In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines
Abstract A series of eight metalacyclic derivatives of titanium i.e. [(acac)Ti(ph)L] (TiC1–TiC7) and [LTiL] (TiC8) (where acacH = acetylacetone, ph = phenol derivatives and L = ligand L1) were synthesized in anhydrous media and were characterized employing NMR (1H and13C), UV-Vis, FT-IR and ESI-mass...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-13995-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849766939475312640 |
|---|---|
| author | Shivabasayya V. Salimath Kavita B. Hiremath Sathish Thanigachalam Arjita Ghosh Selva Kumar Ramasamy Anbalagan Moorthy S. K. Ashok Kumar Murugesh Shivashankar Madhvesh Pathak |
| author_facet | Shivabasayya V. Salimath Kavita B. Hiremath Sathish Thanigachalam Arjita Ghosh Selva Kumar Ramasamy Anbalagan Moorthy S. K. Ashok Kumar Murugesh Shivashankar Madhvesh Pathak |
| author_sort | Shivabasayya V. Salimath |
| collection | DOAJ |
| description | Abstract A series of eight metalacyclic derivatives of titanium i.e. [(acac)Ti(ph)L] (TiC1–TiC7) and [LTiL] (TiC8) (where acacH = acetylacetone, ph = phenol derivatives and L = ligand L1) were synthesized in anhydrous media and were characterized employing NMR (1H and13C), UV-Vis, FT-IR and ESI-mass analyses. Viscosity assays, absorption and fluorescence spectroscopy and molecular docking studies have been extrapolated to investigate the complexes’ interaction with CT-DNA and bovine serum albumin (BSA). Interaction of the complexes with CT-DNA was validated as groove binding mode using UV-Vis absorption spectra as evidenced by their intrinsic binding constants of all wherein TiC1 and TiC2 exhibited higher values (19.4 × 105 and 5.50 × 105 M− 1) of Kb. Gel electrophoresis assay displayed that CT-DNA had a limited cleavage ability when metal complexes with different activators were present. Fluorescence technique also verified the interaction of the complexes with CT-DNA and BSA to demonstrate greater Kb values (2.84 × 104 and 6.42 × 104 M− 1) of the derivatives TiC1 and TiC2. DPPH assay was carried out to determine the radical scavenging activity of the complexes, so in-vitro cytotoxic effect on malignant cell lines was investigated by MTT Assay with MCF7 and HeLa cell lines, where complexes were observed as potent towards the HeLa cell line only. The potent TiC2 and TiC8 were screened with MTT assay against a normal HEK-293 cell line and were found to be inactive as expected. Subsequently, dual staining with acridine orange (AO) and ethidium bromide (EB) was performed to determine the cell death. Eventually, intracellular ROS was determined by DCFDA staining followed by cell cycle analysis was carried out using propidium iodide (PI) by flow cytometry to determine the phases. |
| format | Article |
| id | doaj-art-2e73ec553f2341aeafad60324b566c15 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2e73ec553f2341aeafad60324b566c152025-08-20T03:04:25ZengNature PortfolioScientific Reports2045-23222025-08-0115112910.1038/s41598-025-13995-0In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell linesShivabasayya V. Salimath0Kavita B. Hiremath1Sathish Thanigachalam2Arjita Ghosh3Selva Kumar Ramasamy4Anbalagan Moorthy5S. K. Ashok Kumar6Murugesh Shivashankar7Madhvesh Pathak8Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Department of Integrative Biology, School of Bioscience and Technology (SBST), Vellore Institute of Technology (VIT)Department of Chemistry, M.M. Engineering College, Maharishi Markandeshwar (Deemed to be University)Department of Integrative Biology, School of Bioscience and Technology (SBST), Vellore Institute of Technology (VIT)Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Department of Chemistry, School of Advanced Sciences (SAS), Vellore Institute of Technology (VIT)Abstract A series of eight metalacyclic derivatives of titanium i.e. [(acac)Ti(ph)L] (TiC1–TiC7) and [LTiL] (TiC8) (where acacH = acetylacetone, ph = phenol derivatives and L = ligand L1) were synthesized in anhydrous media and were characterized employing NMR (1H and13C), UV-Vis, FT-IR and ESI-mass analyses. Viscosity assays, absorption and fluorescence spectroscopy and molecular docking studies have been extrapolated to investigate the complexes’ interaction with CT-DNA and bovine serum albumin (BSA). Interaction of the complexes with CT-DNA was validated as groove binding mode using UV-Vis absorption spectra as evidenced by their intrinsic binding constants of all wherein TiC1 and TiC2 exhibited higher values (19.4 × 105 and 5.50 × 105 M− 1) of Kb. Gel electrophoresis assay displayed that CT-DNA had a limited cleavage ability when metal complexes with different activators were present. Fluorescence technique also verified the interaction of the complexes with CT-DNA and BSA to demonstrate greater Kb values (2.84 × 104 and 6.42 × 104 M− 1) of the derivatives TiC1 and TiC2. DPPH assay was carried out to determine the radical scavenging activity of the complexes, so in-vitro cytotoxic effect on malignant cell lines was investigated by MTT Assay with MCF7 and HeLa cell lines, where complexes were observed as potent towards the HeLa cell line only. The potent TiC2 and TiC8 were screened with MTT assay against a normal HEK-293 cell line and were found to be inactive as expected. Subsequently, dual staining with acridine orange (AO) and ethidium bromide (EB) was performed to determine the cell death. Eventually, intracellular ROS was determined by DCFDA staining followed by cell cycle analysis was carried out using propidium iodide (PI) by flow cytometry to determine the phases.https://doi.org/10.1038/s41598-025-13995-0 |
| spellingShingle | Shivabasayya V. Salimath Kavita B. Hiremath Sathish Thanigachalam Arjita Ghosh Selva Kumar Ramasamy Anbalagan Moorthy S. K. Ashok Kumar Murugesh Shivashankar Madhvesh Pathak In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines Scientific Reports |
| title | In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines |
| title_full | In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines |
| title_fullStr | In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines |
| title_full_unstemmed | In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines |
| title_short | In-vitro antiproliferative evaluation of newly synthesized titanium(IV) metallacyclic complexes on HeLa and MCF7 cell lines |
| title_sort | in vitro antiproliferative evaluation of newly synthesized titanium iv metallacyclic complexes on hela and mcf7 cell lines |
| url | https://doi.org/10.1038/s41598-025-13995-0 |
| work_keys_str_mv | AT shivabasayyavsalimath invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT kavitabhiremath invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT sathishthanigachalam invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT arjitaghosh invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT selvakumarramasamy invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT anbalaganmoorthy invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT skashokkumar invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT murugeshshivashankar invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines AT madhveshpathak invitroantiproliferativeevaluationofnewlysynthesizedtitaniumivmetallacycliccomplexesonhelaandmcf7celllines |