Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw.
Objective: To evaluate the quality of Lygodium japonicum (Thunb.) Sw. (L. japonicum, Hai Jin Sha) by comparing its components without stewed (W) and stewed (S) using ultra-high-performance liquid chromatography (UHPLC) and chemometric analysis. Additionally, network pharmacology was employed to inve...
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Elsevier
2025-07-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095754825000419 |
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| author | Zhiwen Duan Haibao Qiu Xiaoxia Liu Fangping Zhang Wenkai Xie Minyou He Dongmei Sun Xiangdong Chen Zhenyu Li |
| author_facet | Zhiwen Duan Haibao Qiu Xiaoxia Liu Fangping Zhang Wenkai Xie Minyou He Dongmei Sun Xiangdong Chen Zhenyu Li |
| author_sort | Zhiwen Duan |
| collection | DOAJ |
| description | Objective: To evaluate the quality of Lygodium japonicum (Thunb.) Sw. (L. japonicum, Hai Jin Sha) by comparing its components without stewed (W) and stewed (S) using ultra-high-performance liquid chromatography (UHPLC) and chemometric analysis. Additionally, network pharmacology was employed to investigate the possible mechanisms of action of L. japonicum in the urinary calculi (UC) treatment. Methods: A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry. Chemometric techniques were used to compare the L. japonicum extraction methods. Furthermore, various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC. Results: The W and S extracts were distributed into two distinct clusters. Significant differences in the levels of protocatechuic aldehyde, caffeic acid, and p-coumaric acid were observed between S and W. Network pharmacology analysis revealed that the primary targets of L. japonicum in the UC treatment were serum albumin and epidermal growth factor receptors, with potential active components including protocatechuic acid and caffeic acid. Conclusion: This study comprehensively examined the therapeutic components of L. japonicum before and after boiling, shedding light on its potential mechanisms of action in UC treatment. These findings offer valuable insights into the development and utilization of L. japonicum resources. |
| format | Article |
| id | doaj-art-2e7c6aea02fc4d78b8982eb278094dc9 |
| institution | Kabale University |
| issn | 2095-7548 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj-art-2e7c6aea02fc4d78b8982eb278094dc92025-08-20T03:31:37ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482025-07-0112343444410.1016/j.jtcms.2025.04.001Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw.Zhiwen Duan0Haibao Qiu1Xiaoxia Liu2Fangping Zhang3Wenkai Xie4Minyou He5Dongmei Sun6Xiangdong Chen7Zhenyu Li8Guangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, ChinaGuangzhou University of Chinese Medicine, Guangzhou 510006, ChinaGuangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, ChinaGuangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, ChinaGuangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, ChinaGuangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, ChinaGuangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, China; Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaGuangzhou University of Chinese Medicine, Guangzhou 510006, China; Corresponding authors.Guangdong Yifang Pharmaceutical Co. Ltd., Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula, Foshan 528244, China; Corresponding authors.Objective: To evaluate the quality of Lygodium japonicum (Thunb.) Sw. (L. japonicum, Hai Jin Sha) by comparing its components without stewed (W) and stewed (S) using ultra-high-performance liquid chromatography (UHPLC) and chemometric analysis. Additionally, network pharmacology was employed to investigate the possible mechanisms of action of L. japonicum in the urinary calculi (UC) treatment. Methods: A fingerprinting method was established to identify components through UHPLC-tandem mass spectrometry. Chemometric techniques were used to compare the L. japonicum extraction methods. Furthermore, various network pharmacological approaches were used to identify and analyze the potential targets of the identified components in relation to UC. Results: The W and S extracts were distributed into two distinct clusters. Significant differences in the levels of protocatechuic aldehyde, caffeic acid, and p-coumaric acid were observed between S and W. Network pharmacology analysis revealed that the primary targets of L. japonicum in the UC treatment were serum albumin and epidermal growth factor receptors, with potential active components including protocatechuic acid and caffeic acid. Conclusion: This study comprehensively examined the therapeutic components of L. japonicum before and after boiling, shedding light on its potential mechanisms of action in UC treatment. These findings offer valuable insights into the development and utilization of L. japonicum resources.http://www.sciencedirect.com/science/article/pii/S2095754825000419Lygodium japonicum (Thunb.) Sw.UHPLCFingerprint profilingNetwork pharmacologyCaffeic acidQuality evaluation |
| spellingShingle | Zhiwen Duan Haibao Qiu Xiaoxia Liu Fangping Zhang Wenkai Xie Minyou He Dongmei Sun Xiangdong Chen Zhenyu Li Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. Journal of Traditional Chinese Medical Sciences Lygodium japonicum (Thunb.) Sw. UHPLC Fingerprint profiling Network pharmacology Caffeic acid Quality evaluation |
| title | Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. |
| title_full | Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. |
| title_fullStr | Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. |
| title_full_unstemmed | Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. |
| title_short | Comprehensive ultra-high-performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of Lygodium japonicum (Thunb.) Sw. |
| title_sort | comprehensive ultra high performance liquid chromatography fingerprint profiling and network pharmacology analysis for the quality assessment of lygodium japonicum thunb sw |
| topic | Lygodium japonicum (Thunb.) Sw. UHPLC Fingerprint profiling Network pharmacology Caffeic acid Quality evaluation |
| url | http://www.sciencedirect.com/science/article/pii/S2095754825000419 |
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