The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients

Abstract This study aims to evaluate the role of a specific gene polymorphism, Cytidine/Uridine Monophosphate Kinase 1 (CMPK1) rs1044457, in predicting the response to gemcitabine‐based chemotherapy in patients with NSCLC. A total of 98 NSCLC patients are enrolled in the study. Based on their respon...

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Main Authors: Ghassan Saod Saleh, Fouad Kadhim Gatea, Qasim Sharhan Al‐Mayah, Hayder Lazim
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Advanced Genetics
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Online Access:https://doi.org/10.1002/ggn2.202400058
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author Ghassan Saod Saleh
Fouad Kadhim Gatea
Qasim Sharhan Al‐Mayah
Hayder Lazim
author_facet Ghassan Saod Saleh
Fouad Kadhim Gatea
Qasim Sharhan Al‐Mayah
Hayder Lazim
author_sort Ghassan Saod Saleh
collection DOAJ
description Abstract This study aims to evaluate the role of a specific gene polymorphism, Cytidine/Uridine Monophosphate Kinase 1 (CMPK1) rs1044457, in predicting the response to gemcitabine‐based chemotherapy in patients with NSCLC. A total of 98 NSCLC patients are enrolled in the study. Based on their response, patients are classified as either responders or non‐responders. Specific primers are designed to amplify the rs1044457 variant and performed genotyping using restriction fragment length polymorphism (RFLP). The rs1044457 variant showed a statistically significant difference in frequency between responder and non‐responder patients. The mutant genotype (TT) is more frequent in non‐responder patients (18.75%) compared to responder patients (4%), with an odds ratio [OR] of 5.93 (95% confidence interval [CI] = 1.16–30.25, p = 0.032). Additionally, at the allelic level, the mutant allele (T) is more common in non‐responder patients (36.46%) compared to responder patients (23%), with a statistically significant odds ratio of 1.92 (95% CI = 1.03–3.58, p = 0.040). The findings of this study suggest that the mutant allele (allele T) of the rs1044457 variant may serve as a risk factor for resistance to gemcitabine‐based chemotherapy in patients with NSCLC.
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spelling doaj-art-2e739efad71b4404a126f99ade9c07c72025-08-20T03:49:50ZengWileyAdvanced Genetics2641-65732025-06-0162n/an/a10.1002/ggn2.202400058The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC PatientsGhassan Saod Saleh0Fouad Kadhim Gatea1Qasim Sharhan Al‐Mayah2Hayder Lazim3Ministry of Health, Salahuddin Health Directory Al‐Alam General Hospital Salahuddin IraqDepartment of Pharmacology and Therapeutics College of Medicine Al‐Nahrain University Baghdad IraqMedical Research Unit College of Medicine Al‐Nahrain University Baghdad IraqFaculty of Health Social Care and Medicine (FHSCM) School of Medicine Edge Hill University Ormskirk L39 4QP UKAbstract This study aims to evaluate the role of a specific gene polymorphism, Cytidine/Uridine Monophosphate Kinase 1 (CMPK1) rs1044457, in predicting the response to gemcitabine‐based chemotherapy in patients with NSCLC. A total of 98 NSCLC patients are enrolled in the study. Based on their response, patients are classified as either responders or non‐responders. Specific primers are designed to amplify the rs1044457 variant and performed genotyping using restriction fragment length polymorphism (RFLP). The rs1044457 variant showed a statistically significant difference in frequency between responder and non‐responder patients. The mutant genotype (TT) is more frequent in non‐responder patients (18.75%) compared to responder patients (4%), with an odds ratio [OR] of 5.93 (95% confidence interval [CI] = 1.16–30.25, p = 0.032). Additionally, at the allelic level, the mutant allele (T) is more common in non‐responder patients (36.46%) compared to responder patients (23%), with a statistically significant odds ratio of 1.92 (95% CI = 1.03–3.58, p = 0.040). The findings of this study suggest that the mutant allele (allele T) of the rs1044457 variant may serve as a risk factor for resistance to gemcitabine‐based chemotherapy in patients with NSCLC.https://doi.org/10.1002/ggn2.202400058CMPK1 gene polymorphismgemcitabine response ratenon‐small cell lung cancerrs1044457 polymorphism
spellingShingle Ghassan Saod Saleh
Fouad Kadhim Gatea
Qasim Sharhan Al‐Mayah
Hayder Lazim
The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
Advanced Genetics
CMPK1 gene polymorphism
gemcitabine response rate
non‐small cell lung cancer
rs1044457 polymorphism
title The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
title_full The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
title_fullStr The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
title_full_unstemmed The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
title_short The Impact of the Rs1044457 Polymorphism in the CMPK1 Gene on the Response Rate to Gemcitabine‐Based Chemotherapy in Metastatic NSCLC Patients
title_sort impact of the rs1044457 polymorphism in the cmpk1 gene on the response rate to gemcitabine based chemotherapy in metastatic nsclc patients
topic CMPK1 gene polymorphism
gemcitabine response rate
non‐small cell lung cancer
rs1044457 polymorphism
url https://doi.org/10.1002/ggn2.202400058
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