Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells
(i) Background: The increasing prevalence of allergic diseases highlights the need for effective treatments. <i>Lonicera caerulea</i> fruit has been recognized for its anti-inflammatory, anti-cancer, and neuroprotective effects, but the mechanisms underlying its anti-allergic properties...
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2024-12-01
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| author | Ye-Eun Choi Jung-Mo Yang Chae-Won Jeong Sung-Hwan Park Hee-Won Yoo Hyun-Duck Jo Ju-Hyun Cho |
| author_facet | Ye-Eun Choi Jung-Mo Yang Chae-Won Jeong Sung-Hwan Park Hee-Won Yoo Hyun-Duck Jo Ju-Hyun Cho |
| author_sort | Ye-Eun Choi |
| collection | DOAJ |
| description | (i) Background: The increasing prevalence of allergic diseases highlights the need for effective treatments. <i>Lonicera caerulea</i> fruit has been recognized for its anti-inflammatory, anti-cancer, and neuroprotective effects, but the mechanisms underlying its anti-allergic properties remain unclear. (ii) Objective: This study aims to evaluate the total phenolic, total flavonoid, and cyanidin-3-glucoside (C3G) contents of <i>Lonicera caerulea</i> extract (HR2302-30E) and to investigate its antioxidant and anti-allergic activities. (iii) Methods: Using an IgE-stimulated RBL-2H3 cell model, we assessed the effects of HR2302-30E and C3G on mast cell degranulation, β-hexosaminidase and histamine release. Western blot analysis was performed to evaluate the expression of high-affinity IgE receptor (FcεRI)β/γ and the phosphorylation of Src family kinases (Syk, Fyn). We also examined the phosphorylation of downstream factors phospholipase Cγ, protein kinase Cδ, and mitogen-activated protein kinase. (iv) Results: Total phenolic, flavonoid, and C3G contents of HR2302-30E were 18.73 mg GAE/g, 11.83 mg QE/g, and 7.02 mg/g, respectively. In IgE-activated mast cells, HR2302-30E and C3G inhibited β-hexosaminidase and histamine release. Western blot analysis revealed reduced expression of FcεRIβ/γ and decreased phosphorylation of key downstream signaling molecules. Conclusions: These findings suggest that HR2302-30E and C3G modulate FcεRI signaling, indicating their potential as natural anti-allergic agents. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-2e76fe6608e8463594a3f96e7b3e5f9f2025-08-20T02:53:40ZengMDPI AGApplied Sciences2076-34172024-12-0114241172210.3390/app142411722Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 CellsYe-Eun Choi0Jung-Mo Yang1Chae-Won Jeong2Sung-Hwan Park3Hee-Won Yoo4Hyun-Duck Jo5Ju-Hyun Cho6Haram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of KoreaHaram Central Research Institute, Cheongju 28160, Republic of Korea(i) Background: The increasing prevalence of allergic diseases highlights the need for effective treatments. <i>Lonicera caerulea</i> fruit has been recognized for its anti-inflammatory, anti-cancer, and neuroprotective effects, but the mechanisms underlying its anti-allergic properties remain unclear. (ii) Objective: This study aims to evaluate the total phenolic, total flavonoid, and cyanidin-3-glucoside (C3G) contents of <i>Lonicera caerulea</i> extract (HR2302-30E) and to investigate its antioxidant and anti-allergic activities. (iii) Methods: Using an IgE-stimulated RBL-2H3 cell model, we assessed the effects of HR2302-30E and C3G on mast cell degranulation, β-hexosaminidase and histamine release. Western blot analysis was performed to evaluate the expression of high-affinity IgE receptor (FcεRI)β/γ and the phosphorylation of Src family kinases (Syk, Fyn). We also examined the phosphorylation of downstream factors phospholipase Cγ, protein kinase Cδ, and mitogen-activated protein kinase. (iv) Results: Total phenolic, flavonoid, and C3G contents of HR2302-30E were 18.73 mg GAE/g, 11.83 mg QE/g, and 7.02 mg/g, respectively. In IgE-activated mast cells, HR2302-30E and C3G inhibited β-hexosaminidase and histamine release. Western blot analysis revealed reduced expression of FcεRIβ/γ and decreased phosphorylation of key downstream signaling molecules. Conclusions: These findings suggest that HR2302-30E and C3G modulate FcεRI signaling, indicating their potential as natural anti-allergic agents.https://www.mdpi.com/2076-3417/14/24/11722blue honeysucklecyanidin-3-glucosidemast cell stabilizationIgE receptor signaling |
| spellingShingle | Ye-Eun Choi Jung-Mo Yang Chae-Won Jeong Sung-Hwan Park Hee-Won Yoo Hyun-Duck Jo Ju-Hyun Cho Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells Applied Sciences blue honeysuckle cyanidin-3-glucoside mast cell stabilization IgE receptor signaling |
| title | Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells |
| title_full | Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells |
| title_fullStr | Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells |
| title_full_unstemmed | Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells |
| title_short | Anti-Allergic Effects of <i>Lonicera caerulea</i> L. Extract and Cyanidin-3-Glucoside on Degranulation and FcεRI Signaling Pathway of RBL-2H3 Cells |
| title_sort | anti allergic effects of i lonicera caerulea i l extract and cyanidin 3 glucoside on degranulation and fcεri signaling pathway of rbl 2h3 cells |
| topic | blue honeysuckle cyanidin-3-glucoside mast cell stabilization IgE receptor signaling |
| url | https://www.mdpi.com/2076-3417/14/24/11722 |
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