Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes
Tropical grassland soils, especially those with alkaline properties, often exhibit limited phosphorus availability due to its precipitation in insoluble forms. Phosphate-solubilizing bacteria (PSB) and rhizobia have demonstrated their potential to enhance the availability of this nutrient and promot...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/11/2493 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850149592862031872 |
|---|---|
| author | Winston Franz Ríos-Ruiz Rony Castro-Tuanama Renzo Alfredo Valdez-Nuñez Lenin Torres-Bernal Henry Giovani Jave-Concepción Aurora Consuelo Daza-Pérez Marvin Barrera-Lozano Fernando Archentti-Reátegui |
| author_facet | Winston Franz Ríos-Ruiz Rony Castro-Tuanama Renzo Alfredo Valdez-Nuñez Lenin Torres-Bernal Henry Giovani Jave-Concepción Aurora Consuelo Daza-Pérez Marvin Barrera-Lozano Fernando Archentti-Reátegui |
| author_sort | Winston Franz Ríos-Ruiz |
| collection | DOAJ |
| description | Tropical grassland soils, especially those with alkaline properties, often exhibit limited phosphorus availability due to its precipitation in insoluble forms. Phosphate-solubilizing bacteria (PSB) and rhizobia have demonstrated their potential to enhance the availability of this nutrient and promote the growth of forage legumes. This study, conducted under controlled conditions in a mesh house, evaluated the effect of co-inoculation with PSB, including <i>Micrococcus</i> sp. Sfcm-14-01, <i>Agrobacterium</i> sp. Sfl-043-09, and <i>Enterobacter</i> sp. Sfcm-014-02 and Sfcm-054-06, along with rhizobia (<i>Ensifer terangae</i> R1-012-02 and <i>Bradyrhizobium glycinis</i> Rcm-025-01), under different levels of phosphorus fertilization on the legumes <i>Leucaena leucocephala</i> and <i>Centrosema macrocarpum</i>. The results indicate significant increases in various growth parameters, such as chlorophyll levels (SPAD), biomass (dry weight of roots and aerial parts) (mg), the foliar phosphorus concentration (ppm), and the concentration of available phosphorus in the soil, particularly under low-phosphorus fertilization conditions. The highest level of available phosphorus in the soil was achieved with 75% of the recommended fertilization dose, resulting in concentrations of 13.73 ppm for <i>L. leucocephala</i> and 7.69 ppm for <i>C. macrocarpum</i>, representing increases in phosphorus availability of 170.81% and 240.27%, respectively, compared with no fertilization or inoculation. These findings suggest that the co-inoculation of PSB and native rhizobia is a promising strategy to enhance the biomass productivity and mineral content of forage in tropical grazing systems, especially under phosphorus-limited conditions. |
| format | Article |
| id | doaj-art-2e656df2efbf40f9ab732e87efa28b3c |
| institution | OA Journals |
| issn | 2073-4395 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-2e656df2efbf40f9ab732e87efa28b3c2025-08-20T02:26:51ZengMDPI AGAgronomy2073-43952024-10-011411249310.3390/agronomy14112493Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage LegumesWinston Franz Ríos-Ruiz0Rony Castro-Tuanama1Renzo Alfredo Valdez-Nuñez2Lenin Torres-Bernal3Henry Giovani Jave-Concepción4Aurora Consuelo Daza-Pérez5Marvin Barrera-Lozano6Fernando Archentti-Reátegui7Laboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Investigación en Biotecnología, Departamento Académico de Ciencias Básicas y Afines, Facultad de Ingeniería, Universidad Nacional de Barranca, Barranca 15169, PeruDepartamento Académico Agrosilvopastoril, Escuela Profesional de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Botánica y Dendrología, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruLaboratorio de Microbiología Agrícola “Raúl Ríos Reátegui”, Departamento Académico Agrosilvopastoril, Facultad de Ciencias Agrarias, Universidad Nacional de San Martín, Tarapoto 22202, PeruTropical grassland soils, especially those with alkaline properties, often exhibit limited phosphorus availability due to its precipitation in insoluble forms. Phosphate-solubilizing bacteria (PSB) and rhizobia have demonstrated their potential to enhance the availability of this nutrient and promote the growth of forage legumes. This study, conducted under controlled conditions in a mesh house, evaluated the effect of co-inoculation with PSB, including <i>Micrococcus</i> sp. Sfcm-14-01, <i>Agrobacterium</i> sp. Sfl-043-09, and <i>Enterobacter</i> sp. Sfcm-014-02 and Sfcm-054-06, along with rhizobia (<i>Ensifer terangae</i> R1-012-02 and <i>Bradyrhizobium glycinis</i> Rcm-025-01), under different levels of phosphorus fertilization on the legumes <i>Leucaena leucocephala</i> and <i>Centrosema macrocarpum</i>. The results indicate significant increases in various growth parameters, such as chlorophyll levels (SPAD), biomass (dry weight of roots and aerial parts) (mg), the foliar phosphorus concentration (ppm), and the concentration of available phosphorus in the soil, particularly under low-phosphorus fertilization conditions. The highest level of available phosphorus in the soil was achieved with 75% of the recommended fertilization dose, resulting in concentrations of 13.73 ppm for <i>L. leucocephala</i> and 7.69 ppm for <i>C. macrocarpum</i>, representing increases in phosphorus availability of 170.81% and 240.27%, respectively, compared with no fertilization or inoculation. These findings suggest that the co-inoculation of PSB and native rhizobia is a promising strategy to enhance the biomass productivity and mineral content of forage in tropical grazing systems, especially under phosphorus-limited conditions.https://www.mdpi.com/2073-4395/14/11/2493foliar phosphoruschlorophyll levelaerial dry biomassphosphate solubilizationtropical grazing |
| spellingShingle | Winston Franz Ríos-Ruiz Rony Castro-Tuanama Renzo Alfredo Valdez-Nuñez Lenin Torres-Bernal Henry Giovani Jave-Concepción Aurora Consuelo Daza-Pérez Marvin Barrera-Lozano Fernando Archentti-Reátegui Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes Agronomy foliar phosphorus chlorophyll level aerial dry biomass phosphate solubilization tropical grazing |
| title | Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes |
| title_full | Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes |
| title_fullStr | Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes |
| title_full_unstemmed | Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes |
| title_short | Co-Inoculation of Phosphate-Solubilizing Bacteria and Rhizobia Increases Phosphorus Availability and Promotes the Development of Forage Legumes |
| title_sort | co inoculation of phosphate solubilizing bacteria and rhizobia increases phosphorus availability and promotes the development of forage legumes |
| topic | foliar phosphorus chlorophyll level aerial dry biomass phosphate solubilization tropical grazing |
| url | https://www.mdpi.com/2073-4395/14/11/2493 |
| work_keys_str_mv | AT winstonfranzriosruiz coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT ronycastrotuanama coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT renzoalfredovaldeznunez coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT lenintorresbernal coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT henrygiovanijaveconcepcion coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT auroraconsuelodazaperez coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT marvinbarreralozano coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes AT fernandoarchenttireategui coinoculationofphosphatesolubilizingbacteriaandrhizobiaincreasesphosphorusavailabilityandpromotesthedevelopmentofforagelegumes |