Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates
Background and objectives: Multi-drug resistant microorganisms have caused a remarkable increase in hospital-acquired infections (HAI) during the previous few decades. Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings. Methods: A total of...
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Erbil Polytechnic University
2023-09-01
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| Series: | Polytechnic Journal |
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| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol12/iss2/9 |
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| _version_ | 1850106388070531072 |
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| author | Hawkar S. Omer Safaa T. Aka |
| author_facet | Hawkar S. Omer Safaa T. Aka |
| author_sort | Hawkar S. Omer |
| collection | DOAJ |
| description | Background and objectives: Multi-drug resistant microorganisms have caused a remarkable
increase in hospital-acquired infections (HAI) during the previous few decades. Moreover,
microbiological contamination of antiseptics and disinfectants has been recorded in medical settings.
Methods: A total of 61 clinically relevant Acinetobacter baumannii isolates were collected from
various clinical specimens in hospitals in Erbil city/Iraq between September 2021 and March 2022.
The isolates were identified using VITEK-2 compact system. Antimicrobial susceptibility testing was
carried out for a total of 16 different therapeutically relevant antibiotics in accordance with the criteria
of the Clinical Laboratory Standards Institute (CLSI) using the above mentioned system. A
spectrophotometer was used to determine the optical density at 600 nm (OD600nm) for biofilm
evaluation. Wilcoxon signed ranks test used to determine the effect of sub-MIC of CHX on the CHXMIC and biofilm formation.
Results: the study showed that there is a significant difference in CHX-MIC and biofilm formation
before and after 7 days of incubation of Acinetobacter baumannii in sub-MIC of CHX (p<0.05).
Exposure of Acinetobacter baumannii to sub-lethal CHX concentrations develops inducible resistance
to lethal CHX doses. The biofilm formation ability of isolates also increased after using sub-lethal dose
of CHX. |
| format | Article |
| id | doaj-art-2e7400d729d042cebf714d5adfcaceea |
| institution | OA Journals |
| issn | 2707-7799 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Erbil Polytechnic University |
| record_format | Article |
| series | Polytechnic Journal |
| spelling | doaj-art-2e7400d729d042cebf714d5adfcaceea2025-08-20T02:38:51ZengErbil Polytechnic UniversityPolytechnic Journal2707-77992023-09-011228591https://doi.org/10.25156/ptj.v12n2y2022.pp85-91Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii IsolatesHawkar S. Omer0Safaa T. Aka1Department of Pharmacognosy, College of Pharmacy, Hawler Medical University Department of Pharmacognosy, College of Pharmacy, Hawler Medical University Background and objectives: Multi-drug resistant microorganisms have caused a remarkable increase in hospital-acquired infections (HAI) during the previous few decades. Moreover, microbiological contamination of antiseptics and disinfectants has been recorded in medical settings. Methods: A total of 61 clinically relevant Acinetobacter baumannii isolates were collected from various clinical specimens in hospitals in Erbil city/Iraq between September 2021 and March 2022. The isolates were identified using VITEK-2 compact system. Antimicrobial susceptibility testing was carried out for a total of 16 different therapeutically relevant antibiotics in accordance with the criteria of the Clinical Laboratory Standards Institute (CLSI) using the above mentioned system. A spectrophotometer was used to determine the optical density at 600 nm (OD600nm) for biofilm evaluation. Wilcoxon signed ranks test used to determine the effect of sub-MIC of CHX on the CHXMIC and biofilm formation. Results: the study showed that there is a significant difference in CHX-MIC and biofilm formation before and after 7 days of incubation of Acinetobacter baumannii in sub-MIC of CHX (p<0.05). Exposure of Acinetobacter baumannii to sub-lethal CHX concentrations develops inducible resistance to lethal CHX doses. The biofilm formation ability of isolates also increased after using sub-lethal dose of CHX.https://polytechnic-journal.epu.edu.iq/home/vol12/iss2/9acinetobacter baumannii,chlorhexidine,biofilm,multiresistant. |
| spellingShingle | Hawkar S. Omer Safaa T. Aka Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates Polytechnic Journal acinetobacter baumannii, chlorhexidine, biofilm, multiresistant. |
| title | Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates |
| title_full | Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates |
| title_fullStr | Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates |
| title_full_unstemmed | Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates |
| title_short | Effects of Subminimal Inhibitory Concentrations of Chlorhexidine on the Chlorhexidine Resistance and Biofilm Formation in Clinical Drug-resistant Acinetobacter baumannii Isolates |
| title_sort | effects of subminimal inhibitory concentrations of chlorhexidine on the chlorhexidine resistance and biofilm formation in clinical drug resistant acinetobacter baumannii isolates |
| topic | acinetobacter baumannii, chlorhexidine, biofilm, multiresistant. |
| url | https://polytechnic-journal.epu.edu.iq/home/vol12/iss2/9 |
| work_keys_str_mv | AT hawkarsomer effectsofsubminimalinhibitoryconcentrationsofchlorhexidineonthechlorhexidineresistanceandbiofilmformationinclinicaldrugresistantacinetobacterbaumanniiisolates AT safaataka effectsofsubminimalinhibitoryconcentrationsofchlorhexidineonthechlorhexidineresistanceandbiofilmformationinclinicaldrugresistantacinetobacterbaumanniiisolates |