10.731-15.404) increased risk, and carriers of the C-A-T haplotype had the highest risk (AOR: 31.120; 95% CI 13.864-69.850) of OSCC, compared with those without the betel quid chewing who harbored other haplotypes.<h4>Conclusions</h4>In conclusion, betel nut chewing combined with the AURKA C-A-T haplotypes lead to a high risk of OSCC. These findings reveal a novel genetic-environmental predisposition for oral tumorigenesis.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171583&type=printable |
| spellingShingle | Chia-Hsuan Chou Ying-Erh Chou Chun-Yi Chuang Shun-Fa Yang Chiao-Wen Lin Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. PLoS ONE |
| title | Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. |
| title_full | Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. |
| title_fullStr | Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. |
| title_full_unstemmed | Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. |
| title_short | Combined effect of genetic polymorphisms of AURKA and environmental factors on oral cancer development in Taiwan. |
| title_sort | combined effect of genetic polymorphisms of aurka and environmental factors on oral cancer development in taiwan |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0171583&type=printable |
| work_keys_str_mv | AT chiahsuanchou combinedeffectofgeneticpolymorphismsofaurkaandenvironmentalfactorsonoralcancerdevelopmentintaiwan AT yingerhchou combinedeffectofgeneticpolymorphismsofaurkaandenvironmentalfactorsonoralcancerdevelopmentintaiwan AT chunyichuang combinedeffectofgeneticpolymorphismsofaurkaandenvironmentalfactorsonoralcancerdevelopmentintaiwan AT shunfayang combinedeffectofgeneticpolymorphismsofaurkaandenvironmentalfactorsonoralcancerdevelopmentintaiwan AT chiaowenlin combinedeffectofgeneticpolymorphismsofaurkaandenvironmentalfactorsonoralcancerdevelopmentintaiwan |