Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis
Introduction. Tuberculosis remains a significant public health issue, ranking second only to COVID-19 in terms of mortality from infectious diseases, with more than 10 million individuals affected annually. Despite a decline in incidence rate, its diagnosis continues to be challenging, particularly...
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Bashkir State Medical University
2024-12-01
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| Series: | Креативная хирургия и онкология |
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| Online Access: | https://www.surgonco.ru/jour/article/view/1017 |
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| author | R. N. Islamov R. A. Sharipov R. K. Tukfatullin I. V. Sidorov R. K. Yagafarova |
| author_facet | R. N. Islamov R. A. Sharipov R. K. Tukfatullin I. V. Sidorov R. K. Yagafarova |
| author_sort | R. N. Islamov |
| collection | DOAJ |
| description | Introduction. Tuberculosis remains a significant public health issue, ranking second only to COVID-19 in terms of mortality from infectious diseases, with more than 10 million individuals affected annually. Despite a decline in incidence rate, its diagnosis continues to be challenging, particularly in cases with negative results from microbiological and histological examinations.Aim. To compare the effectiveness of surgical diagnostic methods for tuberculosis during the pre-analytical phase of bacteriological verification.Materials and methods. The retrospective study, conducted at the Republican Clinical Tuberculosis Treatment Dispensary of the Republic of Bashkortostan, analyzed data from 1272 patients who were hospitalized in the Tuberculous Pulmonary Surgery Unit from 2021 to 2023 and underwent bacteriological diagnostics, including microscopy and polymerase chain reaction.Results and discussion. The results indicate that out of 1272 surgeries, 65.2% involved resection procedures, with confirmed tuberculosis infection in 83.1% of patients. Bronchoalveolar lavage identified mycobacteria in 153 patients (20.5%), whereas sputum collection revealed them in 106 patients. The new collection method, although less effective, demonstrated a detection rate of 15.7% through molecular genetic analysis.Conclusion. Surgical diagnostic methods for tuberculosis appeared to be effective, detecting mycobacterium tuberculosis in 83.1% of cases, surpassing the standard sputum collection method, which has a detection rate of 10.8% via molecular genetic analysis. Bronchoalveolar lavage proved to be more productive, yielding 20.5% positive results on a molecular genetic basis, 11% microscopically, and 8% through culture. Although the new method is less effective, it is recorded as being more accurate than the standard, with detection rates of 15.7% molecularly, 6.6% microscopically, and 5.2% through culture. |
| format | Article |
| id | doaj-art-2e77deb3ed6a48d79fb850373c00238e |
| institution | DOAJ |
| issn | 2076-3093 2307-0501 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Bashkir State Medical University |
| record_format | Article |
| series | Креативная хирургия и онкология |
| spelling | doaj-art-2e77deb3ed6a48d79fb850373c00238e2025-08-20T02:53:30ZengBashkir State Medical UniversityКреативная хирургия и онкология2076-30932307-05012024-12-0114431732210.24060/2076-3093-2024-14-4-317-322597Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium TuberculosisR. N. Islamov0R. A. Sharipov1R. K. Tukfatullin2I. V. Sidorov3R. K. Yagafarova4Republican Clinical Tuberculosis Treatment DispensaryBashkir State Medical UniversityBashkir State Medical UniversityRepublican Clinical Tuberculosis Treatment DispensaryBashkir State Medical UniversityIntroduction. Tuberculosis remains a significant public health issue, ranking second only to COVID-19 in terms of mortality from infectious diseases, with more than 10 million individuals affected annually. Despite a decline in incidence rate, its diagnosis continues to be challenging, particularly in cases with negative results from microbiological and histological examinations.Aim. To compare the effectiveness of surgical diagnostic methods for tuberculosis during the pre-analytical phase of bacteriological verification.Materials and methods. The retrospective study, conducted at the Republican Clinical Tuberculosis Treatment Dispensary of the Republic of Bashkortostan, analyzed data from 1272 patients who were hospitalized in the Tuberculous Pulmonary Surgery Unit from 2021 to 2023 and underwent bacteriological diagnostics, including microscopy and polymerase chain reaction.Results and discussion. The results indicate that out of 1272 surgeries, 65.2% involved resection procedures, with confirmed tuberculosis infection in 83.1% of patients. Bronchoalveolar lavage identified mycobacteria in 153 patients (20.5%), whereas sputum collection revealed them in 106 patients. The new collection method, although less effective, demonstrated a detection rate of 15.7% through molecular genetic analysis.Conclusion. Surgical diagnostic methods for tuberculosis appeared to be effective, detecting mycobacterium tuberculosis in 83.1% of cases, surpassing the standard sputum collection method, which has a detection rate of 10.8% via molecular genetic analysis. Bronchoalveolar lavage proved to be more productive, yielding 20.5% positive results on a molecular genetic basis, 11% microscopically, and 8% through culture. Although the new method is less effective, it is recorded as being more accurate than the standard, with detection rates of 15.7% molecularly, 6.6% microscopically, and 5.2% through culture.https://www.surgonco.ru/jour/article/view/1017tuberculosismycobacterium tuberculosisbronchoscopyculture on solid mediamicroscopysputumpolymerase chain reactionbronchoalveolar lavage |
| spellingShingle | R. N. Islamov R. A. Sharipov R. K. Tukfatullin I. V. Sidorov R. K. Yagafarova Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis Креативная хирургия и онкология tuberculosis mycobacterium tuberculosis bronchoscopy culture on solid media microscopy sputum polymerase chain reaction bronchoalveolar lavage |
| title | Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis |
| title_full | Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis |
| title_fullStr | Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis |
| title_full_unstemmed | Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis |
| title_short | Effectiveness of Biological and Surgical Sampling for Detection of Mycobacterium Tuberculosis |
| title_sort | effectiveness of biological and surgical sampling for detection of mycobacterium tuberculosis |
| topic | tuberculosis mycobacterium tuberculosis bronchoscopy culture on solid media microscopy sputum polymerase chain reaction bronchoalveolar lavage |
| url | https://www.surgonco.ru/jour/article/view/1017 |
| work_keys_str_mv | AT rnislamov effectivenessofbiologicalandsurgicalsamplingfordetectionofmycobacteriumtuberculosis AT rasharipov effectivenessofbiologicalandsurgicalsamplingfordetectionofmycobacteriumtuberculosis AT rktukfatullin effectivenessofbiologicalandsurgicalsamplingfordetectionofmycobacteriumtuberculosis AT ivsidorov effectivenessofbiologicalandsurgicalsamplingfordetectionofmycobacteriumtuberculosis AT rkyagafarova effectivenessofbiologicalandsurgicalsamplingfordetectionofmycobacteriumtuberculosis |