High resolution clonal architecture of hypomutated Wilms tumours

Abstract A paradigm of childhood cancers is that they have a low mutation burden, with some ostensibly bearing fewer mutations than the normal tissues from which they derive. We set out to resolve this paradox by examining paediatric renal cancers with exceptionally few mutations using high resoluti...

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Main Authors: Henry Lee-Six, Taryn D. Treger, Manas Dave, Tim HH Coorens, Nathaniel D. Anderson, Yvonne Tiersma, Sepide Derakhshan, Sanne de Haan, Marry M. van den Heuvel-Eibrink, Yichen Wang, Anna Wenger, Reem Al-Saadi, Alice Lawford, Aleksandra Letunovska, Jenny Wegert, Conor Parks, Guillaume Morcrette, Manfred Gessler, Gordan Vujanic, Tanzina Chowdhury, Maureen J O’Sullivan, Ronald R. de Krijger, Michael R. Stratton, Kathy Pritchard-Jones, J. Ciaran Hutchinson, Jarno Drost, Sam Behjati
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59854-4
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author Henry Lee-Six
Taryn D. Treger
Manas Dave
Tim HH Coorens
Nathaniel D. Anderson
Yvonne Tiersma
Sepide Derakhshan
Sanne de Haan
Marry M. van den Heuvel-Eibrink
Yichen Wang
Anna Wenger
Reem Al-Saadi
Alice Lawford
Aleksandra Letunovska
Jenny Wegert
Conor Parks
Guillaume Morcrette
Manfred Gessler
Gordan Vujanic
Tanzina Chowdhury
Maureen J O’Sullivan
Ronald R. de Krijger
Michael R. Stratton
Kathy Pritchard-Jones
J. Ciaran Hutchinson
Jarno Drost
Sam Behjati
author_facet Henry Lee-Six
Taryn D. Treger
Manas Dave
Tim HH Coorens
Nathaniel D. Anderson
Yvonne Tiersma
Sepide Derakhshan
Sanne de Haan
Marry M. van den Heuvel-Eibrink
Yichen Wang
Anna Wenger
Reem Al-Saadi
Alice Lawford
Aleksandra Letunovska
Jenny Wegert
Conor Parks
Guillaume Morcrette
Manfred Gessler
Gordan Vujanic
Tanzina Chowdhury
Maureen J O’Sullivan
Ronald R. de Krijger
Michael R. Stratton
Kathy Pritchard-Jones
J. Ciaran Hutchinson
Jarno Drost
Sam Behjati
author_sort Henry Lee-Six
collection DOAJ
description Abstract A paradigm of childhood cancers is that they have a low mutation burden, with some ostensibly bearing fewer mutations than the normal tissues from which they derive. We set out to resolve this paradox by examining paediatric renal cancers with exceptionally few mutations using high resolution, high depth sequencing approaches. We find that apparent hypomutation is the result of unusual clonal architecture due to a normal tissue-like mode of tumour evolution, raising the possibility that the mutation burden of some cancers has been systematically misjudged.
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spelling doaj-art-2e7335bbea9e4e88b916f29ff8c27ae12025-08-20T02:00:07ZengNature PortfolioNature Communications2041-17232025-05-0116111010.1038/s41467-025-59854-4High resolution clonal architecture of hypomutated Wilms tumoursHenry Lee-Six0Taryn D. Treger1Manas Dave2Tim HH Coorens3Nathaniel D. Anderson4Yvonne Tiersma5Sepide Derakhshan6Sanne de Haan7Marry M. van den Heuvel-Eibrink8Yichen Wang9Anna Wenger10Reem Al-Saadi11Alice Lawford12Aleksandra Letunovska13Jenny Wegert14Conor Parks15Guillaume Morcrette16Manfred Gessler17Gordan Vujanic18Tanzina Chowdhury19Maureen J O’Sullivan20Ronald R. de Krijger21Michael R. Stratton22Kathy Pritchard-Jones23J. Ciaran Hutchinson24Jarno Drost25Sam Behjati26Wellcome Sanger InstituteWellcome Sanger InstituteWellcome Sanger InstituteBroad Institute of MIT and HarvardWellcome Sanger InstitutePrincess Máxima Center for Pediatric OncologyPrincess Máxima Center for Pediatric OncologyPrincess Máxima Center for Pediatric OncologyPrincess Máxima Center for Pediatric OncologyWellcome Sanger InstituteWellcome Sanger InstituteUCL Great Ormond Street Institute of Child HealthGreat Ormond Street Hospital for ChildrenUCL Great Ormond Street Institute of Child HealthTheodor-Boveri-Institute/Biocenter, Developmental Biochemistry, Würzburg University & Comprehensive Cancer Center MainfrankenWellcome Sanger InstituteUCL Great Ormond Street Institute of Child HealthTheodor-Boveri-Institute/Biocenter, Developmental Biochemistry, Würzburg University & Comprehensive Cancer Center MainfrankenDepartment of Pathology, Sidra MedicineUCL Great Ormond Street Institute of Child HealthDepartment of Pathology, Children’s Health Ireland at CrumlinPrincess Máxima Center for Pediatric OncologyWellcome Sanger InstituteUCL Great Ormond Street Institute of Child HealthGreat Ormond Street Hospital for ChildrenPrincess Máxima Center for Pediatric OncologyWellcome Sanger InstituteAbstract A paradigm of childhood cancers is that they have a low mutation burden, with some ostensibly bearing fewer mutations than the normal tissues from which they derive. We set out to resolve this paradox by examining paediatric renal cancers with exceptionally few mutations using high resolution, high depth sequencing approaches. We find that apparent hypomutation is the result of unusual clonal architecture due to a normal tissue-like mode of tumour evolution, raising the possibility that the mutation burden of some cancers has been systematically misjudged.https://doi.org/10.1038/s41467-025-59854-4
spellingShingle Henry Lee-Six
Taryn D. Treger
Manas Dave
Tim HH Coorens
Nathaniel D. Anderson
Yvonne Tiersma
Sepide Derakhshan
Sanne de Haan
Marry M. van den Heuvel-Eibrink
Yichen Wang
Anna Wenger
Reem Al-Saadi
Alice Lawford
Aleksandra Letunovska
Jenny Wegert
Conor Parks
Guillaume Morcrette
Manfred Gessler
Gordan Vujanic
Tanzina Chowdhury
Maureen J O’Sullivan
Ronald R. de Krijger
Michael R. Stratton
Kathy Pritchard-Jones
J. Ciaran Hutchinson
Jarno Drost
Sam Behjati
High resolution clonal architecture of hypomutated Wilms tumours
Nature Communications
title High resolution clonal architecture of hypomutated Wilms tumours
title_full High resolution clonal architecture of hypomutated Wilms tumours
title_fullStr High resolution clonal architecture of hypomutated Wilms tumours
title_full_unstemmed High resolution clonal architecture of hypomutated Wilms tumours
title_short High resolution clonal architecture of hypomutated Wilms tumours
title_sort high resolution clonal architecture of hypomutated wilms tumours
url https://doi.org/10.1038/s41467-025-59854-4
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